A5066973194- Renal Transplantation Outcomes and Treatments
- Cytomegalovirus and herpesvirus research
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Organ Transplantation Techniques and Outcomes
- Renal Diseases and Glomerulopathies
- Herpesvirus Infections and Treatments
- Prostate Cancer Treatment and Research
- Cancer Immunotherapy and Biomarkers
- Immunotherapy and Immune Responses
- Transplantation: Methods and Outcomes
- Cancer, Stress, Anesthesia, and Immune Response
- Prostate Cancer Diagnosis and Treatment
- HIV Research and Treatment
- CAR-T cell therapy research
- RNA Interference and Gene Delivery
- Complement system in diseases
- Estrogen and related hormone effects
- Viral Infections and Immunology Research
- Polyomavirus and related diseases
Stanford University
2014-2021
Beckman Research Institute
2019
City of Hope
2019
Institut d'Investigació Biomédica de Bellvitge
2011-2018
Stanford Medicine
2017
Universitat de Barcelona
2011-2015
Stanford Health Care
2014
GlaxoSmithKline (Canada)
2011
Bellvitge University Hospital
2011
University Health Network
2011
Cytomegalovirus (CMV) infection is still a major complication after kidney transplantation. Although cytotoxic CMV-specific T cells play crucial role controlling CMV survival and replication, current pretransplant risk assessment for only based on donor/recipient (IgG)-serostatus. Here, we evaluated the usefulness of monitoring pre- 6-month cell responses against two dominant antigens (IE-1 pp65) lysate, using an IFN-γ Elispot, predicting advent in cohorts 137 transplant recipients either...
Assessment of donor-specific alloreactive memory/effector T cell responses using an IFN-γ Elispot assay has been suggested to be a novel immune-monitoring tool for evaluating the cellular immune risk in renal transplantation. Here, we report cross-validation data performed within different European laboratories taking part EU RISET consortium. For this purpose, development standard operating procedure (SOP), comparisons lectures plates assessing intra- and interlaboratory variability...
Background. Cytomegalovirus (CMV) infection remains a major complication after kidney transplantation. Baseline CMV risk is typically determined by the serological presence of preformed CMV-specific immunoglobulin (Ig) G antibodies, even though T-cell responses to viral antigens are crucial when controlling replication. Some IgG-seronegative patients who receive an IgG-seropositive allograft do not develop despite receiving prophylaxis. We hypothesized that more precise evaluation...
Antibody-mediated rejection (ABMR) is defined by specific histopathological lesions and evidence of circulating donor-specific antibodies (DSA). Although DSA are not always detectable, monitoring donor-reactive memory B cells (mBC) could identify patients at risk developing ABMR. Peripheral mBC using a novel HLA cell ELISpot assay, serum DSA, numbers different subsets were assessed in 175 consecutive kidney transplants undergoing either for-cause or 6- 24-month surveillance biopsies for...
Presence of subclinical rejection (SCR) with IF/TA in protocol biopsies renal allografts has been shown to be an independent predictor factor graft loss. Also, intragraft Foxp3+ Treg cells patients SCR suggested differentiate harmful from potentially protective infiltrates. Nonetheless, whether presence Foxp3 and may protect a deleterious outcome not yet evaluated. This is case-control study which 37 the diagnosis 68 control no cellular infiltrates at 6-month matched for age time...
Currently, there are two Food and Drug Administration (FDA)-approved drugs for androgen deprivation therapy (ADT) of metastatic castration-resistant prostate cancer (mCRPC) patients: abiraterone enzalutamide. However, our understanding the effect these therapies on immune system in mCRPC patients remains limited. Here, we examined how enzalutamide treatment affects levels soluble mediators plasma circulating cells 44 patients. We found that baseline cytokines fibroblast growth factor (FGF),...
Preformed T-cell immune-sensitization should most likely impact allograft outcome during the initial period after kidney transplantation, since donor-specific memory T-cells may rapidly recognize alloantigens and activate effector immune response, which leads to rejection. However, precise time-frame in acute rejection is fundamentally triggered by preformed T cells rather than de novo activated naïve still be established. Here, alloreactive responses were evaluated using IFN-γ ELISPOT assay...
Ischemia-reperfusion occurs in a great many clinical settings and contributes to organ failure or dysfunction. CD154-CD40 signaling leukocyte–endothelial cell interactions T-cell activation facilitates tissue inflammation injury. Here we tested siRNA anti-CD40 rodent warm cold ischemia models check the therapeutic efficacy anti-inflammatory outcome of vivo gene silencing. In model different doses were used, resulting clear renal function improvement structural renoprotective effect. Renal...
Chronic immunosuppression promotes nonmelanocytic squamous cell carcinoma (SCC) after kidney transplantation. Adaptive and innate immunity play a key role controlling tumor growth are influenced by different immunosuppressive agents. We hypothesized that functional impairment of tumor-specific T responses due to calcineurin inhibitors (CNI) could contribute SCC development, whereas conversion mammalian target rapamycin (mTOR-i) recover this protective immune response.Peripheral against main...
2 Background: The REDEEM (Reduction by Dutasteride of Clinical Progression Events in Expectant Management Prostate Cancer) study tested whether dutasteride controlled growth existing low risk, localized prostate cancer (PCa) and hence reduced the need for aggressive therapy men followed with active surveillance. Methods: 302 men, aged 48–82, PSA <11 ng/ml, Gleason score ≤6 PCa (≤3 cores positive, <50% any core positive) were randomized to or placebo 3 years. Repeat 12-core biopsies...
Abstract CMV infection is a significant complication after solid organ transplantation. We used single cell TCR αβ sequencing to determine how memory inflation impacts clonality and diversity of the CMV-responsive CD8 CD4 T repertoire in first year transplantation human subjects. observed but no changes clonal diversity, indicating homeostatic stability clones. In contrast, was diverse stable over time, with evidence expansion. identified shared CDR3 motifs among patients public CMV-specific...
Memory/effector T cells play a major role in allograft rejection, however, clinical assessment of anti-donor cellular sensitization is not currently being evaluated human transplantation. In this study, pre-transplant donor-specific memory/efector T-cell responses were retrospectively using the IFNγ Elispot assay, group 150 consecutive kidney transplant recipients to assess impact before transplantation on outcome. Most patients with high alloreactivity received allografts from cadaveric...
CMV is a major infectious complication following solid organ transplantation. Reactivation of leads to memory inflation, process in which CD8 T cells expand over time. Memory inflation associated with specific changes cell function, including increased oligoclonality, decreased cytokine production, and terminal differentiation. To address whether during the first year after transplantation human subjects alters differentiation we employed single-cell-matched TCRαβ targeted gene expression...
Human CMV infection (hCMVi) is still a major complication after renal transplantation, challenging both graft and patient survival. Nowadays, the immune-risk stratification for hCMVi in Organ solid transplantation exclusively based on hCMV-specific antibody (IgG) serostatus of donor (D) transplant recipient (R), even though CMV-specific T cell responses to viral antigens such as pp65 ie1, have been demonstrated play key role at controlling virus replication. We hypothesized that IgG negative...
Antiretroviral treatment in the last decade of 20th century marked turning tide battle against AIDS.Inspired by patients and staff Positive Care Clinic Atherton, California, artist David Putnam created a triptych to be displayed clinic, illustrating new hope for those affected HIV.The panel on left, centerpiece original triptych, depicts protease inhibitors (blue dots) pitted HIV with its enzyme complex (in black).In right panel, bright yellow background expresses triumph therapy as virus is...