A5023105714- RNA Interference and Gene Delivery
- Polymer composites and self-healing
- Advanced Materials and Mechanics
- Advanced biosensing and bioanalysis techniques
- Advanced Sensor and Energy Harvesting Materials
- Nanoparticle-Based Drug Delivery
- Supramolecular Self-Assembly in Materials
- Polymer Surface Interaction Studies
- Advanced Polymer Synthesis and Characterization
- Virus-based gene therapy research
- Surface Modification and Superhydrophobicity
- CAR-T cell therapy research
- Synthetic Organic Chemistry Methods
- Photochromic and Fluorescence Chemistry
- Photonic Crystals and Applications
- Bone health and treatments
- Synthesis of heterocyclic compounds
- MicroRNA in disease regulation
- CRISPR and Genetic Engineering
- Enzyme Catalysis and Immobilization
- Remote Sensing in Agriculture
- Smart Agriculture and AI
- Luminescence and Fluorescent Materials
- Immunotherapy and Immune Responses
- Fuel Cells and Related Materials
Nanjing University
2025
University of Pennsylvania
2022-2025
Nanjing Medical University
2025
California University of Pennsylvania
2025
University of Electronic Science and Technology of China
1990-2024
Huzhou University
2023-2024
Ministry of Industry and Information Technology
2024
Harbin Institute of Technology
2024
Beijing Institute of Graphic Communication
2020-2023
Leibniz-Institute for New Materials
2018-2022
The development of lipid nanoparticle (LNP) formulations for targeting the bone microenvironment holds significant potential nucleic acid therapeutic applications including regeneration, cancer, and hematopoietic stem cell therapies. However, delivery to remains a challenge due several biological barriers, such as low blood flow in bone, blood–bone marrow affinity between drugs minerals, which leads unfavorable dosages microenvironment. Here, we construct series bisphosphonate (BP)...
Abstract Lipid nanoparticle-mediated RNA delivery holds great potential to treat various liver diseases. However, targeted of therapeutics activated liver-resident fibroblasts for fibrosis treatment remains challenging. Here, we develop a combinatorial library anisamide ligand-tethered lipidoids (AA-lipidoids) using one-pot, two-step modular synthetic method and adopt two-round screening strategy identify AA-lipidoids with both high potency selectivity deliver payloads fibroblasts. The lead...
Abstract With six therapies approved by the Food and Drug Association, chimeric antigen receptor (CAR) T cells have reshaped cancer immunotherapy. However, these rely on ex vivo viral transduction to induce permanent CAR expression in cells, which contributes high production costs long‐term side effects. Thus, this work aims develop an cell engineering platform streamline while using mRNA transient, tunable expression. Specifically, ionizable lipid nanoparticle (LNP) is utilized as platforms...
Abstract Lipid nanoparticles for delivering mRNA therapeutics hold immense promise the treatment of a wide range lung-associated diseases. However, lack effective methodologies capable identifying pulmonary delivery profile chemically distinct lipid libraries poses significant obstacle to advancement therapeutics. Here we report implementation barcoded high-throughput screening system as means identify lung-targeting efficacy cationic, degradable lipid-like materials. We combinatorially...
Disruption of pulmonary vascular homeostasis is a central feature viral pneumonia, wherein endothelial cell (EC) death and subsequent angiogenic responses are critical determinants the outcome severe lung injury. A more granular understanding fundamental mechanisms driving reconstitution endothelium necessary to facilitate therapeutic repair. Here, we demonstrated that TGF-β signaling through TGF-βR2 (transforming growth factor–β receptor 2) activated in ECs upon influenza infection, mice...
Abstract The ionizable lipidoid is a key component of lipid nanoparticles (LNPs). Degradable lipidoids containing extended alkyl branches have received tremendous attention, yet their optimization and investigation are underappreciated. Here, we devise an in situ construction method for the combinatorial synthesis degradable branched (DB) lipidoids. We find that appending branch tails to inefficacious via linkers boosts mRNA delivery efficiency up three orders magnitude. Combinatorial...
Lipid nanoparticles (LNPs) play a crucial role in delivering messenger RNA (mRNA) therapeutics for clinical applications, including COVID-19 mRNA vaccines. While can be chemically modified to become immune-silent and increase protein expression, LNPs still trigger innate immune responses cause inflammation-related adverse effects. Inflammation turn suppress translation reduce the therapeutic effect. Dexamethasone (Dex) is widely used anti-inflammatory corticosteroid medication that...
Abstract Many organisms produce stunning optical displays based on structural color instead of pigmentation. This or photonic is achieved through the interaction light with intricate micro-/nano-structures, which are “grown” from strong, sustainable biological materials such as chitin, keratin, and cellulose. In contrast, current synthetic colored usually brittle, inert, produced via energy-intensive processes, posing significant challenges to their practical uses. Inspired by brilliantly...
Evasion of apoptosis is a hallmark cancer, attributed in part to overexpression the anti-apoptotic protein B-cell lymphoma 2 (Bcl-2). In variety cancer types, including lymphoma, Bcl-2 overexpressed. Therapeutic targeting has demonstrated efficacy clinic and subject extensive clinical testing combination with chemotherapy. Therefore, development co-delivery systems for agents, such as small interfering RNA (siRNA), chemotherapeutics, doxorubicin (DOX), holds promise enabling therapies. Lipid...
Multiple myeloma (MM), a hematologic malignancy that preferentially colonizes the bone marrow, remains incurable with survival rate of 3 to 6 mo for those advanced disease despite great efforts develop effective therapies. Thus, there is an urgent clinical need innovative and more MM therapeutics. Insights suggest endothelial cells within marrow microenvironment play critical role. Specifically, cyclophilin A (CyPA), homing factor secreted by (BMECs), homing, progression, survival,...
Abstract Inflammation induced by lung infection is a double-edged sword, moderating both anti-viral and immune pathogenesis effects; the mechanism of latter not fully understood. Previous studies suggest vasculature involved in tissue injury. Here, we report that expression Sparcl1, secreted matricellular protein, upregulated pulmonary capillary endothelial cells (EC) during influenza-induced Endothelial overexpression SPARCL1 promotes detrimental inflammation, with inducing ‘M1-like’...
Monogenic blood diseases are among the most common genetic disorders worldwide. These result in significant pediatric and adult morbidity, some can death prior to birth. Novel ex vivo hematopoietic stem cell (HSC) gene editing therapies hold tremendous promise alter therapeutic landscape but not without potential limitations. In offer a potentially safer more accessible treatment for these hindered by lack of delivery vectors targeting HSCs, which reside difficult-to-access bone marrow...
Abstract Natural organic structures form via a growth mode in which nutrients are absorbed, transported, and integrated. In contrast, synthetic architectures constructed through fundamentally different methods, such as assembling, molding, cutting, printing. Here, we report photoinduced strategy for regulating the localized of microstructures from surface swollen dynamic substrate, by coupling photolysis, photopolymerization, transesterification together. Photolysis is used to generate...
mRNA therapeutics are promising platforms for protein replacement therapies and gene editing technologies. When delivered via non-viral delivery systems, such as lipid nanoparticles (LNPs), easy to produce show low toxicity immunogenicity. However, LNPs limited efficiency tissue specificity in certain applications. To overcome this, we designed RGD peptide (Arg-Gly-Asp) based ionizable lipids, which can be formulated into integrin binding on cells targeted delivery. RGD-LNPs were using...
Macrophages are integral components of the innate immune system, playing a dual role in host defense during infection and pathophysiological states. contribute to responses aid combatting various infections, yet their production abundant proinflammatory cytokines can lead uncontrolled inflammation worsened tissue damage. Therefore, reducing macrophage-derived cytokine release represents promising approach for treating acute chronic inflammatory disorders. However, limited macrophage-specific...
Lipid nanoparticles (LNPs) have emerged as pivotal vehicles for messenger RNA (mRNA) delivery to hepatocytes upon systemic administration and antigen-presenting cells following intramuscular injection. However, achieving mRNA non-hepatocytes remains challenging without the incorporation of targeting ligands such antibodies, peptides, or small molecules. Inspired by comb-like polymeric architecture, here we utilized a multiarm-assisted design construct library 270 dendron-like degradable...