A5017638929- Cancer Genomics and Diagnostics
- Neonatal Respiratory Health Research
- Cancer Cells and Metastasis
- Ovarian cancer diagnosis and treatment
- Genomic variations and chromosomal abnormalities
- Retinopathy of Prematurity Studies
- Gene expression and cancer classification
- Cancer-related molecular mechanisms research
- Prenatal Screening and Diagnostics
- Neonatal and fetal brain pathology
- Mitochondrial Function and Pathology
- Epigenetics and DNA Methylation
- Molecular Biology Techniques and Applications
- Genetic factors in colorectal cancer
- Infant Nutrition and Health
- Cancer-related Molecular Pathways
- Plant tissue culture and regeneration
- Neuroscience of respiration and sleep
- Single-cell and spatial transcriptomics
- Plant Molecular Biology Research
- Congenital Diaphragmatic Hernia Studies
- interferon and immune responses
- Gut microbiota and health
- Chromosomal and Genetic Variations
- Colorectal Cancer Screening and Detection
Oslo University Hospital
2014-2023
Østfold University College
2023
University of Oslo
2001-2019
Norwegian Cancer Society
2005-2009
Graz University of Technology
2009
University of Freiburg
1998-1999
Institute of Bioinformatics and Systems Biology
1998
Fraunhofer Society
1998
Abstract Background Cancer progression is associated with genomic instability and an accumulation of gains losses DNA. The growing variety tools for measuring copy numbers, including various types array-CGH, SNP arrays high-throughput sequencing, calls a coherent framework offering unified consistent handling single- multi-track segmentation problems. In addition, there demand highly computationally efficient algorithms, due to the emergence very high density scans number. Results A...
SET domains are conserved amino acid motifs present in chromosomal proteins that function epigenetic control of gene expression. These can be divided into four classes as typified by their Drosophila members E(Z), TRX, ASH1 and SU(VAR)3-9. Homologs all have been identified yeast mammals, but not plants. A BLASTP screening the Arabidopsis genome 37 genes: three E(z) homologs, five trx ash1 homologs 15 genes similar to Su(var)3-9. Seven were assigned trx‐related ash1-related. Only described...
Abstract Summary: CGH-Explorer is a program for visualization and statistical analysis of microarray-based comparative genomic hybridization (array-CGH) data. The has preprocessing facilities, tools graphical exploration individual arrays or groups arrays, identification regions amplification deletion. Availability: available as Java class files that runs on any platform with the 2 runtime environment (J2SE JRE) installed, Windows executable. source are also available. See...
This study demonstrates the relation among structural genomic alterations, molecular subtype, and clinical behavior shows that an objective score of complexity can provide independent prognostic information in breast cancer.
Clinical DNA is often available in limited quantities requiring whole-genome amplification for subsequent genome-wide assessment of copy-number variation (CNV) by array-CGH. In pre-implantation diagnosis and analysis micrometastases, even merely single cells are analysis. However, procedures allowing high-resolution analyses CNVs from well below resolution limits conventional cytogenetics lacking. Here, we applied products cell pools (5 or 10 cells) patients with developmental delay, cancer...
Abstract Introduction The detection of circulating tumour cells (CTCs) in the peripheral blood and disseminated (DTCs) bone marrow are promising prognostic tools for risk stratification early breast cancer. There is, however, a need further validation these techniques larger patient cohorts with adequate follow-up periods. Methods We assayed CTCs DTCs at primary surgery 733 stage I or II cancer patients median time 7.6 years. were detected samples mononuclear previously stored...
Single-cell micro-metastases of solid tumors often occur in the bone marrow. These disseminated tumor cells (DTCs) may resist therapy and lay dormant or progress to cause overt visceral metastases. The molecular nature DTCs remains elusive, as well when from where they originate. Here, we apply single-cell sequencing identify trace origin breast cancer. We sequence genomes 63 single isolated six non-metastatic cancer patients. By comparing cells' DNA copy number aberration (CNA) landscapes...
Genomic copy number alterations are common in cancer. Finding the genes causally implicated oncogenesis is challenging because gain or loss of a chromosomal region may affect few key driver and many passengers. Integrative analyses have opened new vistas for addressing this issue. One approach to identify with frequent corresponding changes expression. Several methods also analyse effects transcriptional on known pathways. Here, we propose method that in-cis correlated evidence in-trans...
Microarray Comparative Genomic Hybridization (array CGH) provides a means to examine DNA copy number aberrations. Various platforms, brands and underlying technologies are available, facing the user with many choices regarding platform sensitivity number, localization, density distribution of probes. We evaluate three different platforms presenting nature arrangement probes: The Agilent Human Genome CGH 44 k, ROMA/NimbleGen Representational Oligonucleotide 82 Illumina Human-1 Genotyping 109...
The presence of disseminated tumor cells (DTCs) in bone marrow (BM) identifies breast cancer patients with less favorable outcome. Furthermore, molecular characterization is required to investigate the malignant potential these cells. This study presents a single-cell array comparative genomic hybridization (SCaCGH) method providing analysis immunomorphologically detected DTCs. resolution limit was estimated using cell line SK-BR-3 on 44 and 244k arrays. technique further tested 28...
Breast cancer, the second leading cause of cancer death in women, is a highly heterogeneous disease, characterized by distinct genomic and transcriptomic profiles. Transcriptome analyses prevalently assessed protein-coding genes; however, majority mammalian genome expressed numerous non-coding transcripts. Emerging evidence supports that many these RNAs are specifically during development, tumorigenesis, metastasis. The focus this study was to investigate expression features molecular...
Disseminated tumor cells (DTCs) detected in the bone marrow have been shown as an independent prognostic factor for women with breast cancer. However, mechanisms behind cell dissemination are still unclear and more detailed knowledge is needed to fully understand why some remain dormant others metastasize. Sequencing of single has opened possibility dissect genetic content subclones a primary tumor, well DTCs. Previous studies changes DTCs employed single-cell array comparative genomic...
Dormant Arabidopsis seeds require stratification and light for germination. To study gene expression during establishment, maintenance release of dormancy, various ecotypes that are different in their degree dormancy were investigated; three nsm mutants lack the stratification-dependency, precocious germination reduced abi3-1 mutant (insensitive to ABA). Genes examined by mRNA abundance include LEC1, FUS3 ABI3, transcription factors major regulators embryo development and, at least...
Genomic instability and copy number alterations in cancer are generally associated with poor prognosis; however, recent studies have suggested that extreme levels of genomic aberrations may be beneficial for the survival outcome patients specific tumour types. We investigated extent predominantly high-grade serous ovarian cancers (SOC) using two independent datasets, generated Norway (n = 74) Australia 70), respectively. was quantified by Total Aberration Index (TAI), a measure abundance...
There is a lack of reliable biomarkers that can identify and grade acute hypoxic-ischemic encephalopathy in newborns. MicroRNAs (miRNA) are short, non-coding strands RNA released into the circulation response to tissue stress injury. Some miRNAs highly specific thus may potentially be non-invasive neonatal brain injury.The aim this study was characterize temporal expression selected circulating clinically relevant piglet model hypoxia-ischemia (HI).A total 13 anesthetized newborn piglets...
Objective Total tau (T-tau), phosphorylated (p-Tau) and Beta-Amyloid 1–42 (AB42) in Cerebrospinal Fluid (CSF) are useful biomarkers neurodegenerative diseases. The aim of the study was to investigate role these other CSF (T-tau, p-Tau, AB42, S100B NSE), during hypoxia-reoxygenation a newborn pig model. Design Thirty pigs were included moderate or severe hypoxia. hypoxia group (n = 12) exposed global (8% O2) until Base excess (BE) reached -15 mmol/l. received 8% O2 BE -20 mmol/l mean Blood...
Detection and localization of genomic alterations breakpoints are crucial in cancer research. The purpose this study was to investigate, a methodological biological perspective, different female, hormone-dependent cancers identify common diverse DNA aberrations, genes, pathways. In work, we analyzed tissue samples from patients with breast (n = 112), ovarian 74), endometrial 84), or cervical 76) cancer. To the Circular Binary Segmentation (CBS) Piecewise Constant Fitting (PCF) algorithms...
<b><i>Background:</i></b> Bronchopulmonary dysplasia (BPD) is a common cause of abrupted lung development after preterm birth. BPD may lead to increased rehospitalization, more severe and frequent respiratory infections, life-long reduced function. The gene regulation in lungs with complex, various genetic epigenetic factors involved. <b><i>Objectives:</i></b> aim this study was examine the regulatory relation between expression epigenome (DNA...
Abstract Mammary carcinomas developing in SV40 transgenic WAP‐T mice arise two distinct histological phenotypes: as differentiated low‐grade and undifferentiated high‐grade tumors. We integrated different types of information such grading, analysis aCGH‐based gene copy number expression profiling to provide a comprehensive molecular description mammary tumors mice. Applying novel procedure for the correlation with on global scale, we observed tumor samples coherence between genotype...
In this study, we aimed to analyze differences in plasma protein abundances between infants with and without bronchopulmonary dysplasia (BPD), add new insights into a better understanding of the pathogenesis disease. Cord peripheral blood neonates (≤ 30 weeks gestational age) was drawn at birth 36th postmenstrual week (36 PMA), respectively. Blood samples were retrospectively subdivided BPD(+) BPD(−) groups, according development BPD. Children BPD characterized by decreased afamin, gelsolin...
To study the role of low UV-B radiation in modulating response antioxidants to ozone, 4-year-old pine (Pinus sylvestris L.) and spruce (Picea abies seedlings potted natural soil, were exposed phytochambers fluctuating ozone concentrations between 9 113 nl 1-1 according field data recorded at Mt Wank (1175 m above sea level, Bavaria, Germany) two-times ambient O3 levels. was either added a biologically effective level ca 1.2 kJ m-2 day-1 , which is close that found March Wank, or excluded by...
The tumor suppressor gene p53 (TP53) controls numerous signaling pathways and is frequently mutated in human cancers. Novel isoforms suggest alternative splicing as a regulatory feature of activity.In this study we have analyzed mRNA expression both wild-type its respective Deltap53 isoform 88 samples from breast cancer relation to clinical parameters molecular subgroups. Three-dimensional structure differences for the novel internally deleted been predicted. We confirmed tumors using...