A5042329097- Estrogen and related hormone effects
- Cytokine Signaling Pathways and Interactions
- RNA Research and Splicing
- Retinoids in leukemia and cellular processes
- Reproductive System and Pregnancy
- Phytoestrogen effects and research
- Animal Nutrition and Physiology
- Hypothalamic control of reproductive hormones
- Effects and risks of endocrine disrupting chemicals
- NF-κB Signaling Pathways
- RNA modifications and cancer
- Genomics, phytochemicals, and oxidative stress
- Computational Drug Discovery Methods
- Digestive system and related health
- Curcumin's Biomedical Applications
- Viral Infectious Diseases and Gene Expression in Insects
- Hormonal Regulation and Hypertension
- Cancer-related molecular mechanisms research
- Immune Cell Function and Interaction
- Immune cells in cancer
- Reproductive Biology and Fertility
- Nuclear Receptors and Signaling
- Fatty Acid Research and Health
- Genomics and Chromatin Dynamics
- Garlic and Onion Studies
National Institute of Environmental Health Sciences
2014-2024
National Institutes of Health
2009-2022
Triangle
2009-2019
Research Triangle Park Foundation
2015
Jichi Medical University
2000-2009
Japan Science and Technology Agency
2001-2005
The University of Tokyo
1996-2005
Community Medical Center
2004
Tokyo University of Agriculture
1994-1996
Shigei Medical Research Institute
1981
Endocrine-disrupting chemicals (EDCs) are widely found in the environment. Estrogen-like activity is attributed to EDCs, such as bisphenol A (BPA), AF (BPAF), and zearalenone (Zea), but mechanisms of action diversity effects poorly understood.We used vitro models evaluate mechanistic actions BPA, BPAF, Zea on estrogen receptor (ER) α ERβ.We three human cell lines (Ishikawa, HeLa, HepG2) representing types promoter ERα ERβ. Ishikawa/ERα stable cells were determine changes response element...
tetrakaidecasaccharides were separated within 60 min and the separation pattern was highly reproducible.The method applied for preparative of complex glycolipids with blood group activity.-Watanabe,K., Y. Arao.A new solvent system neutral glycosphingolipids.
Endocrine-disrupting chemicals (EDCs) influence the activity of estrogen receptors (ERs) and alter function endocrine system. However, diversity EDC effects mechanisms action are poorly understood.We examined agonistic EDCs through ERα ERβ. We also investigated on ER-mediated target genes.HepG2 HeLa cells were used to determine ERβ via luciferase reporter assay. Ishikawa stably expressing changes in endogenous ER gene expression by EDCs.Twelve categorized into three groups basis product...
Previous case reports associated prepubertal gynecomastia with lavender-containing fragrances, but there appear to be no of premature thelarche.To add a series about lavender-fragranced product use and breast growth in children measure endocrine-disrupting chemical activity essential oil components.Patients experiencing thelarche or continuous exposure products were evaluated the pediatric endocrinology departments two institutions. Mechanistic vitro experiments using eight components...
The estrogen receptor (ER) is a ligand-dependent transcription factor containing two transcriptional activation domains. AF-1 in the N terminus of protein and AF-2 activity dependent on helix 12 C-terminal ligand-binding domain. Two point mutations leucines 543 544 to alanines (L543A, L544A) minimized estrogen-dependent reversed antagonists ICI182780 (ICI) tamoxifen (TAM) into agonists similar manner that TAM activated WT ERα through activation. To evaluate physiological role for...
The three main mechanisms of ERα action are: 1) nuclear, genomic, direct DNA binding, 2) "tethered"-mediated, protein-protein interactions, and 3) non-nuclear, non-genomic, rapid responses. Reports suggest the D-domain or hinge region plays an important role in 1 2 above. Studies demonstrating functionality have resected full D-domain; therefore, site directed mutations were made to attribute precise sequence this domain. This study focuses on characterization properties novel ERα- mutants....
Diarylheptanoids isolated from Curcuma comosa Roxb. have been recently identified as phyto estrogens. However, the mechanism underlying their actions has not yet identified.We characterized estrogenic activity of three active naturally occurring diarylheptanoids both in vitro and vivo.We mechanisms action (3S)-1,7-diphenyl-(6E)-6-hepten-3-ol (D1), 1,7-diphenyl-(6E)-6-hepten-3-one (D2), (3R)-1,7-diphenyl-(4E,6E)-4,6-heptadien-3-ol (D3) by using a real-time polymerase chain reaction assay,...
The 9-cis-retinoic acid receptors (RXRs), belonging to the members of steroid/thyroid hormone receptor superfamily, act as auxiliary proteins, heterodimerizing with other nuclear such retinoic (RARs), vitamin D receptor, thyroid receptors, and peroxisome-proliferator activated thereby transactivating target genes in a ligand-dependent manner. We have previously reported that rat, (TH) positively negatively regulates hepatic mRNA levels RXR beta gamma, respectively. In present study, we tried...
Estrogen receptor alpha (ERα) is a ligand-dependent transcription factor containing two transcriptional activation function (AF) domains. AF-1 in the N terminus of protein, and AF-2 activity dependent on helix 12 C-terminal ligand-binding domain. We recently showed that point mutations converting leucines 543 544 to alanines (AF2ER) minimized estrogen-dependent activation. A characteristic feature AF2ER estrogen antagonists ICI182780 tamoxifen (TAM) act as agonists through intact AF-1, but...
Polycystic ovary syndrome (PCOS) is a hypothalamic-pituitary-gonadal (HPG) axis disorder. PCOS symptoms most likely result from disturbance in the complex feedback regulation system of HPG axis, which involves gonadotrophic hormones and ovarian steroid hormones. However, nature this interconnecting makes it difficult to dissect molecular mechanisms responsible for phenotypes. Global estrogen receptor α (ERα) knockout (KO) mice exhibit disruption resulting hormonal dysregulation female ERt*...
A ligand-dependent nuclear transcription factor, ERα has two transactivating functional domains (AF), AF-1 and AF-2. is localized in the N-terminal region, AF-2 distributed C-terminal ligand-binding domain (LBD) of protein. Helix 12 (H12) LBD a component AF-2, configuration H12 ligand-inducible to an active or inactive form. We demonstrated previously that mutant (AF2ER) possessing L543A,L544A mutations disrupts function reverses antagonists such as fulvestrant/ICI182780 (ICI)...
Human ovarian cancer BG-1 cells are a valuable in vitro model that has enabled several laboratories to study the estrogenic responses of cancers. We recently discovered there two different cell lines being used for experiments, denoted here as FR and NIEHS, which exhibit striking morphological differences. The objective this was methodically analyze these variants compare their characteristics. Short tandem repeat analysis revealed DNA profile unique, yet pattern NIEHS identical with MCF-7...
Estrogen stimulates breast development during puberty and mammary tumors in adulthood through estrogen receptor-α (ERα). These effects are proposed to occur via ERα+ luminal cells not the stem (MaSCs) that ERαneg. Since express cell antigen-1 (SCA-1), we sought determine if SCA-1 could define an subset of EpCAM+/CD24+/CD49fhi MaSCs. We show MaSC population has a distinct SCA-1+ is abundant pre-pubertal glands. The MaSCs have less markers vivo repopulating activity than their SCA-1neg...
Abstract Estrogen (E2) signaling through its nuclear receptor, E2 receptor α (ERα) increases insulinlike growth factor 1 (IGF1) in the rodent uterus, which then initiates further signals via IGF1 receptor. Directly administering results similar biological and transcriptional uterine responses. Our studies using global ERα-null mice demonstrated a loss of responses uterus to or treatment, while maintaining IGF1. To address this discrepancy need for ERα mediating vs responses, we assessed...