A5074661249- Photosynthetic Processes and Mechanisms
- Cancer, Hypoxia, and Metabolism
- Plant-Microbe Interactions and Immunity
- Mitochondrial Function and Pathology
- Tryptophan and brain disorders
- RNA modifications and cancer
- Biochemical and Molecular Research
- Mycorrhizal Fungi and Plant Interactions
- Plant tissue culture and regeneration
- Bacteriophages and microbial interactions
- Plant Reproductive Biology
- Lipid metabolism and biosynthesis
- Biochemical Analysis and Sensing Techniques
- Adipokines, Inflammation, and Metabolic Diseases
- Metabolomics and Mass Spectrometry Studies
- Plant Gene Expression Analysis
- Plant Parasitism and Resistance
- Plant responses to water stress
- Neuroinflammation and Neurodegeneration Mechanisms
- Algal biology and biofuel production
- Cancer Research and Treatments
- Advances in Cucurbitaceae Research
- Stress Responses and Cortisol
- Bacterial biofilms and quorum sensing
- Potato Plant Research
Weizmann Institute of Science
2011-2025
Agricultural Research Organization
2012-2023
Planta
2022
Kaplan Medical Center
2021
From Nasty to Tasty Some of our favorite food crops derive from wild relatives that were distasteful or even toxic. Domestication over many years selected for variants with reduced levels antinutritional compounds. The remain valuable, however, other traits such as resistance pathogens, but their use in crop development is complicated by the continued presence unpalatable Itkin et al. (p. 175 , published online 20 June) elucidate metabolic pathways and genes directing synthesis some these...
After fertilization, the expanding carpel of fleshy fruit goes through a phase change to ripening. Although role ethylene signalling in mediating climacteric ripening has been established, knowledge regarding regulation biosynthesis and its association with developmental programs is still lacking. A functional screen tomato transcription factors showed that silencing TOMATO AGAMOUS-LIKE 1 (TAGL1) MADS box gene results altered pigmentation. Over-expressing TAGL1 as chimeric repressor...
Abstract Steroidal alkaloids (SAs) are triterpene-derived specialized metabolites found in members of the Solanaceae family that provide plants with a chemical barrier against broad range pathogens. Their biosynthesis involves action glycosyltransferases to form steroidal glycoalkaloids (SGAs). To elucidate metabolism SGAs family, we examined tomato (Solanum lycopersicum) GLYCOALKALOID METABOLISM1 (GAME1) gene. Our findings imply GAME1 is galactosyltransferase, largely performing...
Abstract Ferroptosis and apoptosis are key cell‐death pathways implicated in several human diseases including cancer. is driven by iron‐dependent lipid peroxidation currently has no characteristic biomarkers or gene signatures. Here a continuous phenotypic gradient between ferroptosis coupled to transcriptomic metabolomic landscapes established. The gradual ferroptosis‐to‐apoptosis landscape used generate unique, unbiased predictor, the Gradient Gene Set (GGS), which classified with high...
Caspase family proteases and Toll/interleukin-1 receptor (TIR)-domain proteins have central roles in innate immunity regulated cell death humans. We describe a bacterial immune system comprising both caspase-like protease TIR-domain protein. found that the TIR protein, once it recognizes phage invasion, produces previously unknown signaling molecule adenosine 5′-diphosphate-cyclo[N7:1′′]-ribose (N7-cADPR). This specifically activates protease, which then indiscriminately degrades cellular to...
Significance We identified the biosynthetic pathway for nonsugar sweetener mogroside V, a noncaloric with sweetening strength 250-fold that of sucrose. This compound is produced by fruit endemic Chinese cucurbit Siraitia grosvenoriii , also known as monk and luo-han-guo . The metabolic was using combination genomic transcriptomic databases plant, together large-scale functional expression candidate genes. novelty could be attributed to highly coordinated gene pattern responsible unique...
Key to the success of intracellular pathogens is ability sense and respond a changing host cell environment. Macrophages exposed microbial products undergo metabolic changes that drive inflammatory responses. However, role macrophage reprogramming in bacterial adaptation environment has not been explored. Here, using profiling dual RNA sequencing, we show succinate accumulation macrophages sensed by Salmonella Typhimurium (S. Tm) promote antimicrobial resistance type III secretion. S Tm...
Microbial communities employ a variety of complex strategies to compete successfully against competitors sharing their niche, with antibiotic production being common strategy aggression. Here, by systematic evaluation four non-ribosomal peptides/polyketide (NRPs/PKS) antibiotics produced Bacillus subtilis clade, we revealed that they acted synergistically effectively eliminate phylogenetically distinct competitors. The these came fitness cost manifested in growth inhibition, rendering...
Abstract Systemic immunity supports lifelong brain function. Obesity posits a chronic burden on systemic immunity. Independently, obesity was shown as risk factor for Alzheimer’s disease (AD). Here we show that high-fat obesogenic diet accelerated recognition-memory impairment in an AD mouse model (5xFAD). In obese 5xFAD mice, hippocampal cells displayed only minor diet-related transcriptional changes, whereas the splenic immune landscape exhibited aging-like CD4 + T-cell deregulation....
Root exudates are thought to play an important role in plant-microbial interactions. In return for nutrition, soil bacteria can increase the bioavailability of nutrients. However, root typically decrease situations such as drought, calling into question efficacy solvation and bacteria-dependent mineral uptake stress. Here, we tested hypothesis exudate-driven microbial priming on Cupressus saplings grown forest custom-made rhizotron boxes. A 1-month imposed drought concomitant inoculations...
Electrophiles for covalent inhibitors that are suitable in vivo administration rare. While acrylamides prevalent FDA-approved drugs, chloroacetamides considered too reactive such purposes. We report sulfamate-based electrophiles maintain chloroacetamide-like geometry with tunable reactivity. In the context of BTK inhibitor ibrutinib, sulfamate analogues showed low reactivity comparable potency protein labeling, vitro, and cellular kinase activity assays were effective a mouse model CLL....
Abstract Bacteria defend against phage infection via a variety of antiphage defense systems. Many systems were recently shown to deplete cellular nicotinamide adenine dinucleotide (NAD + ) in response infection, by breaking NAD ADP-ribose (ADPR) and nicotinamide. It was demonstrated that depletion during deprives the from this essential molecule impedes replication. Here we show substantial fraction phages possess enzymatic pathways allowing reconstitution its degradation products infected...
Gut microbiota have been shown to promote oogenesis and fecundity, but the mechanistic basis of remote influence on remained unknown. Here, we report a systemic mechanism mediated by bacterial-derived supply mitochondrial coenzymes. Removal decreased activity ATP levels in whole-body ovary, resulting repressed oogenesis. Similar repression was caused RNA-based knockdown function ovarian follicle cells. Reduced germ-free (GF) females reversed bacterial recolonization or supplementation...
Significance Studies in mice and humans have revealed daytime variance exercise performance, yet it is still unclear whether how these differences are regulated by the circadian clock. Here, we show that performance clock controlled specific proteins modify capacity a time-dependent manner, likely through food consumption liver glycogen stores. We also demonstrate time of day at which training performed plays role improving capacity. Our study suggests shaped daytime-dependent manner.
Abstract In higher plants, the plastidial NADH dehydrogenase (Ndh) complex supports nonphotochemical electron fluxes from stromal donors to plastoquinones. Ndh functions in chloroplasts are not clearly established; however, its activity was linked prevention of overreduction stroma, especially under stress conditions. Here, we show by characterization OrrDs, a dominant transposon-tagged tomato (Solanum lycopersicum) mutant deficient NDH-M subunit, that this is also essential for fruit...