A5042556775- CAR-T cell therapy research
- RNA Interference and Gene Delivery
- Nanowire Synthesis and Applications
- Viral Infectious Diseases and Gene Expression in Insects
- Monoclonal and Polyclonal Antibodies Research
- CRISPR and Genetic Engineering
- Force Microscopy Techniques and Applications
- Innovative Teaching Methods
- Glycosylation and Glycoproteins Research
- Immune Cell Function and Interaction
- SARS-CoV-2 and COVID-19 Research
- Immunotherapy and Immune Responses
- Lipid Membrane Structure and Behavior
- Biomedical and Engineering Education
- Molecular Junctions and Nanostructures
- Advanced biosensing and bioanalysis techniques
- Virus-based gene therapy research
University of Pennsylvania
2022-2024
Cornell University
2019-2020
Abstract With six therapies approved by the Food and Drug Association, chimeric antigen receptor (CAR) T cells have reshaped cancer immunotherapy. However, these rely on ex vivo viral transduction to induce permanent CAR expression in cells, which contributes high production costs long‐term side effects. Thus, this work aims develop an cell engineering platform streamline while using mRNA transient, tunable expression. Specifically, ionizable lipid nanoparticle (LNP) is utilized as platforms...
Abstract Chimeric antigen receptor (CAR) T cell therapy has achieved remarkable clinical success in the treatment of hematological malignancies. However, producing these bespoke cancer‐killing cells is a complicated ex vivo process involving leukapheresis, artificial activation, and CAR construct introduction. The activation step requires engagement CD3/TCR CD28 vital for transfection differentiation. Though antigen‐presenting (APCs) facilitate vivo, relies on antibodies against CD3...
Immune modulation through the intracellular delivery of nucleoside-modified mRNA to immune cells is an attractive approach for in vivo immunoengineering, with applications infectious disease, cancer immunotherapy, and beyond. Lipid nanoparticles (LNPs) have come fore as a promising nucleic acid platform, but LNP design criteria remain poorly defined, making rate-limiting step discovery screening process. In this study, we employed high-throughput based on molecular barcoding investigate...
Abstract A graduate course on vaccine development challenged students to propose concepts improve upon current paradigms in the context of a “Shark Tank‐style” format where were asked develop an abbreviated business plan and make pitch “Sharks” (experienced academic industrial researchers developers) they could request funding, research collaborations or regulatory guidance. Students graded based components their ability convince feasibility innovation potential project proposals. This...
Precise pH measurements in the immediate environment of receptors is essential for elucidating mechanisms through which local changes associated with diseased phenotypes manifest into aberrant receptor function. However, current sensors lack ability to localize and target specific molecules required make these measurements. Herein we present Litmus-body, our recombinant protein-based sensor, fusion an anti-IgG nanobody capable piggybacking on IgG antibodies molecular targeting proteins cell...
ABSTRACT Precise pH measurements in the immediate environment of receptors is essential for elucidating mechanisms through which local changes associated with diseased phenotypes manifest into aberrant receptor function. However, current sensors lack molecular specificity required to make these measurements. Herein we present Litmus-body, our recombinant protein-based sensor, fusion an anti-mouse IgG nanobody capable targeting specific proteins on cell surface. By normalizing a pH-dependent...