A5061452520- COVID-19 Clinical Research Studies
- Sepsis Diagnosis and Treatment
- Neurological diseases and metabolism
- Neonatal and Maternal Infections
- Long-Term Effects of COVID-19
- Single-cell and spatial transcriptomics
- T-cell and B-cell Immunology
- SARS-CoV-2 and COVID-19 Research
- Immune Response and Inflammation
- Blood properties and coagulation
- Respiratory viral infections research
- Microfluidic and Bio-sensing Technologies
- Immune cells in cancer
- Chronic Lymphocytic Leukemia Research
- Neuropeptides and Animal Physiology
- Pneumonia and Respiratory Infections
- SARS-CoV-2 detection and testing
- Platelet Disorders and Treatments
- Cell Adhesion Molecules Research
- Cytokine Signaling Pathways and Interactions
- Immune Cell Function and Interaction
- COVID-19 Impact on Reproduction
- Blood groups and transfusion
- Intensive Care Unit Cognitive Disorders
- Immunotherapy and Immune Responses
Atotech (United States)
2024
Viotech Communications (France)
2019-2021
Beckman Coulter Foundation
2016-2019
Aix-Marseille Université
2018
Institut National de Recherche en Santé Publique
2004
Centre d’Immunologie de Marseille-Luminy
1998-2004
Inserm
1998-2004
The Netherlands Cancer Institute
2004
Centre National de la Recherche Scientifique
1998-2001
Institut Louis Malardé
1995
Pantetheinase (EC 3.5.1.‐) is an ubiquitous enzyme which in vitro has been shown to recycle pantothenic acid (vitamin B5) and produce cysteamine, a potent anti‐oxidant. We show that the Vanin‐1 gene encodes pantetheinase widely expressed mouse tissues: (1) activity specifically by transfectants immunodepleted specific antibodies; (2) GPI‐anchored pantetheinase, consequently ectoenzyme; (3) null mice are deficient membrane‐bound kidney liver; (4) these organs, major metabolic consequence...
An unbiased approach to SARS-CoV-2-induced immune dysregulation has not been undertaken so far. We aimed identify previously unreported markers able discriminate COVID-19 patients from healthy controls and predict mild severe disease.An observational, prospective, multicentric study was conducted in with confirmed mild/moderate (n = 7) 19) COVID-19. Immunophenotyping of whole-blood leukocytes performed upon hospital ward or intensive care unit admission 25). Clinically relevant associations...
Vanin-1 is an epithelial ectoenzyme with pantetheinase activity and generating the amino-thiol cysteamine through metabolism of pantothenic acid (vitamin B(5)). Here we show that Vanin-1(-/-) mice, which lack in tissues, exhibit resistance to oxidative injury induced by whole-body gamma-irradiation or paraquat. This protection correlated reduced apoptosis inflammation reversed treating mutant animals cystamine. The better tolerance mice associated enhanced gamma-glutamylcysteine synthetase...
We assessed the expression of CD169, a type I interferon-inducible receptor, on monocytes (monocyte CD169 [mCD169]) in 53 adult patients admitted to hospital during coronavirus disease 2019 (COVID-19) outbreak for suspicion severe acute respiratory syndrome 2 infection. Monocyte was strongly overexpressed 30 32 (93.7%) confirmed COVID-19 cases, compared with 3 21 (14.3%) whom diagnosis finally ruled out. associated plasma interferon-alpha level and thrombocytopenia. testing may be helpful...
The phosphatase and tensin homologue deleted on chromosome 10 (PTEN) negatively regulates cell survival proliferation mediated by phosphoinositol 3 kinases. We have explored the role of phosphoinositol(3,4,5)P3-phosphatase PTEN in T development analyzing mice with a cell–specific deletion PTEN. Ptenflox/floxLck-Cre developed thymic lymphomas, but before onset tumors, they showed normal cellularity. To reveal regulatory developing cells we crossed PTEN-deficient deficient for interleukin...
Vanin-1 is a membrane-anchored pantetheinase highly expressed in the gut and liver. It hydrolyzes pantetheine to pantothenic acid (vitamin B5) low-molecular-weight thiol cysteamine. The latter believed be key regulating factor of several essential metabolic pathways, acting through sulfhydryl-disulfide exchange reactions between sulfhydryl groups enzymes oxidized form, cystamine. Its physiological importance remains elucidated, however. To explore this point, we developed Vanin-1–deficient...
Abstract Introduction CD64 expression increases on neutrophils during bacterial infections. Recently an increase in CD169 has been discovered monocytes viral Generally, interferons α (IFNsα) and IFNsγ are key drivers of the infectious host immune response. The purpose this study was to explore if a link exists between these IFNs both biomarkers. Methods Whole blood samples from healthy volunteers were stimulated with either IFNs, interleukins, or extracts, mimic state. Expressions assessed...
Abstract The identification of a bacterial, viral, or even noninfectious cause is essential in the management febrile syndrome emergency department (ED), especially epidemic contexts such as flu CoVID‐19. aim was to assess discriminative performances two biomarkers, CD64 on neutrophils (nCD64) and CD169 monocytes (mCD169), using new flow cytometry procedure, patients presenting with fever ED during epidemics. Eighty five adult potential infection were included 2019 season La Timone Hospital....
Circulating Tumor Cells (CTCs) represent an easy, repeatable and representative access to information regarding solid tumors. However, their detection remains difficult because of paucity, short half-life, the lack reliable surface biomarkers. Flow cytometry (FC) is a fast, sensitive affordable technique, ideal for rare cells detection. Adapted CTCs (i.e. extremely cells), most FC-based techniques require time-consuming pre-enrichment step, followed by 2-hours staining procedure, impeding on...
Congenital macrothrombocytopenia is a family of rare diseases, which significant fraction remains to be genetically characterized. To analyze cases unexplained thrombocytopenia, 27 individuals from patient cohort the Bleeding and Thrombosis Exploration Center University Hospital Marseille were recruited for high-throughput gene sequencing study. This strategy led identification two novel FLI1 variants (c.1010G>A c.1033A>G) responsible macrothrombocytopenia. The variant carriers' platelets...
Abstract In adults, monocytes and neutrophils play important roles in the hyperinflammatory responses characteristic of severe forms SARS-CoV-2 infection. We assessed leukocyte activation 55 children attending emergency department for acute fever between March 2020 September 2021. The following markers were analyzed by flow cytometry: CD169 HLA-DR on monocytes, CD64 CD16 neutrophils, CD38 lymphocytes TCD8. Fifteen had infection, 15 bacterial infections, inflammatory diseases. observed...
Aim: Management of patients with infections within the Emergency Department (ED) is challenging for practitioners, as identification infectious causes remains difficult current techniques. A new combination two biomarkers was tested a rapid flow cytometry technique. Materials & methods: Subjects from ED were their CD64 on neutrophils (nCD64) and CD169 monocytes (mCD169) levels results compared to clinical records. Results: Among 139 patients, 29% had confirmed bacterial 5% viral infections....
The monitoring of septic shock induced immunosuppression has been proposed to identify patients who could benefit from specific immunoadjuvant therapies. Among potential biomarkers monitor immunological status, functional testing remains the gold standard because it directly measures capacity a cell population respond an immune challenge. We investigated new approach in intracellular staining for flow cytometry measure tumor necrosis factor (iTNF) produced vitro by monocytes response...
Aim: In an Emergency Department (ED), the etiological identification of infected subjects is essential. 13 infection-related biomarkers were assessed using a new flow cytometry procedure. Materials & methods: If presented with febrile symptoms at ED, biomarkers' levels, including CD64 on neutrophils (nCD64) and CD169 monocytes (mCD169), tested compared clinical records. Results: Among 50 subjects, 78% had bacterial infections 8% viral infections. nCD64 showed 82% sensitivity 91% specificity...
Vanin-1 is a membrane-anchored pantetheinase highly expressed in the gut and liver. It hydrolyzes pantetheine to pantothenic acid (vitamin B5) low-molecular-weight thiol cysteamine. The latter believed be key regulating factor of several essential metabolic pathways, acting through sulfhydryl-disulfide exchange reactions between sulfhydryl groups enzymes oxidized form, cystamine. Its physiological importance remains elucidated, however. To explore this point, we developed Vanin-1-deficient...
Abstract We assessed the expression of cell adhesion molecule Sialoadhesin (CD169), a type I interferon-inducible receptor, on monocytes (mCD169) in 53 adult patients admitted to hospital during COVID-19 outbreak for suspicion SARS-CoV-2 infection. mCD169 was strongly overexpressed 30 out 32 (93.7%) confirmed cases, compared three 21 (14.3%) whom diagnosis finally ruled out. associated with plasma interferon alpha level and thrombocytopenia. testing may be helpful rapid triage suspected an outbreak.
Abstract Blood cell analysis is a major pillar of biomedical research and healthcare. These analyses are performed in central laboratories. Rapid shipment from collection site to the laboratories currently needed because cells biomarkers degrade rapidly. The dried blood spot fingerstick allows preservation cellular molecules for months but entire never recovered. Here leucocyte elution optimized spots. Flow cytometry mRNA expression profiling used analyze recovered cells. 50–70% leucocytes...
Growth hormone (GH) and insulin‐like growth factor‐1 (IGF1) play an important role in mammalian development, cell proliferation lifespan. Especially cases of tumor there is urgent need to control the GH/IGF1 axis. In this study we screened a 38,480-compound library, two consecutive rounds analogues selection, identified active lead compounds based on following criteria: inhibition GH receptor (GHR) activity its downstream effectors Jak2 STAT5, breast colon cancer cells. The most small...
A strong bias related to age is observed in COVID-19 patients with pediatric subjects developing a milder disease than adults. We hypothesized that specific SARS-CoV-2 effect conjugated preexisting differences the immune systems may explain this. Using flow cytometry, we investigated basal cohort consisting of 16 non-infected young and aged individuals further leveraged an vitro whole blood model infection so functional could be mined as well. In short, diluted culture media was incubated 5...