A5021878254- Neonatal Respiratory Health Research
- Nitric Oxide and Endothelin Effects
- Aldose Reductase and Taurine
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Endometrial and Cervical Cancer Treatments
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Uterine Myomas and Treatments
- Immune Response and Inflammation
- Virus-based gene therapy research
- Cervical Cancer and HPV Research
- Congenital Diaphragmatic Hernia Studies
- RNA modifications and cancer
- Immunodeficiency and Autoimmune Disorders
- Retinopathy of Prematurity Studies
- Infant Nutrition and Health
- Hematopoietic Stem Cell Transplantation
- vaccines and immunoinformatics approaches
- Mitochondrial Function and Pathology
- Epigenetics and DNA Methylation
- Endometriosis Research and Treatment
- T-cell and B-cell Immunology
- Prenatal Screening and Diagnostics
- Preterm Birth and Chorioamnionitis
- Multiple and Secondary Primary Cancers
Jagiellonian University
2000-2023
Heidelberg University
2012-2023
Gdańsk Medical University
2019
German Cancer Research Center
2012-2017
Uniwersytecki Szpital Dziecięcy
2016
University of Gdańsk
2010
Abstract Taurine (Tau) is an exceptionally abundant free amino acid in the cytosol of inflammatory cells and especially neutrophils. protects from self-destruction during processes that generate oxidants. The major function Tau leukocytes to trap chlorinated oxidants (HOCl). reacts with HOCl produce long-lived compound taurine chloramine (TauCl). Previously, we have shown other products neutrophil chlorinating system are able modify functions macrophages. In this study, investigated vitro...
Human papillomavirus (HPV) needs to persist in squamous epithelia for a certain amount of time complete its reproductive cycle. Therefore, the virus has evolved multiple immune evasion strategies. The interplay these mechanisms with host system decides whether HPV infection is cleared or becomes persistent. Clearance HPV-induced lesions mediated by cellular response, consisting both cytotoxic T lymphocyte and helper cell responses. Persistent infection, on other hand, single most important...
Summary Taurine chloramine (TauCl) is a major generated in activated neutrophils as result of the reaction highly toxic hypochlorous acid and taurine, most abundant free amino cytosol. In this study we have tested influence TauCl on properties murine dendritic cells (DC), cell population involved initiation an adaptive immune response against pathogenic organisms. N418 + , MHC II B7‐2 cells, from mouse bone marrow cultured presence granulocyte–macrophage colony‐stimulating factor, were...
Immune evasion of tumors poses a major challenge for immunotherapy. For human papillomavirus (HPV)-induced malignancies, multiple immune mechanisms have been described, including altered expression antigen processing machinery (APM) components. These changes can directly influence epitope presentation and thus T-cell responses against tumor cells. To date, the APM had not studied systematically in large array HPV+ samples. Therefore this study, systematic analysis was performed on mRNA...
Objective To examine whether taurine (Tau) or its physiologic chlorinated derivative, chloramine (Tau-Cl), affects proliferation of, and proinflammatory cytokine (interleukin-6 [IL-6] IL-8) production by, fibroblast-like synoviocytes (FLS) isolated from rheumatoid arthritis (RA) patients. Methods FLS, the synovial tissue of 19 RA patients cultured in vitro for 3–6 passages, were stimulated with recombinant human cytokines IL-1β (1 ng/ml), tumor necrosis factor α (TNFα; 10 IL-17 (10 ng/ml)...
<b><i>Background:</i></b> Bronchopulmonary dysplasia (BPD) is a common cause of abrupted lung development after preterm birth. BPD may lead to increased rehospitalization, more severe and frequent respiratory infections, life-long reduced function. The gene regulation in lungs with complex, various genetic epigenetic factors involved. <b><i>Objectives:</i></b> aim this study was examine the regulatory relation between expression epigenome (DNA...
Taurine chloramine (TauCl) is produced during inflammation by reaction of hypochlorous acid (HOCl) with taurine, the most abundant free amino in neutrophils. We previously reported that TauCl inhibits generation macrophage inflammatory mediators such as nitric oxide, prostaglandin E 2 (PGE ),tumour necrosis factor‐α (TNF‐α) and interleukin‐6 (IL‐6). In this study, activity modulating T‐cell activation was investigated. Treatment T cells (0·1–0·3 mm), prior to activation, found inhibit...
Abstract Background We aimed to identify global blood and retinal gene expression patterns in murine oxygen-induced retinopathy (OIR), a common model of prematurity, which may allow better understanding the pathogenesis this severe ocular prematurity complication identification potential biomarkers. Methods A total 120 C57BL/6J mice were randomly divided into an OIR group, 7-day-old pups maintained 75% oxygen for 5 days, or control group. RNA was extracted from whole-blood mononuclear cells...
Abstract Nitric oxide (NO) can have both effector (cytotoxic) and regulatory roles in immune function. In this study, we re-examined the potential role of nitric mediating macrophage-dependent suppression IL-2 synthesis. our model, TNP-specific CD4+ T cells are cocultured with Ag either peritoneal or alveolar macrophages. Both populations macrophages after vitro stimulation IFN-gamma inhibit release. also induces substantial levels NO release by these macrophages, as well high prostaglandin...
Adipokines and gastrointestinal tract hormones are important metabolic parameters, both epigenetic factors differential gene expression patterns may be associated with the alterations in their concentrations children. The function of FTO (FTO alpha-ketoglutarate dependent dioxygenase) regulation global rate is well described, whereas influence protooncogene PLAG1 (PLAG1 zinc finger) still not fully understood. A cross-sectional study on a group 26 children various BMI values (15.3-41.7;...
Local tissue macrophages are known to play a key role in regulation of adaptive immune responses, often by inhibition T‐cell activation and proliferation. In this study, we compare the influence alveolar peritoneal on T‐cell‐dependent interleukin‐2 (IL‐2) release. Alveolar macrophages, contrast enhance IL‐2 Assay panel potential macrophage‐derived mediators indicated that activated stimulated greater release IL‐1 β , tumour necrosis factor‐ α and, especially, leukotriene B 4 (>100 times)...
Field isolates of bovine leukaemia virus (BLV) show the presence a few amino acid substitutions in major conformational G and H epitopes on surface glycoprotein gp51. Potentially, these can affect 3D structure leading to their diminished immunoreactivity. The aim this study was express three gp51 glycoproteins carrying mutated as recombinant baculovirus proteins insect cells test immunoreactivity with sera.Env gene chimeras encoding env backbone BLV FLK strain were constructed, cloned into...
Mutations in RAG1 gene may result different types of severe combined immunodeficiencies. In this study, we compare clinical symptoms and laboratory findings four children with identical mutation gene. All analyzed patients presented immunodeficiencies associated or not Omenn syndrome (OS) features. our two variants were detected. The first the was deletion AA dinucleotide at position c.256_257 (p.Lys86ValfsTer33), second variant substitution c.2867T>C (p.Ile956Thr). Patient 1 detected that...
Abstract Transient hypogammaglobulinemia of infancy (THI) is one the most common forms in early childhood. THI usually associated with chronic, recurrent bacterial and viral infections, life-threatening some cases, yet its pathogenesis still largely unknown. As our previous findings indicated possible role Treg cells pathomechanism THI, aim current study was to investigate gene expression profile isolated from patients. The transcriptome-wide profiling performed using microarray technology...