A5068492568- Dermatologic Treatments and Research
- Bioactive Natural Diterpenoids Research
- Hair Growth and Disorders
- melanin and skin pigmentation
- Skin and Cellular Biology Research
- Wound Healing and Treatments
- Fungal Plant Pathogen Control
- Synthetic Organic Chemistry Methods
- Collagen: Extraction and Characterization
- Cancer and Skin Lesions
- Lipid Membrane Structure and Behavior
- Bioactive natural compounds
- Chemical Synthesis and Analysis
- Virus-based gene therapy research
- Mast cells and histamine
- Marine Sponges and Natural Products
- Laser Applications in Dentistry and Medicine
- Urticaria and Related Conditions
- Genetics, Aging, and Longevity in Model Organisms
- Biochemical Acid Research Studies
- Phytochemical compounds biological activities
- Periodontal Regeneration and Treatments
- Powdery Mildew Fungal Diseases
- Antioxidant Activity and Oxidative Stress
- Autoimmune Bullous Skin Diseases
Seventh People's Hospital of Shanghai
2019-2022
Shanghai University of Traditional Chinese Medicine
2020-2022
State Council of the People's Republic of China
2020
Hangzhou Hospital of Traditional Chinese Medicine
2014-2017
Oxyresveratrol could strongly inhibit tyrosinase activity and decrease melanin production<italic>in vitro</italic>and<italic>in vivo</italic>.
The aim of the present study was to detect expression key molecules, including transforming growth factor‑β1 (TGF-β1), phosphatidylinositol 3-kinase (PI3K), protein kinase B (Akt) TGF‑β1/mammalian target rapamycin (mTOR) pathway in pathological scar fibroblasts. Immunofluorescence, reverse transcription‑polymerase chain reaction (RT‑PCR) and western blot analysis were used molecules TGF‑β1, PI3K, Akt, mTOR fibroblasts normal skin tissue tissue. Immunofluorescence showed that PI3K Akt...
This study provides new insights into the effects of OEB treatment on antioxidant capacity and metabolism that suggest could be used as a good source natural antioxidants.
The aim of the study was to examine expression mammalian target rapamycin (mTOR)/70S6K signaling pathway in pathological scar fibroblasts and effects resveratrol (Res) intervention. mTOR 70S6K normal skin were detected by immunofluorescence following treatment with different concentrations Res. RT-PCR western blot analysis used detect mRNA protein, respectively. Immunofluorescence showed that significantly enhanced fibroblasts, mainly expressed nucleus, but not fibroblasts. after Res...
The aim of the present study was to investigate effect resveratrol on cell apoptosis, ability telomerase and human reverse transcriptase (hTERT) protein expression in A431 epidermoid carcinoma cells. cells were treated with different concentrations resveratrol, appearance then observed under a microscope. In addition, proliferation examined using an MTT assay, detected telomeric repeat amplification protocol-polymerase chain reaction-ELISA. Resveratrol significantly inhibited decreased hTERT...
Abstract Background: Alopecia areata is an autoimmune hair loss disease with infiltration of pro-inflammatory cells into follicles. The role Tgr5 in dermatitis has attracted considerable attention. present study aimed to investigate the effect development areata. Methods: utilized a comparison control group design four groups wild-type group, wild-type+INT777 Tgr5−/− and Tgr5−/−+INT777 group. mice were treated INT777 (30 mg/kg/day) or carrier solution (DMSO) intraperitoneally for 7 weeks,...
Two new lathyrane-type diterpenoids, jatropodagins A and B (1 2), five known analogues (3-7), were isolated from the stems of Jatropha podagrica. Their structures absolute configurations elucidated by spectroscopic data calculated ECD analyses. The cytotoxicities all diterpenoids (1-7) evaluated against two human osteosarcoma cell lines (Saos-2 MG-63). Compound 1 exhibited significant cytotoxic effects Saos-2 MG-63 with IC50 values 8.08 14.64 μM, respectively. for positive control 5-FU 19.01...
Objective Galla chinensis ointment can inhibit the proliferation of keloid fibroblasts and decrease formation. We investigated whether inhibits fibroblast through expression microRNA-21, phosphorylated (p)-phosphatidylinositol 3-kinase (p-PI3K), chromosome 10 neutropenic protein phosphatase (PTEN), kinase B (p-Akt), mammalian target rapamycin (p-mTOR). Methods A mouse model human keloid-derived were developed treated with chinensis. Immunohistochemistry, western blot, reverse...
To evaluate the effectiveness of Wubeizi (WBZ) ointment on keloid-derived fibroblasts. The primary cells fibroblasts were cultured and WBZ at different concentrations was examined by MTT colorimetric methods showed inhibitory effects proliferating (P < 0.01)in a time- dose-dependent manner. proportion in S stage significantly higher each group than control (P<0.01), G2 + M lower that group, which statistically significant 0.01).The increased with drug 0.05).The can inhibit proliferation...
Facial seborrheic dermatitis is a chronic skin condition that presents as erythematous scaly and has detrimental effect on the patient's quality of life. The purpose this article to present successful treatment two male patients with face dermatitis, one aged 50 other 56, who developed facial following erythema eruptions. We diagnosed it based patients' initial appearances location during episode. previous doctor symptoms related allergies or infections; therefore, they were treated...
Wubeizi (Rhus chinensis Mill.) ointment has been shown as an effective treatment for keloids. However, the protective mechanisms of are not fully understood. The mammalian target rapamycin (mTOR) demonstrated to be associated with keloid pathogenesis. In present study, we investigated if suppressed formation through modulation key molecules pathway including phosphatase and tensin homolog (PTEN), phosphatidylinositol 3-kinase (PI3K), protein kinase B (Akt).A mouse model human keloid-derived...
Previous studies from our lab have shown that the antimicrobial peptide F1 obtained milk fermentation by Lactobacillus paracasei FX-6 derived Tibetan kefir was different common peptides; specifically, simultaneously inhibited growth of Gram-negative and Gram-positive bacteria. Here, we present follow-on work demonstrating after acts on either Escherichia coli ATCC 25922 ( E. ) or Staphylococcus aureus 63589 S. ), their respective bacterial membranes were severely deformed. This deformation...
We evaluated the effect of Wubeizi (WBZ) ointment on keloids. Keloid-derived fibroblast primary cultures were used to evaluate different concentration WBZ expression type I and III procollagen in keloid using dot blot assay. Type II precollagen cDNA probes labeled with non-radioactive digoxin for blot. Cell divided into 4 groups: The large dose group received 1 g/ml WBZ, middle dose, small groups 0.5 0.25 respectively. control serum-free medium without WBZ. Our results showed that mRNA was...
BACKGROUND: We observed the effects of resveratrol on expression molecules involved in mTOR signaling pathway pathological scar fibroblasts, including PI3K, Akt and mTOR.METHODS: detected normal skin fibroblasts through immunofluorescence. After being treated with different concentrations resveratrol, mRNA protein were by RT-PCR Western Blot respectively.RESULTS: Results showed that significantly enhanced mainly expressed nucleus, no fibroblasts. from tests demonstrated after Res...
The ethyl acetate extraction (WCE) of Wikstroemia chamaedaphne Meisn on the viability melanoma cell lines B16 and A375 were evaluated.The WCE exhibited a potent cytotoxic against with IC50 values 156.2 192.8 µg/mL, respectively.Cell migration was assessed using wound healing assay.The caused cycle arrest in G0/G1 phase cells S cells, according to analysis.Flow cytometric analysis revealed that promoted apoptosis dosedependent manner.Chemical resulted isolation new diterpenoid, wikstchalin A...