A5019810659- Neuroscience and Neuropharmacology Research
- Ion channel regulation and function
- Memory and Neural Mechanisms
- Alzheimer's disease research and treatments
- Neurotransmitter Receptor Influence on Behavior
- Nicotinic Acetylcholine Receptors Study
- Cellular transport and secretion
- Ion Channels and Receptors
- Cardiac electrophysiology and arrhythmias
- Receptor Mechanisms and Signaling
- Pain Mechanisms and Treatments
- Lipid Membrane Structure and Behavior
- Tryptophan and brain disorders
- Phosphodiesterase function and regulation
- Neuropeptides and Animal Physiology
- Mitochondrial Function and Pathology
- Cholinesterase and Neurodegenerative Diseases
- Chemical Synthesis and Analysis
- Genetics and Neurodevelopmental Disorders
- Epilepsy research and treatment
- Neuroinflammation and Neurodegeneration Mechanisms
- Heart Rate Variability and Autonomic Control
University of Basel
2020-2024
Universidade de Vigo
2012-2022
University of Bristol
2016-2021
Fernandez Hospital
2019
CNS Research (United States)
2016
Boehringer Ingelheim (Germany)
2015
GABA
In the CA1 area of hippocampus N-methyl-d-aspartate receptors (NMDARs) mediate induction long-term depression (LTD), short-term potentiation (STP) and (LTP). All these forms synaptic plasticity can be readily studied in juvenile hippocampal slices but involvement particular NMDAR subunits different is currently unclear. Here, using NVP-AAM077, Ro 25-6981 UBP145 to target GluN2A-, 2B- 2D-containing NMDARs respectively, we show that GluN2B-containing (GluN2B) are involved LTD, STP LTP prepared...
Phosphodiesterase (PDE) inhibitors are currently considered promising therapeutic targets for treatment of cognitive impairment in diseases such as Schizophrenia and Alzheimer's disease. Inhibitors PDE2A PDE9A have emerged potential candidates shown to improve synaptic plasticity memory function animals. However, the functional relevance their putative different localization neuron is not understood. Thus, this study aims at elucidating presynaptic effects inhibition comparison PDE9A. For...
The synaptic connection from medial habenula (MHb) to interpeduncular nucleus (IPN) is critical for emotion-related behaviors and uniquely expresses R-type Ca 2+ channels (Cav2.3) auxiliary GABA B receptor (GBR) subunits, the K + -channel tetramerization domain-containing proteins (KCTDs). Activation of GBRs facilitates or inhibits transmitter release MHb terminals depending on IPN subnucleus, but role KCTDs unknown. We therefore examined localization function Cav2.3, GBRs, in this pathway...
Amyloid-β precursor protein (APP) regulates neuronal activity through the release of secreted APP (sAPP) acting at cell surface receptors. and sAPP were reported to bind extracellular sushi domain 1 (SD1) GABAB receptors (GBRs). A 17 amino acid peptide (APP17) derived from was sufficient for SD1 binding shown mimic inhibitory effect on neurotransmitter activity. The functional effects APP17 similar those GBR agonist baclofen blocked by a antagonist. These experiments led proposal that...
The persistently active protein kinase Mζ (PKMζ) has been found to be involved in the formation and maintenance of long-term memory. Most studies investigating PKMζ, however, have used either putatively unselective inhibitors or conventional knock-out animal models which compensatory mechanisms may occur. Here, we overexpressed an form PKMζ rat hippocampus, a structure highly memory formation, embedded several neural networks. We investigated PKMζ's influence on synaptic plasticity using...
The GluN2 subunits of N-methyl-d-aspartate receptors (NMDARs) are key drivers synaptic plasticity in the brain, where particular composition endows NMDAR complex with distinct pharmacological and physiological properties. Compared to GluN2A GluN2B subunits, far less is known about role GluN2D subunit plasticity. In this study, we have used a GluN2C/2D selective competitive antagonist, UBP145, combination global knockout (GluN2D KO) mouse line study contribution GluN2D-containing NMDARs...
Abstract GABA B receptors (GBRs), the G protein-coupled for GABA, regulate synaptic transmission throughout brain. A main function of GBRs is gating Cav2.2-type Ca 2+ channels. However, cellular compartment where stable GBR/Cav2.2 signaling complexes form remains unknown. In this study, we demonstrate that vesicular protein synaptotagmin-11 (Syt11) binds to both auxiliary GBR subunit KCTD16 and Cav2.2 Through these dual interactions, Syt11 recruits channels post-Golgi vesicles, thus...
Two-pore domain potassium channels (K2P) constitute major candidates for the regulation of background currents in mammalian cells. Channels TREK subfamily are also well positioned to play an important role sensory transduction due their sensitivity a large number physiological and physical stimuli (pH, mechanical, temperature). Following our previous report describing molecular expression different K2P vagal system, here we confirm that functionally expressed neurons from mouse nodose...
Tg2576 mice are widely used to study amyloid-dependent synaptic dysfunction related Alzheimer's disease. However, conflicting data have been reported for these with regard basal transmission as well the in vitro correlate of memory, long-term potentiation (LTP). Some studies show clear impairments, whereas others report no deficiency. The present uses hippocampal slices from 3-, 10-, and 15-month-old wild-type (WT) evaluate function each group, including experiments investigate transmission,...
Background and Purpose: Verapamil, a drug widely used in certain cardiac pathologies, exert its therapeutic effect mainly through the blockade of L-type calcium channels. However, we also know that both voltage-dependent potassium channels are blocked by verapamil. Because sympathetic neurons superior cervical ganglion (SCG) known to express good variety currents, finely tune activity, speculated verapamil on these SCG could explain part action this drug. To address question, decided study,...
Adherens junction-associated protein 1 (AJAP1) has been implicated in brain diseases; however, a pathogenic mechanism not identified. AJAP1 is widely expressed neurons and binds to γ-aminobutyric acid type B receptors (GBRs), which inhibit neurotransmitter release at most synapses the brain. Here, we show that selectively dendrites trans-synaptically recruits GBRs presynaptic sites of expressing AJAP1. We have identified several monoallelic
Summary The connection from medial habenula (MHb) to interpeduncular nucleus is critical for aversion- and addiction-related behaviors. This pathway unique in selective expression of R-type voltage-gated Ca 2+ channels (Cav2.3) its terminals, robust potentiation release via presynaptic GABA B receptors (GBRs). To understand the mechanism underlying this peculiar GBR effect, we examined localization function Cav2.3, GBR, auxiliary subunits, K + -channel tetramerization domain-containing...
The present protocol explains how to prepare a primary culture of isolated neurons from the mouse nodose ganglion.
Background and Purpose: Verapamil, a drug widely used in certain cardiac pathologies, exert its therapeutic effect mainly through the blockade of L-type calcium channels. However, we also know that both voltage-dependent potassium channels are blocked by verapamil. Because sympathetic neurons superior cervical ganglion (SCG) known to express good variety currents, finely tune activity, speculated verapamil on these SCG could explain part action this drug. To address question, decided study,...
Abstract Amyloid-β precursor protein (APP) regulates neuronal activity through the release of secreted APP (sAPP) acting at cell-surface receptors. and sAPP were reported to bind extracellular sushi domain 1 (SD1) GABA B receptors (GBRs). A 17 amino-acid peptide (APP17) derived from was sufficient for SD1 binding shown mimic inhibitory effect on neurotransmitter activity. The functional effects APP17 similar those GBR agonist baclofen blocked by a antagonist. These experiments led proposal...