A5070832607- Mesenchymal stem cell research
- Bone Tissue Engineering Materials
- Nerve injury and regeneration
- Tendon Structure and Treatment
- Periodontal Regeneration and Treatments
- Dental Implant Techniques and Outcomes
- Tissue Engineering and Regenerative Medicine
- Wound Healing and Treatments
- Electrospun Nanofibers in Biomedical Applications
- biodegradable polymer synthesis and properties
- Additive Manufacturing and 3D Printing Technologies
- Orthopaedic implants and arthroplasty
- Veterinary Equine Medical Research
- Tracheal and airway disorders
- Silk-based biomaterials and applications
- Pluripotent Stem Cells Research
- Veterinary Orthopedics and Neurology
- Dental materials and restorations
- Nerve Injury and Rehabilitation
- Agricultural Engineering and Mechanization
- Extracellular vesicles in disease
- Cancer, Hypoxia, and Metabolism
- Neurogenetic and Muscular Disorders Research
- Soil Mechanics and Vehicle Dynamics
- Scientific and Engineering Research Topics
Universidade do Porto
2018-2024
University of Lisbon
2022
Peripheral nerve lesions are frequent occurrences in both human and animal patients, leading to important physiological labor complications that affect the quality of life those who suffer injury. More severe injuries often associated with poor regeneration inadequate functional recovery, even early medical surgical interventions. crush and, therefore, an experimental lesion paradigm widely used researches involving injury models therapies for its resolution. In recent years, many studies...
Bone tissue engineering has been developed in the past decades, with of bone substitutes on vanguard this regenerative approach. Polycaprolactone-based scaffolds are fairly applied for regeneration, and several composites have incorporated so as to improve scaffolds' mechanical properties in-growth. In study, hydroxyapatite is polycaprolactone-based at two different proportions, 80:20 60:40. Scaffolds produced blending methods, solvent casting melt blending. The prepared 3D printed through...
Peripheral nerve injury remains a clinical challenge with severe physiological and functional consequences. Despite the existence of multiple possible therapeutic approaches, until now, there is no consensus regarding advantages each option or best methodology in promoting regeneration. Regenerative medicine promise to overcome this medical limitation, work, chitosan guide conduits olfactory mucosa mesenchymal stem/stromal cells were applied different combinations promote regeneration...
Mesenchymal Stem/ Stromal Cells assume a supporting role to the intrinsic mechanisms of tissue regeneration, feature mostly assigned contents their secretome. A comparative study on metabolomic and bioactive molecules/factors content secretome derived from two expanding sources: umbilical cord stroma dental pulp is presented discussed. The metabolic profile (Nuclear Magnetic Resonance Spectroscopy) evidenced some differences in metabolite dynamics through conditioning period, particularly...
Notwithstanding the advances achieved in last decades field of synthetic bone substitutes, development biodegradable 3D-printed scaffolds with ideal mechanical and biological properties remains an unattained challenge. In present work, a new approach to produce grafts that mimic complex structure is explored. For first time, three various composition, namely polycaprolactone (PCL), PCL/hydroxyapatite nanoparticles (HANp) PCL/HANp/diacrylate poly(ethylene glycol) (PEGDA), were manufactured by...
Central airway obstruction (CAO) represents a pathological condition that can lead to airflow limitation of the trachea, main stem bronchi, bronchus intermedius or lobar bronchus.
Development of synthetic bone substitutes has arisen as a major research interest in the need to find an alternative autologous grafts. Using ovine model, present pre-clinical study presents graft (Bonelike®) combination with cellular system for regeneration non-critical defects. The association biomaterials and cell-based therapies is promising strategy tissue engineering. Mesenchymal stem cells (MSCs) from human dental pulp have demonstrated both vitro vivo interact diverse biomaterial...
Tendon and ligament injuries are frequent in sport horses humans, such represent a significant therapeutic challenge. Tissue regeneration function recovery the paramount goals of tendon lesion management. Nowadays, several regenerative treatments being developed, based on use stem cell cell-based therapies. In present study, preparation equine synovial membrane mesenchymal cells (eSM-MSCs) is described for clinical use, collection, transport, isolation, differentiation, characterization,...
Horses are high-performance athletes prone to sportive injuries such as tendonitis and desmitis. The formation of fibrous tissue in tendon repair remains a challenge overcome. This impels regenerative medicine develop innovative therapies that enhance regeneration, retrieving original properties. Multipotent Mesenchymal Stem/Stromal Cells (MSCs) have been successfully used therapeutic products, they secrete variety bioactive molecules play pivotal role regeneration. These factors released...
Mesenchymal Stromal cells (MSCs) have a potential role in cell-based therapies. Foetal bovine serum (FBS) is used to supplement the basal cell culture medium but presents several disadvantages and risks. Other alternatives been studied, including human umbilical cord blood plasma (hUCBP), aiming at development of xeno-free culturing protocols. A comparative characterization multicomponent metabolic composition hUCBP commercial FBS based on Nuclear Magnetic Resonance (NMR) spectroscopy...
Stem/stromal cell-based therapies are a branch of regenerative medicine and stand as an attractive option to promote the repair damaged or dysfunctional tissues organs. Olfactory mucosa mesenchymal stem/stromal cells have been regarded promising tool in because their several favorable properties such multipotency, high proliferation rate, helpful location, few associated ethical issues. These easily accessible nasal cavity most mammals, including rat, can be applied autologous treatments, do...
Thousands of people worldwide suffer from peripheral nerve injuries and must deal daily with the resulting physiological functional deficits. Recent advances in this field are still insufficient to guarantee adequate outcomes, development new compelling therapeutic options require use valid preclinical models that effectively replicate characteristics challenges associated these humans. In study, we established a sheep model for common peroneal can be applied research advantages large animal...
Bone tissue engineering has been developed in the past decades, with of bone substitutes on vanguard this regenerative approach. PCL based scaffolds are fairly applied for regeneration, and several composites have incorporated, as to improve devices’ mechanical properties ingrowth. In study, HA was incorporated two different proportions, 80:20 60:40. Devices were produced techniques, SC MB, further investigated regards their characteristics vitro cytocompatibility. Results show MB...
Bone injuries represent a major social and financial impairment, commonly requiring surgical intervention due to limited healing capacity of the tissue, particularly regarding critical-sized defects non-union fractures. Regenerative medicine with application bone implants has been developing in past decades towards manufacturing appropriate devices. This work intended evaluate medical 316L stainless steel (SS)-based devices covered by polymer poly (L-lactic acid) (PLLA) coating for lesion...
In this work, dextran-based nerve tube-guides were prepared, characterized and used in a standardized animal model of neurotmesis injury. Non-porous porous transparent obtained by photocrosslinking two co-macromonomers based on dextran poly(ε-caprolactone) (PCL). Swelling capacity the ranged from 40-60% with no visible constriction their inner diameter. vitro hydrolytic degradation tests showed that maintained structural integrity up to 6 months. The vivo performance was evaluated...
In the past decades, regenerative medicine applied on skin lesions has been a field of constant improvement for both human and veterinary medicine. The process healing cutaneous wound injuries implicates well-organized cascade molecular biological processes. However, sometimes normal fails can result in chronic lesion. addition, wounds are considered an increasing clinical impairment, due to progressive ageing population, as well prevalence concomitant diseases, such diabetes obesity, that...
Notwithstanding the advances achieved in last decades field of synthetic bone substitutes, development biodegradable 3D scaffolds with ideal mechanical and biological properties remains an unattained challenge. In this work, a novel approach is explored to produce grafts mimicking complex structure using additive manufacturing. For first time, were produced, extrusion technique, composed thermoplastic polymer, polycaprolactone (PCL), hydroxyapatite nanoparticles (HANp), polyethylene glycol...
Tendons and ligaments injuries are frequent in sport horses humans, representing a great therapeutic challenge. Tissue regeneration function recovery the paramount of tendon ligament lesions management. Nowadays, several regenerative treatments being developed, based on use stem cell cell-based therapies. In present study, preparation equine synovial membrane mesenchymal cells (eSM-MSCs) for clinical - collection, transport, isolation, differentiation, characterization, application is...
Autologous bone remains the gold standard grafting substrate for fusions used small gaps and critical defects. However, significant morbidity is associated with harvesting of autologous grafts and, that reason, alternative graft substitutes have been developed. In present case series, a glass-reinforced hydroxyapatite synthetic substitute, osteoinductive osteoconductive proprieties, was applied. This substitute comprises incorporation P2O5-CaO glass-based system within matrix, moulded into...