A5006087990- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- CAR-T cell therapy research
- Multiple Myeloma Research and Treatments
- Nanoparticle-Based Drug Delivery
- Immunotherapy and Immune Responses
- Nanowire Synthesis and Applications
- Cell Adhesion Molecules Research
- Graphene and Nanomaterials Applications
- Signaling Pathways in Disease
- MicroRNA in disease regulation
- Animal Virus Infections Studies
- Pregnancy and preeclampsia studies
- Reproductive System and Pregnancy
- Pregnancy and Medication Impact
- Viral Infectious Diseases and Gene Expression in Insects
- Virus-based gene therapy research
University of Pennsylvania
2020-2025
Abstract Lipid nanoparticles for delivering mRNA therapeutics hold immense promise the treatment of a wide range lung-associated diseases. However, lack effective methodologies capable identifying pulmonary delivery profile chemically distinct lipid libraries poses significant obstacle to advancement therapeutics. Here we report implementation barcoded high-throughput screening system as means identify lung-targeting efficacy cationic, degradable lipid-like materials. We combinatorially...
Abstract Chimeric antigen receptor (CAR) T cell therapy has achieved remarkable clinical success in the treatment of hematological malignancies. However, producing these bespoke cancer‐killing cells is a complicated ex vivo process involving leukapheresis, artificial activation, and CAR construct introduction. The activation step requires engagement CD3/TCR CD28 vital for transfection differentiation. Though antigen‐presenting (APCs) facilitate vivo, relies on antibodies against CD3...
Multiple myeloma (MM), a hematologic malignancy that preferentially colonizes the bone marrow, remains incurable with survival rate of 3 to 6 mo for those advanced disease despite great efforts develop effective therapies. Thus, there is an urgent clinical need innovative and more MM therapeutics. Insights suggest endothelial cells within marrow microenvironment play critical role. Specifically, cyclophilin A (CyPA), homing factor secreted by (BMECs), homing, progression, survival,...
Lipid nanoparticles (LNPs) have emerged as pivotal vehicles for messenger RNA (mRNA) delivery to hepatocytes upon systemic administration and antigen-presenting cells following intramuscular injection. However, achieving mRNA non-hepatocytes remains challenging without the incorporation of targeting ligands such antibodies, peptides, or small molecules. Inspired by comb-like polymeric architecture, here we utilized a multiarm-assisted design construct library 270 dendron-like degradable...
In the past 10 years, CRISPR-Cas9 has revolutionized gene-editing field due to its modularity, simplicity, and efficacy. It been applied for creation of in vivo models, further understand human biology, toward curing genetic diseases. However, there remain significant delivery barriers application clinic, especially extrahepatic applications. this work, high-throughput molecular barcoding techniques were used alongside traditional screening methodologies simultaneously evaluate LNP...