A5076787073- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Plant Reproductive Biology
- Plant tissue culture and regeneration
- Photosynthetic Processes and Mechanisms
- Plant Gene Expression Analysis
- Plant nutrient uptake and metabolism
- SARS-CoV-2 and COVID-19 Research
- Bacteriophages and microbial interactions
- Genomics and Phylogenetic Studies
- CRISPR and Genetic Engineering
- SARS-CoV-2 detection and testing
- Viral gastroenteritis research and epidemiology
- Animal and Plant Science Education
- Animal Virus Infections Studies
- Plant-Microbe Interactions and Immunity
- T-cell and Retrovirus Studies
- Microbial bioremediation and biosurfactants
- Diverse Educational Innovations Studies
- Enzyme Structure and Function
- Mycobacterium research and diagnosis
- Cellular Mechanics and Interactions
- Seed Germination and Physiology
- Environmental remediation with nanomaterials
- Genetic Syndromes and Imprinting
Howard University
2008-2023
National Institute of Environmental Health Sciences
2006
National Institutes of Health
2006
University of Leicester
2006
Triangle
2006
University of North Carolina at Chapel Hill
2001-2004
University of Wisconsin–Madison
2001
The phytohormone abscisic acid (ABA) promotes plant water conservation by decreasing the apertures of stomatal pores in epidermis through which loss occurs. We found that Arabidopsis thaliana plants harboring transferred DNA insertional mutations sole prototypical heterotrimeric GTP-binding (G) protein α subunit gene, GPA1 , lack both ABA inhibition guard cell inward K + channels and pH-independent activation anion channels. Stomatal opening gpa1 is insensitive to ABA, rate from mutants...
The α subunit of a prototypical heterotrimeric GTP-binding protein (G protein), which is encoded by single gene ( GPA1 ) in Arabidopsis , modulator plant cell proliferation. gpa1 null mutants have reduced division aerial tissues throughout development. Inducible overexpression confers inducible ectopic division. synchronized BY-2 cells causes premature advance the nuclear cycle and appearance wall. Results from loss function expression activation indicate that this positive plants.
Abstract Plant cells respond to low concentrations of auxin by cell expansion, and at a slightly higher concentration, these divide. Previous work revealed that null mutants the α-subunit putative heterotrimeric G protein (GPA1) have reduced division. Here, we show this prototypical complex acts mechanistically controlling sensitivity toward Loss-of-function altered auxin-mediated division throughout development, especially during auxin-induced formation lateral adventitious root primordia....
To directly address the function of a putative auxin receptor designated ABP1, reverse genetic approach was taken to identify and characterize ABP1 mutant alleles in Arabidopsis. A homozygous null mutation confers embryo lethality. Null embryos develop normally until early stages globular but are unable make transition bilaterally symmetrical structure because cells fail elongate. Cell division also aberrant both suspensor proper. Antisense suppression tobacco causes slow proliferation...
Abstract Seed germination is regulated by many signals. We investigated the possible involvement of a heterotrimeric G protein complex in this signal regulation. Seeds that carry null mutation gene encoding alpha subunit Arabidopsis (GPA1) are 100-fold less responsive to gibberellic acid (GA), have increased sensitivity high levels Glc, and near-wild-type response abscisic ethylene, indicating GPA1 does not directly couple these signals control. ectopically expressingGPA1 at least...
Many nuclear hormones have physiological effects that are too rapid to be explained by changes in gene expression and often attributed unidentified or novel G protein-coupled receptors. Thyroid hormone is essential for normal human brain development, but the molecular mechanisms responsible its remain identified. Here, we present direct evidence potassium channel stimulation a rat pituitary cell line (GH 4 C 1 ) receptor thyroid hormone, TRβ, acting rapidly at plasma membrane through...
The receptor for activated C-kinase 1 (RACK1) is a highly conserved WD40 repeat scaffold protein found in wide range of eukaryotic species from Chlamydymonas to plants and humans. In tissues higher mammals, RACK1 ubiquitously expressed has been implicated diverse signaling pathways involving neuropathology, cellular stress, translation, developmental processes. established itself as through physical interaction with myriad proteins ranging kinases, phosphatases, ion channels, membrane...
Scaffold proteins are known to regulate important cellular processes by interacting with multiple modulate molecular responses. RACK1 (Receptor for Activated C Kinase 1) is a WD-40 type scaffold protein, conserved in eukaryotes, from Chlamydymonas plants and humans, expresses ubiquitously plays regulatory roles diverse signal transduction stress response pathways. Here we present the use of Arabidopsis RACK1A, predominant isoform 3-member family, as bait screen split-ubiquitin based cDNA...
Scaffold protein Receptor for Activated C Kinase 1 (RACK1) is a negative regulator of plant stress hormone - abscisic acid (ABA) mediated pathways. RACK1 has been reported to regulate global miRNA biogenesis pathway in C. elegans, humans, and Arabidopsis. regulates different steps stability response stimuli plants. miR393s implicated salt through an antagonistic between the ABA-mediated growth auxin. Specifically, known auxin receptor clade transcripts TIR1/AFB2 are target miR393s. By...
// Hemayet Ullah 1 , Wangheng Hou 2 Sivanesan Dakshanamurthy 3 and Qiyi Tang Department of Biology, Howard University, Washington, DC 20059, USA Microbiology, University College Medicine, Oncology, Clinical Experimental Therapeutics Program, Lombardi Comprehensive Cancer Center, Georgetown Medical 20057, Correspondence to: Dakshanamurthy, email: sd233@georgetown.edu Tang, qiyi.tang@howard.edu Ullah, hullah@howard.edu Keywords: host targeted antiviral (HTA); herpes simplex virus (HSV);...
Abstract Population growth and climate change will impact food security potentially exacerbate the environmental toll that agriculture has taken on our planet. These existential concerns demand a passionate, interdisciplinary, diverse community of plant science professionals is trained during 21st century. Furthermore, societal trends question importance expert knowledge highlight need to better communicate value rigorous fundamental scientific exploration. Engaging students general public...
Scaffold proteins are known as important cellular regulators that can interact with multiple to modulate diverse signal transduction pathways. RACK1 (Receptor for Activated C Kinase 1) is a WD-40 type scaffold protein, conserved in eukaryotes, from Chlamydymonas plants and humans, plays regulatory roles stress response humans has been implicated myriads of neuropathological diseases including Alzheimer alcohol addictions. Model plant Arabidopsis thaliana genome maintains three different...
As energy sources and structural components, sugars are the central regulators of plant growth development. In addition to abundant natural in plants, more than 50 different kinds rare exist nature, several which show distinct roles Recently, one sugars, D-allose, an epimer D-glucose at C3, is found suppress hormone gibberellin (GA) signaling rice. Scaffold protein RACK1A model Arabidopsis implicated GA pathway as rack1a knockout mutants insensitivity GA-induced seed germination. Using...
The scaffold protein receptor for Activated C Kinase1 (RACK1) regulates multiple aspects of plants, including seed germination, growth, environmental stress responses, and flowering. Recent studies have revealed that RACK1 is associated with NADPH-dependent reactive oxygen species (ROS) signaling in plants. ROS, as a double-edged sword, can modulate several developmental pathways Thus, the resulting physiological consequences perturbing expression-induced ROS balance remain to be explored....
Receptor for activated C kinase 1 (RACK1) is WD-40 type scaffold protein, conserved in all eukaryote organisms. Many reports implicated RACK1 plant hormone signal transduction pathways including auxin and diverse stress signaling pathways; however, the precise molecular mechanism of its role not understood. Previously, a group small compounds targeting Arabidopsis RACK1A functional site-Tyr248 have been developed. Here, three different are used to elucidate mediated lateral root development....
Abstract: The widely conserved RACK1 protein is a WD-40 type scaffold that regulates diverse environmental stress signal transduction pathways. Arabidopsis RACK1A has been reported to interact with various proteins in salt and Light-Harvesting Complex (LHC) However, the mechanism of how contributes photosystem chlorophyll metabolism conditions remains elusive. Using T-DNA-mediated activation tagging transgenic rice (Oryza sativa L.) lines, we show leaves from RACK1B gene (OsRACK1B)...
The widely conserved Receptor for Activated C Kinase1 (RACK1) protein is a WD-40 type scaffold that regulates diverse environmental stress signal transduction pathways. Arabidopsis RACK1A has been reported to interact with various proteins in salt and Light-Harvesting Complex (LHC) However, the mechanism of how RACK1 contributes photosystem chlorophyll metabolism conditions remains elusive. In this study, using T-DNA-mediated activation tagging transgenic rice (Oryza sativa L.) lines, we...
The initial phase of the COVID-19 pandemic changed nature course delivery from largely in-person to exclusively remote, thus disrupting well-established pedagogy Genomics Education Partnership (GEP; https://www.thegep.org). However, our web-based research adapted well remote learning environment. As usual, students who engaged in GEP's Course-based Undergraduate Research Experience (CURE) received digital projects based on genetic information within assembled
Myeloperoxidase (MPO) is a heme peroxidase with microbicidal properties. MPO plays role in the host’s innate immunity by producing reactive oxygen species inside cell against foreign organisms. However, there little functional evidence linking missense mutations to human diseases. We utilized silico saturation mutagenesis generate and analyze effects of 10,811 potential on stability. Our results showed that ~71% destabilize MPO, ~8% stabilize protein. G402W, G402Y, G361W, G402F, G655Y would...
Genetic imprinting: the parent of origin?specific biased expression alleles is an important type epigenetic gene regulation in flowering plants and mammals. All imprinted genes show either maternal ? or paternal?specific mono?allelic expression. Considering that mammals shared a common ancestor more than one billion years ago, significant overlap potentially equally differences genomic imprinting mechanisms these two taxa are emerging. In plants, primarily ephemeral endosperm tissues seeds...