Tendon regeneration is greatly influenced by the oxidant and inflammatory microenvironment. Persistent inflammation during tendon repair can cause matrix degradation, adhesion, excessive accumulation of reactive oxygen species (ROS), while ROS affect extracellular remodeling integration. Herein, we used tannic acid (TA) to modify a decellularized slice (DTS) fabricate functional scaffold (DTS-TA) with antioxidant anti-inflammatory properties for repair. The characterizations...

Abstract To develop a naturally derived tendon tissue engineering scaffold with the preservation of native ultrastructure, tensile strength, and biochemical composition extracellular matrix (ECM), decellularized slices (DTSs) were prepared using repetitive freeze/thaw intact Achilles tendons, frozen section, nuclease treatment. The DTSs characterized in mechanical properties, composition, cytocompatibility. Histological examination DNA quantification analysis confirmed that cells completely...

Tendon regeneration highly relies on biomechanical and biochemical cues in the repair microenvironment. Herein, we combined decellularized bovine tendon sheet (DBTS) with extracellular matrix (ECM) from tendon-derived stem cells (TDSCs) to fabricate a biomechanically biochemically functional scaffold (tECM-DBTS), provide cell ECM-based microenvironment for regeneration. Our prior study showed that DBTS was suitable repair. In this study, biological function of tECM-DBTS examined vitro,...

Mimicking the native microenvironment is vital for tumor engineering. Breast cancer a highly heterogeneous disease with various molecular subtypes exhibiting distinct biological behaviors and treatment responsiveness. The heterogeneity of extracellular matrix (ECM) breast has remained largely unexplored underestimated. present study addressed this issue by comparing composition, architecture, functional roles ECMs derived from cancers two subtypes, which are luminal-A (less aggressive,...

The selection of appropriate cell sources is vital for the regeneration and repair tendons using stem cell-based approaches. Human adipose-derived cells (hADSCs) have emerged as a promising therapeutic strategy tendon injuries. However, heterogeneity hADSCs can lead to inconsistent or suboptimal outcomes. In this study, we isolated identified tenomodulin (TNMD)-positive subpopulation from (TNMD

Background: Poor healing of the tendon-bone interface after rotator cuff repair is one main causes surgical failure. Previous studies demonstrated that demineralized cortical bone (DCB) could improve enthesis. Purpose: To evaluate outcomes hierarchically (hDCB) coated with stem cell–derived extracellular matrix (hDCB-ECM) in a rabbit model. Study Design: Controlled laboratory study. Methods: Tendon-derived cells (TDSCs) were isolated, cultured, and identified. Then, hDCB was prepared by...

Obtaining a performing decellularized tendon scaffold with proper dimensions and adequate availability is highly desirable. However, the combined study of complete decellularization detailed characterization native extracellular matrix (ECM) from large animals still lacking. In present study, we developed new protocol, including physical methods enzymatic solutions for processing bovine Achilles tendons, produced sheet (DBTS) reconstruction. The effectiveness was demonstrated by DNA...

Decellularized tendon hydrogel from human or porcine has been manufactured and found to be capable of augmenting repair in vivo . However, no studies have clarified the effect decellularized upon stem cell behavior. In present study, we developed a new (T-gel) Macaca mulatta, investigated T-gel on proliferation, migration tenogenic differentiation mulatta tendon-derived cells (mTDSCs). The mTDSCs were first identified universal characteristics, including clonogenicity, expression mesenchymal...

Bioadhesives have been widely used in hemostasis and tissue repair, but the overmoist wet nature of wound surface (due to presence blood and/or exudate) has led poor adhesion bioadhesives, which interrupts continuous care wounds. Here, a thirsty polyphenolic silk granule (Tan@SF-pwd-hydro), absorbs exudate self-convert robust bioadhesives (Tan@SF-gel-hydro) situ, was facilely developed this study for enhanced toward repair. Tan@SF-pwd-hydro could shield wounds' wetness immediately convert...

Background: Achilles tendon (AT) defects frequently occur in trauma and chronic injuries. Currently, no method can satisfactorily reconstruct the AT with completely restored function. Purpose: To evaluate postoperative outcomes of defect reconstruction decellularized bovine sheets (DBTSs) a rabbit model. Study Design: Controlled laboratory study. Methods: DBTSs were prepared from tendons after compression, decellularization, antigen extraction, freeze drying, sterilization. Platelet-rich...

Biglycan (BGN) has been identified as one of the critical components tendon-derived stem cells (TDSCs) niche and may be related to tendon formation. However, so far, no study demonstrated whether soluble BGN could induce tenogenic differentiation TDSCs in vitro. The aim this was investigate effect on TDSCs. proliferation exposed different concentrations (0, 50, 100, 500 ng/ml) were determined by live/dead cell staining assay, CCK-8 quantitative real-time polymerase chain reaction (qRT-PCR),...

Demineralized bone matrix (DBM), as an extracellular (ECM), has had limited use a medical replacement although studies have reported possibility for its in tendon or ligament tissue engineering. To be acid-extracted organic matrix, DBM contains much of protein, with small amount inorganic solids and some cell debris. However, debris is critical factor that triggers inflammatory reaction clinical reconstructions using ECM. In this study, we used protocol incorporating the detergent nuclease...

It is highly desirable to develop a novel scaffold that can induce stem cell migration in tendon tissue engineering and regeneration. The objective of this study assess the effect extracellular matrix-modified decellularized slices (ECM-DTSs) on bone marrow mesenchymal cells (BMSCs) explore possible molecular mechanisms. Native ECM produced by BMSCs tendon-derived (TDSCs) was deposited DTSs, denoted as bECM-DTSs tECM-DTSs, respectively, treated with extracts from ECM-DTSs studied. Almost all...

Due to the similar collagen composition and closely physiological relationship with soft connective tissues, demineralized bone matrices (DBMs) were used repair injured tendon or ligament. However, osteoinductivity of DBMs would be a huge barrier these applications. Hydrogen peroxide (H2 O2 ) has been proved reduce DBMs. Nevertheless, biological properties H2 -treated have not evaluated completely, while potential mechanism compromising is also unclear. Hence, purpose this study was...

Abstract Recent studies have indicated that demineralized cortical bone (DCB) may be used to repair tendons and ligaments, such as the patellar tendon anterior cruciate ligament (ACL). Hydrogen peroxide (H2O2) has been shown reduce osteoinductivity of DCB, heat treatment also decrease DCB. The purpose this study was (i) determine whether reduces DCB (ii) compare effectiveness H2O2 on BMP-2 inactivation. prepared by immersion in 0.6 N hydrochloric acid, DCB-H DCB-HO were (70°C for 8 h) (3%...

Abstract The biomechanical characteristics of tendon grafts is essential for reconstructive surgery due to its great role in providing a good mechanical environment healing and regeneration. In our previous studies, the decellularized slices (DTSs) bovine sheets (DBTSs) scaffolds were successfully developed. However, influence integrity tendinous membrane (endotenon epitenon) fascicle on these two was not investigated. this study, we assessed derived effect characteristics. results...

A recent study has shown that demineralized cortical bone (DCB) did not improve the healing of tendon-bone interface. Considering there is a gradient mineral content in interface, we designed segmentally (sDCB) scaffold with two different regions: undemineralized section within (sDCB-B) and complete (sDCB-D), to mimic natural structure Furthermore, extracellular matrix (ECM) from tendon-derived stem cells (TDSCs) was used modify sDCB-D region sDCB construct novel (sDCB-ECM) for enhancing...