Circular RNAs (circRNAs) are a class of non-coding broadly expressed in cells various species. However, the molecular mechanisms that link circRNAs with colorectal cancer (CRC) not well understood. In present study, we attempted to provide novel basis for targeted therapy CRC from aspect circRNA-microRNA (miRNA)-mRNA interaction.We investigated expression five paired tissues and adjacent non-tumor by microarray analysis. Differentially were identified between non-cancerous matched tissues....

miRNAs are a class of endogenous non-coding RNA, which can regulate downstream target genes through binding to the 3'UTR those genes. Numerous studies have indicated that abnormal expression is implicated in tumor development. Aberrant miR-31 has been found various cancers, including colorectal cancer. Here, we show upregulated human colon cancer tissues and cell lines, repression inhibited proliferation colony formation soft agarose. To further elucidate mechanism underlying role promoting...

<b><i>Background/Aims:</i></b> Endothelial-to-mesenchymal transition (EndMT) of glomerular endothelial cells (GEnCs) can induce albuminuria in diabetic nephropathy. Melatonin attenuates nephropathy, but its role and mechanism EndMT GEnCs nephropathy remain unknown. <b><i>Methods:</i></b> The effect melatonin on induced by transforming growth factor (TGF)-β2 human renal was determined assaying the expression marker (VE-cadherin CD31) mesenchymal...

Gastric cancer (GC) is an aggressive tumor featured by uncontrolled cell proliferation and metastasis. In recent years, long noncoding RNAs (lncRNAs) act as crucial regulators biological markers in multiple cancers. LncRNA TMPO-AS1 has been revealed to be oncogene some Nevertheless, there little known about the role of GC.Reverse transcription-quantitative polymerase chain reaction analysis was used examine expression level GC tissues cells. Cell Counting Kit-8, colony formation, wound...

ABSTRACT Gastric cancer (GC) represents a major global health concern, with over 1 million new cases diagnosed annually worldwide. Emerging studies have highlighted the significant correlation between long noncoding RNAs (lncRNAs) and progression of GC. The objective current study is to investigate roles mechanism lncRNA homeobox A10 antisense RNA (HOXA10‐AS) in modulating malignant properties GC cells. RT‐qPCR was employed detect HOXA10‐AS expression cells or human normal gastric epithelium...

Background: Membranous glomerulonephritis (MGN) is a nephrotic syndrome which shows the symptoms of heavy proteinuria and immune complex deposition in glomerular sub-epithelial space finally leads to chronic kidney disease. Isoliquiritin (ILQ) flavonoid with wide range pharmacological properties, including antioxidant anti-inflammatory activity. The present study was undertaken investigate possible mechanisms by ILQ ameliorates cationic bovine serum albumin (C-BSA) induced MGN rat model....

Colorectal cancer (CRC), characterized by high mortality and incidence rate, is one of the most common types rectum tumors in gastrointestinal tract worldwide. An increasing number investigations indicated that long noncoding RNAs (lncRNAs) have been implicated growth a wide range cancers. Although it has obtained general acceptance lncRNA LINC00511 plays significant role numerous cancers, regulatory mechanism CRC still needs to be explored.Bioinformatics analysis experiments sphere...

Colorectal cancer (CRC) is a common disease with high mortality and morbidity. Annexin A3 (ANXA3) belongs to the structurally homologous family of Ca2+ phospholipid-binding proteins. This study aimed investigate effects potential mechanisms ANXA3 on oxaliplatin (Ox) resistance in CRC. We generated two human CRC cell lines (HCT116/Ox SW480/Ox) acquired Ox determined their properties. expression apoptosis, migration invasion also were evaluated. found that viability HCT116/Ox SW480/Ox was...

Colorectal cancer (CRC), a common malignancy worldwide, and microRNAs (miRs) have been suggested to play roles in the disease. MiR-566 expression has shown be reduced CRC, but its functions mechanisms are still unclear.Cell viability was assessed by using CellTiter 96 AQueous One Solution Cell Proliferation kit. proliferation measured with MTT assay. metastasis were transwell Luciferase reporter assays used confirm target of MiR-566. PSKH1 RT-PCR western blot.In present study, we first...

Objective To observe the effects of exogenous expression VEGF165b on viability, migration rate and invasion ability human bladder cancer T24 cells.and to explore influence in biological constructed. The cells were divided into group (control), pcDNA3.0 (transfected by plasmid) pc DNA-VEGF165b pcDNA-VEGF165b plasmid). viability cell was detected MTT assay; Western blotting method used determine expressions VEGF165b, matrix metalloproteinase-2 (MMP-2) metalloproteinase-9 (MMP-9) proteins....

To study the clinical effect of glucocorticoid combined with thalidomide in treatment refractory nephrotic syndrome membranous nephropathy. Methods: pathological for nephropathy 26 patients syndrome, + gilad amine 50 mg 2 times/d, to turn urine protein application again after 12 weeks reduction mg, l/d, maintain half year outage. The invalid was given a maximum 24 stop drug. observation time weeks. Results: 24h decreased from 9.15 ±3.23 1.43±1.91 (P<0.01).Plasma albumin rose 26.02 ± 6.75...

Small lesion is a common pathological type nephrotic syndrome, especially in high-risk children; complications include infection, thrombosis, and acute renal failure protein lipid metabolism disorders. The pathogenesis of this disease still not clear, the current research focus has been turned to study foot cells by T cell immune However, CD80 expressed small glomerular lesions, related formation egg white urine. This article reviewsthe relationship between micropathy.

Systemic lupus erythematosus (SLE) is a prototypical systemic autoimmune disease, characterized by wide array of symptoms and organ involvements, leading to varying disease courses outcome, ranging from mild severe types. In patients with SLE, the incidence risk malignancy development increased, non-Hodgkin's lymphoma (NHL), cervical cancer, as well bronchial carcinomas occur, are common Common genetic predisposition, chronic antigen stimulus, disproportional immune responses, administration...

Studies have shown that diabetic feet occur in 15%~25% diabetics, and about half of them will wound infection. There are many factors lead to poor prognosis foot, such as peripheral vascular disease, area depth foot ulcer, neuropathy Therefore, the pathogenesis is complex, one main causes leading amputation. At same time, ulcers can significantly increase health risk patients; incidence cardiovascular accidents all-cause mortality. early diagnosis treatment an important means improve quality...

IgA nephropathy is the most common primary glomerular disease in world, accounting for about 25% ~50% of diseases [1]. Clinical occurs young and middle-aged adults, 30-40% patients enter end-stage renal failure (ESRD) 10-20 years[2], which brings a heavy burden to country society. pathogenesis not clear at present, has long been mesangial cells considered be major cell types disease, recent study found that sertoli interaction between tubular epithelial cells, cause kidney damage [3-5]. 2009...

Objective to investigate the efficacy and clinical application of catheter thrombolysis in treatment acute deep venous thrombosis lower extremities. Patients with limb DVT 2016 November 2013 year 50 cases as experimental group, June 2007 2010 December were extremity patients 69 control group. All diagnosed by venography or Doppler intravenous ultrasound, symptoms such pain tenderness groin area, so on. Results curative effect 12 groups test group cases, 1 lost, 13 cured, 34 markedly...

To investigate the effect and safety of inferior vena cava filter (VCF) implantation in prevention secondary pulmonary embolism (DVT) lower extremity deep venous thrombosis (DVT). Methods: observe 225 patients received VCF combined with thrombolytic therapy, clinical efficacy complications simple catheter directed thrombolysis systemic treatment DVT incidence, monitoring oxygen saturation (Sp O2) arterial pressure (Pa, were (PE) occurrence recurrent after treatment. Results: difference...

To observe the curative effect of slow infusion low-dose urokinase and low molecular weight heparin calcium in elderly patients with acute deep venous thrombosis. Methods: 45 thrombosis lower extremities were selected randomly divided into two groups. 20 cases control group using traditional method administration by limbs other body superficial vein small dose (400000uꞏtimes-1,1timeꞏd -1) thrombolysis (100uꞏkg-1, Ih, 1timeꞏ12 h-1) on first day anticoagulation. Play warfarin 2.5 mg - late 1...

Gastric cancer is a common malignant tumor of the digestive system with no specific symptoms. Due to limited knowledge pathogenesis, patients are usually diagnosed in advanced stage and do not have effective treatment methods. Proteome has unique tissue time specificity can reflect influence external factors that become potential biomarker for early diagnosis. Therefore, discovering gastric cancer-related proteins could greatly help researchers design drugs develop an diagnosis kit. However,...