A5029069852- Advanced Breast Cancer Therapies
- Estrogen and related hormone effects
- HER2/EGFR in Cancer Research
- Breast Cancer Treatment Studies
- Cancer Immunotherapy and Biomarkers
- Immunotherapy and Immune Responses
- Cancer Cells and Metastasis
- CAR-T cell therapy research
- Cancer Treatment and Pharmacology
- Retinoids in leukemia and cellular processes
- Cancer Genomics and Diagnostics
- Monoclonal and Polyclonal Antibodies Research
- Ferroptosis and cancer prognosis
- Electrolyte and hormonal disorders
- Chronic Lymphocytic Leukemia Research
- Bioinformatics and Genomic Networks
- Gene expression and cancer classification
- Myasthenia Gravis and Thymoma
- Radiopharmaceutical Chemistry and Applications
- Lung Cancer Research Studies
- Cancer-related Molecular Pathways
- Ion Transport and Channel Regulation
- Immunodeficiency and Autoimmune Disorders
- RNA regulation and disease
- Melanoma and MAPK Pathways
Istituto di Ricovero e Cura a Carattere Scientifico San Raffaele
2022-2025
Istituti di Ricovero e Cura a Carattere Scientifico
2019-2025
Vita-Salute San Raffaele University
2022-2025
IRCCS Ospedale San Raffaele
2020-2024
San Raffaele University of Rome
2022
Fondazione IRCCS Istituto Neurologico Carlo Besta
2014-2019
Institute of Neuroimmunology of the Slovak Academy of Sciences
2016
University of Milano-Bicocca
2016
Antibody-drug conjugates (ADCs) are a rapidly expanding class of compounds in oncology. Our goal was to assess the expression ADC targets and potential downstream determining factors activity across pan-cancer normal tissues.
We assessed the 27-gene RT-qPCR-based DetermaIO assay and same score calculated from RNA sequencing (RNA-seq) data as predictors of sensitivity to immune checkpoint therapy in neoTRIPaPDL1 randomized trial that compared neoadjuvant carboplatin/nab-paclitaxel chemotherapy (CT) plus atezolizumab with CT alone stage II/III triple-negative breast cancer. also predictive function immuno-oncology (IO) expression patients treated pembrolizumab paclitaxel (N = 29) or 56) I-SPY2 trial.
Abstract Presurgical monitoring with intracerebral electrodes in patients drug‐resistant focal epilepsy represents a standard invasive procedure to localize the sites of seizures origin, defined as epileptogenic zone (EZ). During presurgical evaluation, single‐pulse electrical stimulation (SPES) is performed define boundaries eloquent areas and evoke seizure‐associated symptoms. Extensive intracranial exploration generate large dataset on brain connectivity that can be used improve EZ...
Autophagy has a large range of physiological functions and its dysregulation contributes to several human disorders, including autoinflammatory/autoimmune diseases such as inflammatory myopathies (IIMs). In order better understand the pathogenetic mechanisms these muscular we sought define role autophagic processes their relation with innate immune system in three main subtypes IIM, specifically sporadic inclusion body myositis (sIBM), polymyositis (PM), dermatomyositis (DM) juvenile (JDM)....
Abstract Background Despite huge efforts to identify biomarkers associated with long-term clinical outcomes in patients early-stage HER2-positive breast cancer (HER2+ BC) treated (neo)adjuvant anti-HER2 therapy, no reliable predictors have been identified so far. Fatty acid uptake, a process mediated by the transmembrane transporter CD36, has recently emerged as potential determinant of resistance treatments preclinical HER2+ BC models. Methods Here, we investigated association between...
Abstract Purpose: The interplay between estrogen receptor (ER) and erbB tyrosine-kinase receptors (RTK) impacts growth progression of ER-positive (ER+)/HER2-positive (HER2+) breast cancer generates mitogenic signals converging onto the Cyclin-D1/CDK4/6 complex. We probed this cross-talk combining endocrine-therapy (fulvestrant), dual HER2-blockade (trastuzumab pertuzumab), CDK4/6-inhibition (palbociclib; PFHPert). Experimental Design: Cytotoxic drug effects, interactions, pharmacodynamics...
Gain-of-function mutations in SCN9A gene that encodes the voltage-gated sodium channel NaV1.7 have been associated with a wide spectrum of painful syndromes humans including inherited erythromelalgia, paroxysmal extreme pain disorder and small fibre neuropathy. These change biophysical properties channels leading to hyperexcitability dorsal root ganglion nociceptors symptoms. There is need for better understanding how gain-of-function alter atomic structure Nav1.7. We used homology modeling...
The thymus plays a key role in myasthenia gravis (MG), B cell-mediated autoimmune disorder affecting neuromuscular junction. Most MG patients have thymic abnormalities, including hyperplasia and thymoma, neoplasm of epithelial cells. Epstein-Barr virus (EBV) is associated with diseases tumors. Recently, we showed EBV persistence reactivation hyperplastic thymuses, suggesting that might contribute to intra-thymic cell dysregulation patients. Here, investigated involvement thymoma-associated...
To characterize expression of neuregulin-1 (NRG1), an HER3 ligand, in HER2-positive breast cancer and its relation with the efficacy trastuzumab or without pertuzumab.Characterization NRG1 tumor cell lines, specimens, cancer-associated fibroblasts (CAFs). Patient-derived CAFs were used to investigate impact on activity pertuzumab cells. The relationship between pathologic response anti-HER2-based neoadjuvant therapy was assessed a retrospective patient cohort NeoSphere trial.NRG1 expressed...
The recently discovered meningeal lymphatic vessels (mLVs) have been proposed to be the missing link between immune and central nervous system. role of mLVs in modulating neuro-immune response following a traumatic brain injury (TBI), however, has not analyzed. Parenchymal T lymphocyte infiltration previously reported as part secondary events after TBI, suggestive an adaptive response. phenotype these cells remained mostly uncharacterized. In this study, we identified subpopulations...
Abstract Background Luminal breast cancers with high proliferation (MKS hi ) and low ER-related signalling (ERS lo have a poor prognosis. We investigated treatment responses molecular features of MKS /ERS tumours to inform potential therapies. Methods Gene expression data from patients who received neoadjuvant chemotherapy (NAC) without (MDACC, N = 199) or pembrolizumab (I-SPY2, 40), endocrine therapy (NET) (POETIC, 172) palbociclib (NeoPalAna, 32) were analyzed assess response by...
Nonconvulsive status epilepticus (ncSE) is a severe condition that may result in neurologic sequelae and epilepsy resistant to pharmacologic treatment. We analyze here seizure electroencephalography (EEG) patterns their correlation the development of chronic epileptic guinea pig model focal ncSE induced by intrahippocampal injection kainic acid (KA).Electrobehavioral during unilateral 1 μg KA CA1 region hippocampus were characterized continuous video-EEG monitoring 13 pigs bilaterally...
<div>AbstractPurpose:<p>We assessed the 27-gene RT-qPCR–based DetermaIO assay and same score calculated from RNA sequencing (RNA-seq) data as predictors of sensitivity to immune checkpoint therapy in neoTRIPaPDL1 randomized trial that compared neoadjuvant carboplatin/nab-paclitaxel chemotherapy (CT) plus atezolizumab with CT alone stage II/III triple-negative breast cancer. We also predictive function immuno-oncology (IO) expression patients treated pembrolizumab paclitaxel...
Abstract Background The identification of biomarkers for optimization immune checkpoint inhibitors (ICI) treatment is an unmet clinical need. In the Phase III randomized trial, NeoTRIPaPDL1, a post-hoc analysis whole transcriptome RNA-Seq data, previously showed that 27-gene IO score potential predictive biomarker increased pathological complete response with addition atezolizumab to carboplatin/nab-paclitaxel (Bianchini G ESMO 2021). However, laboratory implementation gene-expression...
Oculopharyngeal muscular dystrophy (OPMD) is a late-onset muscle disease caused by an abnormal (GCN) triplet expansion within the polyadenylate-binding protein nuclear 1 gene and consequent mRNA processing impairment myogenic defects. Because reduced cell proliferation potential regeneration failure of aging have been shown to be governed lethal-7 (let-7) microRNA-mediated mechanisms, in present study, we evaluated role let-7 pathogenesis OPMD. By multidisciplinary approach, including conf...
Abstract Background A post-hoc of NeoTRIP trial showed that 27-gene IO score assessed on baseline samples is predictive increased pathological complete response (pCR) with the addition atezolizumab to carboplatin/nab-paclitaxel, whereas LAR subtype has lowest rate pCR and without (Bianchini G ESMO 2021). We evaluated TNBC subtypes biopsies collected during treatment, biomarker dynamics, studied association pCR. Methods In NeoTRIP, patients randomly received 8 cycles nab-paclitaxel/carbo...
We investigated the association of single nucleotide polymorphisms (SNPs) in drug-metabolizing enzymes and transporters (DMETs) with response to azathioprine (AZA) patients affected by myasthenia gravis (MG) determine possible genotype-phenotype correlations.Genomic DNA from 180 AZA-treated MG was screened through Affymetrix DMET platform, which characterizes 1931 SNPs 225 genes. The significant SNPs, identified be involved AZA response, were subsequently validated allelic discrimination...
As most erb‐b2 receptor tyrosine kinase 2 (HER2)‐positive breast cancer (BC) patients currently receive dual HER2‐targeting added to neoadjuvant chemotherapy, improved methods for identifying individual response, and assisting postsurgical salvage therapy, are needed. Herein, we evaluated the 41‐gene classifier trastuzumab advantage risk model (TRAR) as a predictive marker enrolled in NeoSphere trial. TRAR scores were computed from RNA of 350 pre‐ 166 post‐treatment tumor specimens. Overall,...
Abstract OBJECTIVES Primary thoracic synovial sarcoma (SS) is a rare, high-grade, malignancy. Involvement of vital organs frequent and may decrease the benefits surgical resection. We reviewed our practice at highly experienced thoracic-surgery centre to assess early- long-term outcomes after surgery. METHODS conducted retrospective, observational, single-centre study patients undergoing curative-intent surgery for primary SS between 1 January 2000 31 2021 as part multidisciplinary...
Abstract The prevalence of malignant cells in clinical specimens, or tumour purity, is affected by both intrinsic biological factors and extrinsic sampling bias. Molecular characterization large cohorts typically performed on bulk samples; data analysis interpretation can be biased purity variability. Transcription-based strategies to estimate have been proposed, but no breast cancer specific method available yet. We interrogated over 4400 expression profiles from 9 datasets develop validate...
Importance Clinical trials are the path to test and introduce new therapies in clinic. Trials that unable produce results represent inefficiency system may also undermine patient confidence drug development process. Objectives To survey immunotherapy clinical trial landscape of breast cancer between January 2004 April 2023 examine what fraction with primary completion date up November 30, 2022, failed report outcome, assessing proportion yielded positive describing features associated these...