A5010526490- RNA Interference and Gene Delivery
- Nanoplatforms for cancer theranostics
- Advanced biosensing and bioanalysis techniques
- Nanoparticle-Based Drug Delivery
- CAR-T cell therapy research
- MicroRNA in disease regulation
- Extracellular vesicles in disease
- Immunotherapy and Immune Responses
- Peptidase Inhibition and Analysis
- SARS-CoV-2 and COVID-19 Research
- Virus-based gene therapy research
- Carbon and Quantum Dots Applications
- Nanowire Synthesis and Applications
- Monoclonal and Polyclonal Antibodies Research
- Nanocluster Synthesis and Applications
- Pancreatic and Hepatic Oncology Research
- Supramolecular Self-Assembly in Materials
- Bacteriophages and microbial interactions
- Lipid Membrane Structure and Behavior
- Cancer, Hypoxia, and Metabolism
- Immune Cell Function and Interaction
- Epigenetics and DNA Methylation
- Cancer, Stress, Anesthesia, and Immune Response
- RNA and protein synthesis mechanisms
- Immune cells in cancer
University of Pennsylvania
2020-2025
Center for Excellence in Molecular Cell Science
2024-2025
Nanjing University of Chinese Medicine
2021-2025
University of Chinese Academy of Sciences
2017-2024
National Center for Nanoscience and Technology
2017-2021
University of Alberta
2021
Tsinghua University
2017-2019
Cambridge University Press
2011
New York University Press
2011
Photodynamic therapy (PDT) is a promising anticancer treatment and clinically approved for different types of tumors. However, current PDT suffers several obstacles, including its neutralization by excess glutathione (GSH) in the tumor tissue strongly proangiogenic response. In this work, biomimic, multifunctional nanoparticle-based agent, combining tumor-targeted photosensitizer with GSH scavenging antiangiogenesis therapy, developed. A porphyrinic Zr-metal-organic framework nanoparticle...
Recent years have witnessed incredible growth in RNA therapeutics, which has benefited significantly from decades of research on lipid nanoparticles, specifically its key component—the ionizable lipid. This comment discusses the major types, and provides perspectives for future development. therapeutics
An effective tumor vaccine vector that can rapidly display neoantigens is urgently needed. Outer membrane vesicles (OMVs) strongly activate the innate immune system and are qualified as immunoadjuvants. Here, we describe a versatile OMV-based platform to elicit specific anti-tumor response via specifically presenting antigens onto OMV surface. We first on OMVs surface by fusing with ClyA protein, then simplify antigen process employing Plug-and-Display comprising tag/catcher protein pairs....
Abstract Cysteine‐based chiral optically active carbon dots (CDs) and their effects on cellular energy metabolism, which is vital for essential functions, have been barely reported. A green effective synthesis strategy N‐S‐doped CDs (fluorescence quantum yield ca. 41.26 %) based hydrothermal treatment of l ‐ or d ‐cysteine at as low 60 °C has developed. This suggested that cysteine was instable in aqueous solutions acts a warning high‐temperature nanomaterials using stabilizer. Human bladder...
Pancreatic ductal adenocarcinoma is characterised by a dense desmoplastic stroma composed of stromal cells and extracellular matrix (ECM). This barrier severely impairs drug delivery penetration. Activated pancreatic stellate (PSCs) play key role in establishing this unique pathological obstacle, but also offer potential target for anti-tumour therapy. Here, we construct tumour microenvironment-responsive nanosystem, based on PEGylated polyethylenimine-coated gold nanoparticles, utilise it...
Excess iron deposition in the brain often causes oxidative stress-related damage and necrosis of dopaminergic neurons substantia nigra has been reported to be one major vulnerability factors Parkinson's disease (PD). Iron chelation therapy using deferoxamine (DFO) may inhibit this nigrostriatal degeneration prevent progress PD. However, DFO shows very short half-life vivo hardly penetrates blood barrier (BBB). Hence, it is great interest develop formulations for safe efficient intracerebral...
The development of lipid nanoparticle (LNP) formulations for targeting the bone microenvironment holds significant potential nucleic acid therapeutic applications including regeneration, cancer, and hematopoietic stem cell therapies. However, delivery to remains a challenge due several biological barriers, such as low blood flow in bone, blood–bone marrow affinity between drugs minerals, which leads unfavorable dosages microenvironment. Here, we construct series bisphosphonate (BP)...
Abstract Lipid nanoparticle-mediated RNA delivery holds great potential to treat various liver diseases. However, targeted of therapeutics activated liver-resident fibroblasts for fibrosis treatment remains challenging. Here, we develop a combinatorial library anisamide ligand-tethered lipidoids (AA-lipidoids) using one-pot, two-step modular synthetic method and adopt two-round screening strategy identify AA-lipidoids with both high potency selectivity deliver payloads fibroblasts. The lead...
Abstract With six therapies approved by the Food and Drug Association, chimeric antigen receptor (CAR) T cells have reshaped cancer immunotherapy. However, these rely on ex vivo viral transduction to induce permanent CAR expression in cells, which contributes high production costs long‐term side effects. Thus, this work aims develop an cell engineering platform streamline while using mRNA transient, tunable expression. Specifically, ionizable lipid nanoparticle (LNP) is utilized as platforms...
Lipid nanoparticles (LNPs) are a potent delivery technology that have made it possible for the recent clinical breakthroughs in mRNA therapeutics and vaccines. A key challenge to broader implementation of vaccines is development produce precisely defined LNP formulations, with throughput can scale from discovery commercial manufacturing meet stringent standards pharmaceutical industry. To address these challenges, we developed microfluidic chip incorporates 1×, 10×, or 256× LNP-generating...
Abstract Lipid nanoparticles for delivering mRNA therapeutics hold immense promise the treatment of a wide range lung-associated diseases. However, lack effective methodologies capable identifying pulmonary delivery profile chemically distinct lipid libraries poses significant obstacle to advancement therapeutics. Here we report implementation barcoded high-throughput screening system as means identify lung-targeting efficacy cationic, degradable lipid-like materials. We combinatorially...
Abstract Chimeric antigen receptor (CAR) T cell therapy has achieved remarkable clinical success in the treatment of hematological malignancies. However, producing these bespoke cancer‐killing cells is a complicated ex vivo process involving leukapheresis, artificial activation, and CAR construct introduction. The activation step requires engagement CD3/TCR CD28 vital for transfection differentiation. Though antigen‐presenting (APCs) facilitate vivo, relies on antibodies against CD3...
Disruption of pulmonary vascular homeostasis is a central feature viral pneumonia, wherein endothelial cell (EC) death and subsequent angiogenic responses are critical determinants the outcome severe lung injury. A more granular understanding fundamental mechanisms driving reconstitution endothelium necessary to facilitate therapeutic repair. Here, we demonstrated that TGF-β signaling through TGF-βR2 (transforming growth factor–β receptor 2) activated in ECs upon influenza infection, mice...
Abstract The ionizable lipidoid is a key component of lipid nanoparticles (LNPs). Degradable lipidoids containing extended alkyl branches have received tremendous attention, yet their optimization and investigation are underappreciated. Here, we devise an in situ construction method for the combinatorial synthesis degradable branched (DB) lipidoids. We find that appending branch tails to inefficacious via linkers boosts mRNA delivery efficiency up three orders magnitude. Combinatorial...
Pancreatic cancer, one of the leading causes cancer-related mortality, is characterized by desmoplasia and hypovascular cancerous tissue, with a 5 year survival rate <8%. To overcome severe resistance pancreatic cancer to conventional therapies, we synthesized gold nanoshell-coated rod-like mesoporous silica (GNRS) nanoparticles which integrated cascade tumor targeting (mediated photothermal effect molecular receptor binding) treatment-enhanced gemcitabine chemotherapy, under mild...
Prostate cancer is one of the most common malignant tumors in men, and inhibiting metastasis a key event but still major challenge prostate treatment. Cancer-associated fibroblasts (CAFs) play an important role tumor by shaping microenvironment. Herein, we constructed CAF-targeting siRNA delivery system loading fibroblast activation protein-α (FAP-α) antibody onto cell-penetrating peptide (CPP)-based nanoparticles, which specifically downregulated C–X–C motif chemokine ligand 12 (CXCL12)...
Platinum (Pt)-based chemotherapy is a broadly used therapeutic regimen against various cancers. However, the insufficient cellular uptake, deactivation by thiol-containing species and nonspecific distribution of cisplatin (CDDP) result in its low chemosensitivity as well systemic side effects, which can largely constrain employment CDDP clinical treatment. To circumvent these problems, this study, polymeric nanoparticles were utilized to codeliver water-soluble derivative, poly(γ,l-glutamic...
Abstract Tumor complexity makes the development of highly sensitive tumor imaging probes an arduous task. Here, we construct a peptide‐based near‐infrared probe that is responsive to fibroblast activation protein‐α (FAP‐α), and specifically forms nanofibers on surface cancer‐associated fibroblasts (CAFs) in situ. The assembly/aggregation‐induced retention (AIR) effect results enhanced accumulation around tumor, resulting 5.5‐fold signal enhancement 48 h after administration compared control...
The limited efficacy of single-agent immune checkpoint inhibitors in treating tumors has prompted investigations on their combination partners. Here, a tumor-homing indoleamine 2,3-dioxygenase (IDO) nanoinhibitor is reported to selectively inhibit immunosuppressive IDO pathway the tumor microenvironment. It self-assembled from modularly designed peptide-drug conjugate containing hydrophilic targeting motif (arginyl-glycyl-aspartic acid; RGD), two protonatable histidines, and an ester...
Abstract Fenton reaction‐mediated oncotherapy is an emerging strategy which uses iron ions to catalytically convert endogenous hydrogen peroxide into hydroxyl radicals, the most reactive oxygen species found in biology, for efficient cancer therapy. However, reaction efficiency tumor tissue typically limited due restrictive conditions. One overcome this obstacle increase temperature specifically at site. Herein, a tumor‐targeting sponge (TTIS) nanocomposite based on graphdiyne oxide, has...
Although postsurgical chemotherapy is frequently used for the treatment of breast cancer, tumor recurrence still a frequent event. Enhancing efficacy via localized drug delivery may help to prevent cancer recurrence. To achieve this goal, we designed hydrogel nanocarrier that could be injected at site by coassembly tailor-made hexapeptide and doxorubicin. Evidently, on basis our findings, sustained release from led reduction in recurrence, including suppression primary regrowth distant...