A5045126878- Endoplasmic Reticulum Stress and Disease
- Biochemical Analysis and Sensing Techniques
- Olfactory and Sensory Function Studies
- Ion Channels and Receptors
- Toxin Mechanisms and Immunotoxins
- Cellular transport and secretion
- RNA and protein synthesis mechanisms
- Neurobiology and Insect Physiology Research
- Clostridium difficile and Clostridium perfringens research
- Regulation of Appetite and Obesity
- Pancreatic function and diabetes
- Lipid Membrane Structure and Behavior
- Plant Reproductive Biology
- Advanced Chemical Sensor Technologies
- Protein Structure and Dynamics
- Neuroscience and Neuropharmacology Research
- RNA Interference and Gene Delivery
- Mycobacterium research and diagnosis
- RNA regulation and disease
Saarland University
2015-2023
Constant exposure of the airways to inhaled pathogens requires efficient early immune responses protecting against infections. How bacteria on epithelial surface are detected and first-line protective mechanisms initiated not well understood. We have recently shown that tracheal brush cells (BCs) express functional taste receptors. Here we report bitter signaling in murine BCs induces neurogenic inflammation. demonstrate BC stimulates adjacent sensory nerve endings trachea release...
Highlights•ES1, ES2, and ES24 deplete Ca2+ in ER•ESR35 ES47 do not affect cellular homeostasis•The most potent eeyarestatin, ES24, comprises only the 5-nitrofuran domain•ES1 target Sec61 complexes ERSummaryEeyarestatin 1 (ES1) inhibits p97-dependent protein degradation, Sec61-dependent translocation into endoplasmic reticulum (ER), vesicular transport within endomembrane system. Here, we show that ES1 impairs homeostasis by enhancing leakage from mammalian ER. A comparison of various analogs...
Abstract The median eminence (ME) is a circumventricular organ at the base of brain that controls body homeostasis. Tanycytes are its specialized glial cells constitute ventricular walls and regulate different physiological states, however individual signaling pathways in these incompletely understood. Here, we identify functional tanycyte subpopulation expresses key taste transduction genes including bitter receptors, G protein gustducin gustatory ion channel TRPM5 (M5). M5 tanycytes have...
Thapsigargin (TG) inhibits the sarco/endoplasmic reticulum Ca 2+ ATPase (SERCA) pump and, when applied acutely, it initiates a mobilisation that begins with loss of from endoplasmic (ER) and culminates store-operated entry (SOCE) extracellular space. Using popular model cell line HEK-293, we quantified TG-induced changes in cytosolic ER levels using FURA-2 FRET-based sensor D1ER, respectively. Our analysis predicts an leak 5–6 µM⋅s −1 for typical basal level 335–407 µM HEK-293 cells. The...
The Mycobacterium ulcerans exotoxin, mycolactone, is an inhibitor of co-translational translocation via the Sec61 complex. Mycolactone has previously been shown to bind to, and alter structure major translocon subunit Sec61α, change its interaction with ribosome nascent chain complexes. In addition function in protein into ER, also plays a key role cellular Ca2+ homeostasis, acting as leak channel between endoplasmic reticulum (ER) cytosol. Here, we have analysed effect mycolactone on...
The endoplasmic reticulum (ER) is extensively remodelled during the development of professional secretory cells to cope with high protein production. Since ER principal Ca2+ store in cell, we characterised homeostasis NALM-6 and RPMI 8226 cells, which are commonly used as human pre-B antibody secreting plasma cell models, respectively. Expression levels Sec61 translocons corresponding Sec61-mediated leak from ER, storage capacity store-operated entry were significantly enlarged line. Using...
Chemosensory cues detected in the nose need to be integrated with hormonal status trigger appropriate behaviors, but neural circuits linking olfactory and endocrine system are insufficiently understood. Here, we characterize sensory neurons murine that respond pituitary hormone prolactin. Deletion of prolactin receptor these cells results impaired detection social odors blunts male preference females. The prolactin-responsive exhibit a distinctive projection pattern brain is similar across...
Gonadotropes in the anterior pituitary gland are essential for fertility and provide a functional link between brain gonads. To trigger ovulation, gonadotrope cells release massive amounts of luteinizing hormone (LH). The mechanism underlying this remains unclear. Here, we utilize mouse model expressing genetically encoded Ca2+ indicator exclusively gonadotropes to dissect intact pituitaries. We demonstrate that female exhibit state hyperexcitability during LH surge, resulting spontaneous...
Abstract The Mycobacterium ulcerans exotoxin, mycolactone, is an inhibitor of co-translational translocation via the Sec61 complex. Mycolactone has previously been shown to bind to, and alter structure of, major translocon subunit Sec61α, change its interaction with ribosome nascent chain complexes. In addition function in protein into ER, also plays a key role cellular Ca 2+ homeostasis, acting as leak channel between endoplasmic reticulum (ER) cytosol. Here, we have analysed effect...