A5101847665- Ion channel regulation and function
- Ion Transport and Channel Regulation
- Cancer-related molecular mechanisms research
- Neuroscience and Neuropharmacology Research
- Multiple Myeloma Research and Treatments
- Circular RNAs in diseases
- MicroRNA in disease regulation
- Spinal Cord Injury Research
- Redox biology and oxidative stress
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Cardiac electrophysiology and arrhythmias
- ATP Synthase and ATPases Research
- Ginseng Biological Effects and Applications
- Natural product bioactivities and synthesis
- Chronic Myeloid Leukemia Treatments
- Chronic Kidney Disease and Diabetes
- Cancer, Hypoxia, and Metabolism
- Mitochondrial Function and Pathology
- Spectroscopy and Quantum Chemical Studies
- Heat shock proteins research
- Biomarkers in Disease Mechanisms
- Bariatric Surgery and Outcomes
- Body Contouring and Surgery
- Neurogenesis and neuroplasticity mechanisms
- TGF-β signaling in diseases
Center for Life Sciences
2020-2024
Tsinghua University
2020-2024
Sanofi (United States)
2015-2020
Union Hospital
2014-2019
Jilin University
2009-2019
Second Affiliated Hospital of Xi'an Jiaotong University
2019
Union Hospital
2018-2019
Hunan University of Traditional Chinese Medicine
2018
Nanjing Medical University
2017
Jiangsu Province Hospital
2017
The aim of the present study was to investigate potential role regulated in development and DNA damage response 1 (REDD1) LPS-induced vascular endothelial injury by using human umbilical vein cells (HUVECs). We observed that REDD1 expression apparently elevated HUVECs after exposure LPS. Additionally, elimination strikingly attenuated secretion proinflammatory cytokines TNF-α, IL-6, IL-1β, monocyte chemotactic protein-1 cell adhesion markers ICAM-1 VCAM-1 induced LPS stimulation....
The translocase of the outer mitochondrial membrane (TOM) complex is main entry gate for precursor proteins synthesized on cytosolic ribosomes. Here we report single-particle cryo-electron microscopy (cryo-EM) structure dimeric human TOM core (TOM-CC). Two Tom40 β-barrel proteins, connected by two Tom22 receptor subunits and one phospholipid, form protein-conducting channels. small Tom Tom5, Tom6, Tom7 surround channel have notable configurations. distinct electrostatic features complex,...
<b><i>Background/Aims:</i></b> Endothelial-to-mesenchymal transition (EndMT) of glomerular endothelial cells (GEnCs) can induce albuminuria in diabetic nephropathy. Melatonin attenuates nephropathy, but its role and mechanism EndMT GEnCs nephropathy remain unknown. <b><i>Methods:</i></b> The effect melatonin on induced by transforming growth factor (TGF)-β2 human renal was determined assaying the expression marker (VE-cadherin CD31) mesenchymal...
Abstract microRNAs (miRNAs) have been revealed to participate in the pathological process of atherosclerosis (AS). However, exact role miR‐338‐3p, a target miRNA BMP and activin membrane‐bound inhibitor (BAMBI), its possible molecular mechanism AS remain unidentified. In this study, we found that BAMBI was significantly decreased, whereas miR‐338‐3p increased patients with oxidized low‐density lipoprotein (ox‐LDL)‐induced HUVEC cells. Furthermore, overexpression decreased cell viability...
ClC chloride channels possess a homodimeric structure in which each monomer contains an independent ion pathway. channel gating is regulated by concentration, pH and voltage. Based on structural physiological evidence, it has been proposed that glutamate residue the extracellular end of selectivity filter acts as fast gate. We utilized new search algorithm incorporates electrostatic information to explore transit pathways through wild-type mutant bacterial channels. Examination permeation...
Background/Aims: Endogenous parathyroid hormone (PTH) plays an important role in fracture healing. This study investigated whether endogenous PTH regulates healing by bone morphogenetic protein (BMP) and/or the transforming growth factor-β (TGF-β) signaling pathway. Methods: Eight-week-old wild-type (WT) and PTH-knockout (PTH KO) male mice were selected, models of open right-femoral constructed. Fracture callus characteristics two groups compared X-ray, micro-computed tomography,...
Polyamines are important polycations that play critical roles in mammalian cells. ATP13A2 belongs to the orphan P5B adenosine triphosphatases (ATPase) family and has been established as a lysosomal polyamine exporter maintain normal function of lysosomes mitochondria. Previous studies have reported several human neurodegenerative disorders related mutations gene. However, transport mechanism lysosome remains unclear. Here, we report cryo-electron microscopy (cryo-EM) structures three...
Chronic intermittent hypobaric hypoxia (CIHH) has been shown to attenuate intracellular Na(+) accumulation and Ca(2+) overload during ischemia reperfusion (I/R), both of which are closely related the outcome myocardial damage. Na/K pump plays an essential role in maintaining equilibrium I/R. It that enhancement activity by ischemic preconditioning may be involved cardiac protection. Therefore, we tested whether was cardioprotection CIHH. We found current myocytes guinea pigs exposed CIHH...
Buyang huanwu decoction (BYHWD) is a classic recipe in traditional Chinese medicine (TCM) to supplement Qi and activate blood. It has been used recover the neural function after injury of central nervous system for hundreds years China.This study investigated whether combined with bone marrow mesenchymal stem cells (BMSCs) transplantation had synergistic effect on neuroprotection red nucleus neurons spinal cord (SCI).Rubrospinal tract (RST) transection model was established BMSCs were...
Colorectal cancer (CRC) is a common disease with high mortality and morbidity. Annexin A3 (ANXA3) belongs to the structurally homologous family of Ca2+ phospholipid-binding proteins. This study aimed investigate effects potential mechanisms ANXA3 on oxaliplatin (Ox) resistance in CRC. We generated two human CRC cell lines (HCT116/Ox SW480/Ox) acquired Ox determined their properties. expression apoptosis, migration invasion also were evaluated. found that viability HCT116/Ox SW480/Ox was...
Abstract Na + /K ‐ATPase and N ‐methyl‐ d ‐aspartate (NMDA) receptor in hippocampus play very important roles the regulation of learning memory. Here, we showed that dihydroouabain (DHO, 10 −5 –10 −3 m ), a inhibitor, significantly potentiated NMDA current rat hippocampal CA1 pyramidal neurons, which was blocked by PP2 (the selective Src tyrosine kinase inhibitor) PD‐98059 [the inhibitor mitogen‐activated protein kinases (MAPK) cascade]. These findings reported here uncover mediates...
Colorectal cancer (CRC), a common malignancy worldwide, and microRNAs (miRs) have been suggested to play roles in the disease. MiR-566 expression has shown be reduced CRC, but its functions mechanisms are still unclear.Cell viability was assessed by using CellTiter 96 AQueous One Solution Cell Proliferation kit. proliferation measured with MTT assay. metastasis were transwell Luciferase reporter assays used confirm target of MiR-566. PSKH1 RT-PCR western blot.In present study, we first...
Background: Many stressful conditions, including cardiovascular diseases, induce long-term elevations in circulating catecholamines, thereby leading to changes of the Na/K pump and thus affecting myocardial functions. However, only short-term adrenergic regulation has been reported. The present study is first investigation potential mechanism. Methods: After acutely isolated Sprague-Dawley rat myocytes were incubated with noradrenaline or isoprenaline for 24 h, high- (IPH) low-affinity...