Aberrant activation of the Wnt/β-catenin signaling pathway is frequently observed in colorectal cancer (CRC). β-catenin major Wnt effector and inactivation adenomatous polyposis coli (APC) results nuclear accumulation β-catenin. It has been suggested that APC plays an important role progression tumorigenesis. However, mechanism through which mediates tumorigenesis not understood. Increasing evidence suggests dysregulation microRNAs (miRNAs) involved Although miR-494 reported as being...

Unique change of circulating microRNAs (miRNAs) was recognized to occur in early oncogenesis, which conferred it the potential as biomarkers for detection cancer. However, its diagnostic capability hepatocellular carcinoma (HCC) has not been fully understood. In this study, microarray analysis applied screen initial candidate miRNA from both supernatants anoikis-resistant cellular models and sera samples HCC patients. The selected differentially expressed miRNAs were further verified 115...

Background: Several studies have confirmed the role of microRNAs in regulating ischemia/reperfusion-induced cardiac injury (I/R-I). MiR-17-5p has been regarded as an oncomiR development cancer. However, its potential cardiomyocytes not exploited. The aim this study is to investigate miR-17-5p I/R-I and underlying mechanism through targeting Stat3, a key surviving factor cardiomyocytes. Methods: MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5 diphenyl tetrazolium bromide) assay was used detect cell...

Abstract BACKGROUND: Distinct morphologic features of microvascular endothelium exist in tumor tissues. The objective this study was to investigate the prognostic value endothelium‐coated clusters (ECTCs) hepatocellular carcinoma (HCC). METHODS: ECTCs were evaluated by immunohistochemical staining for cluster differentiation 34 (CD34) (a cell surface glycoprotein which is expressed specifically on HCC) 239 specimens from patients with primary HCC. Overall survival (OS) and time recurrence...

Breastfeeding has been shown to have a protective effect on the occurrence of necrotizing enterocolitis (NEC), but mechanism remains unclear. In context NEC pathogenesis, many properties exosomes intestinal epithelial compartment make it an ideal therapeutic target. present study, our hypothesis was that stem cells (ISCs) would be protected from injury by human milk-derived (HMDEs). Human breast milk collected, and were isolated using ExoQuick reagent. Magnetic-activated cell sorting...

Studies have shown that cancer susceptibility candidate 11 (CASC11), a newly discovered long non-coding RNA (lncRNA), was aberrantly overexpressed in hepatic carcinoma, gastric and colorectal cancer. However, its effects on cervical has been kept unknown up to now. The present study aimed investigate the relationship between lncRNA CASC11 further explore mechanism of effect progression. Quantitative real-time polymerase chain reaction (RT-qPCR) used detect expressions cancerous adjacent...

BACKGROUND: Studies have shown that lncRNA nuclear paraspeckle assembly transcript 1 (NEAT1) is related to breast cancer progression, however, the role of NEAT1 remains largely unknown. The aim current study was further investigate function involved in cancer. METHODS: QRT-PCR used analyze expression tissues and determine association between clinicopathologic features patients. Kaplan-Meier analysis log-rank test were establish relationship overall survival. Cell proliferation invasion...

Curcumin is a key polyphenolic curcuminoid extracted from the root of turmeric rhizome Curcuma longa Linn, which frequently used Chinese herb for treatment cancer. The aim present study was to investigate mechanism inhibitory effects curcumin on nude mice with lung cancer A549 cell subsets side population (SP) and non-SP (NSP) cells. BALB/c were subcutaneously injected tumor cells SP or NSP consisting 1×109 cells/l (0.2 ml in total). After 16 days inoculation A549, intraperitoneally (100...

BARLEY B-RECOMBINANT/BASIC PENTACYSTEINE (BBR/BPC), a plant-specific transcription factor family, is group of GAGA_motif binding factors controlling multiple developmental processes growth and response to abiotic stresses. BPCs recruit histone remodeling for transcriptional repression downstream targets. However, the information about BnaBPCs from Brassica napus remains unclear. Here, we identified 25 BnaBPC genes that were mainly localized in nucleus, randomly on 16 chromosomes, grouped...

Although previous studies have demonstrated that dysregulation of microRNA (miR)-126 is associated with the progression several types cancer, including lung relationship between miR-126 and cancer metastasis remains unclear. SPC-A1 cells were transfected mimic negative control using Lipofectamine® 3000. Following 2 h, TGF-β1 was used to induce epithelial-to-mesenchymal transition (EMT). The protein expression levels EMT markers: E-cadherin, fibronectin, N-cadherin vimentin detected by...

Helsmoortel-Van der Aa syndrome (HVDAS) is a rare genetic disorder caused by variants in the activity-dependent neuroprotector homeobox (ADNP) gene; hence, it also called ADNP syndrome. multitasking protein with function as transcription factor, playing critical role brain development. Furthermore, have been identified one of most common single-gene causes autism spectrum (ASD) and intellectual disability. We assembled cohort 15 Chinese pediatric patients, 13 coding region gene, evaluated...

The aim of this study was to investigate the role microRNAs (miRNAs or miRs) in vascular smooth muscle cell (VSMC) proliferation and elucidate underlying molecular mechanisms. In a previous study, using microarray analysis, differentially expressed miRNAs were identified primary VSMCs isolated from medial layer thoracic aorta obtained spontaneously hypertensive rats (SHRs) Wistar Kyoto (WKY) rats. Among others, miR-1 be downregulated SHRs. Thus, present we focused on miR-1, downregulation...

Purpose: To investigate the effect of alprostadil on myocardial ischemia/reperfusion (I/R) in rats. Methods: Rats were subjected to ischemia for 30 min followed by 24h reperfusion. Alprostadil (4 or 8 μg/kg) was intravenously administered at time reperfusion and infarct size, levels troponin T, activity creatine kinase-MB (CK-MB) lactate dehydrogenase (LDH) serum measured. Antioxidative parameters, nitric oxide (NO) content phosphorylated endothelial synthase 3 (p-eNOS) expression left...

There is a large amount of evidence that anti-angiogenic drugs are effective safe. However, few studies have evaluated the specific effects on myocardial enzyme injury biomarkers: aspartate aminotransferase (AST), lactic dehydrogenase (LDH), creatine kinase (CK) and isoenzyme (CK-MB). The purpose our study was to determine whether serum AST, LDH, CK, CK-MB activities cancer patients treated with drugs.

Colorectal cancer has become a disease with one of the highest incidence rates worldwide, and liver metastasis threatens prognosis colorectal cancer. MicroRNA (miR)‑200 miR‑141 have been confirmed to be aberrantly expressed in cancer, however, whether miR‑200 are involved progression their role remain fully elucidated. The aim present study was investigate patients. A total 380 patients were enrolled, which 142 diagnosed grade I/II 238 III/IV disease. Reverse transcription‑quantitative...

// Ying Zhang 1 , Linpei Jia 2 3 Wei Ji 4 and Hai Li 5 Department of Pathology, China-Japan Union Hospital Jilin University, Changchun 130033, P.R. China Nephrology, Xuanwu Hospital, Capital Medical Beijing 100000, Neurology, First 130021, Vascular Surgery, Provincial People’s 130000, Urology, Correspondence to: Li, email: muandkamu_2012@aliyun.com Keywords: bladder cancer; forkhead box class O; clinicopathological characteristics; prognosis Received: August 30,...

Colorectal cancer (CRC), a common malignancy worldwide, and microRNAs (miRs) have been suggested to play roles in the disease. MiR-566 expression has shown be reduced CRC, but its functions mechanisms are still unclear.Cell viability was assessed by using CellTiter 96 AQueous One Solution Cell Proliferation kit. proliferation measured with MTT assay. metastasis were transwell Luciferase reporter assays used confirm target of MiR-566. PSKH1 RT-PCR western blot.In present study, we first...