Dendrobium officinale, an important medicinal plant of the genus in Orchidaceae family, has been used as traditional Chinese medicine (TCM) for nearly thousands years. Here, we report first chromosome-level reference genome D. based on PacBio long-reads, Illumina short-reads and Hi-C data. The high-quality assembled is 1.23 Gb long, with contig N50 1.44 Mb. A total 93.53% sequences were into 19 pseudochromosomes a super scaffold 63.07 Through comparative genomic analysis, explored expanded...

Mitochondrial genomes are essential for deciphering the unique evolutionary history of seed plants. However, rules their extreme variation in genomic size, multi-chromosomal structure, and foreign sequences remain unresolved most plant lineages, which further hindered application mitogenomes phylogenetic analyses.Here, we took Dendrobium (Orchidaceae) shows great divergence morphology difficulty species taxonomy as study focus. We first de novo assembled two complete wilsonii henanense that...

The global prevalence of herbal-based health care rapidly promoted requirements for medicinal plant resources. Accurate classification and identification are crucial to assuring the safety these herbal sources. Here, we took Dendrobium (Orchidaceae), a famous horticultural taxon, as study focus establish an effective authentication approach plants based on new mtDNA barcodes. We first de novo assembled three complete mitogenomes using Illumina Nanopore data. These were 635,454 bp-831,745 bp...

Inflammation is a critical component of tumor progression, and it modifies the microenvironment by various mechanisms. Here, we explore effect inflammatory response on in colorectal cancer (CRC). A prognostic signature consisting inflammation-related genes (IRGs) was constructed verified based bioinformatics analysis. IRG risk model identified as an independent factor CRC, related to biological processes extracellular matrix, cell adhesion angiogenesis. The score predicted clinical benefit...

Abstract Proteases secreted by pathogens have been shown to be important virulence factors modifying plant immunity, and cysteine proteases demonstrated participate in different pathosystems. However, the functions of Phytophthora parasitica are poorly understood. Using a publicly available genome database, we identified 80 P. , 21 which were secreted. Most conserved among strains induced during infection. The protease proteins PpCys44/45 (proteases with identical protein sequences) PpCys69...

Dendrobium orchids have multiple photosynthetic pathways, which can be used as a model system for studying the evolution of crassulacean acid metabolism (CAM). In this study, based on results net rates (Pn), we classified species into three then employed and compared their chloroplast genomes. The genomes typical quartile structures, ranging from 150,841-153,038 bp. apparent differences in GC content, sequence variability, IR junctions SSC/IRB (JSBs) were measured within among different...

Abstract Reconstructing mitochondrial genomes of angiosperms is extremely intricate due to frequent recombinations which give rise varied sized in Dendrobium mitogenomes and their structural variations, even most orchid species. In this study, we first sequenced two complete five draft using next‐generation third‐generation sequencing technologies. The were 420 538–689 048 bp long, showing multipartite (multichromosomal) structures that consisted variably circular or linear‐mapping isoforms...

Inherited susceptibility and environmental carcinogens are crucial players in lung cancer etiology, both exhibit population heterogeneity. MUC16 is overexpressed various cancers often associated with poor prognosis. Present work was to investigate the clinical significance of non-small cell patients affected by familial (FLC) indoor air pollution caused coal use.Clinicopathologic characteristics expression were analyzed evaluated our subject population. Vectors constructed for gene knockout...

Because chloroplast (cp) genome has more conserved structures than nuclear and mitochondrial genome, it is a useful tool in estimating the phylogenetic relationships of plants. With series researches for cp genomes, there have been comprehensive understandings about features. The genus Bulbophyllum widely distributed Asia, South America, Australia other places. Therefore, an excellent type studying effects geographic isolation.In this study, genomes nine orchids were newly sequenced...

Abstract Background Cuproptosis, a new form of programmed cell death, has been recently reported to be closely related tumor progression. However, the significance cuproptosis-related genes (CRGs) in papillary thyroid carcinoma (PTC) is still unclear. Therefore, this study aimed investigate role CRG signature prognosis prediction and immunotherapeutic effect estimation patients with PTC. Methods RNA-seq data corresponding clinical information PTC were obtained from Cancer Genome Atlas (TCGA)...

Cadherin EGF LAG seven-pass G-type receptor 3 (CELSR3) has been reported in cancers but its role and potential molecular mechanism hepatocellular carcinoma (HCC) is unclear. Therefore, we aimed to investigate the clinical value of CELSR3 HCC using an vitro experiment, a meta-analysis bioinformatics. The experiment determined promoting effect proliferation, invasion, migration cells. knockout causes S-phage arrest can also inhibit apoptosis expression gene protein was significantly elevated...

Dendrobium officinale Kimura et Migo, which contains rich polysaccharides, flavonoids and alkaloids, is a Traditional Chinese Medicine (TCM) with important economic benefits, while various pathogens have brought huge losses to its industrialization. NBS gene family the largest class of plant disease resistance (R) genes, proteins are widely distributed in upstream downstream immune systems responsible for receiving infection signals regulating expression respectively. It great significance...

Cuticular proteins (CPs) are major components of the insect cuticle-associated organs such as integument and wings, although importance CPs for wing development function in hemimetabolous insects remains understudied. In present study, a cuticular protein LmACP8 was identified from Locusta migratoria, which belongs to RR-2 subfamily R&R consensus (CPR) chitin-binding proteins. mainly expressed pads showed high expression levels before ecdysis third-, fourth-, fifth-instar nymphs, with its...

Dendrobium brymerianum Rchb. f. is a species of orchid with pharmacological interest for its potential to inhibit the growth human lung cancer cells. The identification notable problem due morphological similarities and limitations universal DNA barcodes. To overcome these difficulties, this study employed complete chloroplast (cp) genome sequences as useful resources D. brymerianum. Based on Illumina sequencing, cp genomes five individuals were assembled. These in quadripartite structure,...

The aim of this study was to investigate the expression PTEN and PIK3CA in pemetrexed-resistant human pancreatic cancer cell line Patu8988, evaluate their effects on biological behavior cells.PTEN gene protein expressions were detected by reverse transcriptase polymerase chain reaction (RT-PCR) western blot, respectively, a parent strain cells.The discrepancies between two types lines transwell test.RT-PCR blot analyses revealed that overexpressed line.PTEN shown be upregulated 89 76%...

Bulbophyllum disciflorum is one of Orchidaceae species, which has important ornamental and economic value. Here, we reported the first chloroplast genome sequence Bulbophyllum. The B. 148,554 bp in length, including a large single-copy (LSC) 79,001 bp, small (SSC) 16,797 pair inverted repeat regions (IRa IRb) 26,378bp. It contains 108 unique genes consisting 74 protein-coding genes, 30 tRNA 4 rRNA genes. phylogenetic analysis revealed that sister to genus Dendrobium.

Oxystophyllum changjiangense has high economic value due to its wide applications in horticultural and medicinal fields. Here, the first chloroplast genome of O. was sequenced reported. The displayed typical quadripartite structure containing a pair inverted repeats (IR), long single-copy region (LSC), short (SSC). Total 110 genes were found including 76 protein-coding genes, 30 tRNA four rRNA genes. Phylogeny analysis showed close relationship with Phaius species.

Dendrobium orchids, which are among the most well-known species of appreciated for their aesthetic appeal across globe. Furthermore, due to strict living conditions, they have accumulated high levels active ingredients, resulting not only in medicinal value but also strong ability respond harsh environments. The TCP gene family plays an important role plant growth and development, signal transduction. However, these genes been systematically investigated species. In this study, we detected a...