A5043366961- Reproductive Physiology in Livestock
- Ruminant Nutrition and Digestive Physiology
- Liver Disease Diagnosis and Treatment
- Adipose Tissue and Metabolism
- Cancer-related molecular mechanisms research
- Lipid metabolism and biosynthesis
- Genetic and phenotypic traits in livestock
- Milk Quality and Mastitis in Dairy Cows
- Effects of Environmental Stressors on Livestock
- Autophagy in Disease and Therapy
- Animal health and immunology
- Probiotics and Fermented Foods
- Birth, Development, and Health
- Sirtuins and Resveratrol in Medicine
- Diet and metabolism studies
- Cancer, Hypoxia, and Metabolism
- Digestive system and related health
- Alcohol Consumption and Health Effects
- Microbial infections and disease research
- Circular RNAs in diseases
- Reproductive System and Pregnancy
- Diet, Metabolism, and Disease
- Endoplasmic Reticulum Stress and Disease
- Infant Nutrition and Health
- Heme Oxygenase-1 and Carbon Monoxide
Anhui Medical University
2023-2025
Guangzhou Medical University
2024-2025
Lanzhou Petrochemical Polytechnic
2025
Qingzhou City People's Hospital
2025
Northeastern University
2020-2024
University of Illinois Urbana-Champaign
2019-2023
Nanjing Forestry University
2023
University of Michigan
2023
Urbana University
2019-2022
University of Illinois System
2020-2021
The effect of solvent-extracted cottonseed meal (SCSM) as a partial or total replacement fishmeal was studied in juvenile rainbow trout (Oncorhynchus mykiss). Six experimental diets SCSM0, SCSM25, SCSM50, SCSM75, SCSM75A and SCSMT, containing gradient SCSM 0, 152, 305, 465, 460 610 g kg−1 to replace 112.5, 225, 337.5, 337.5 450 g kg−1 protein were fed triplicate groups (initial body weight 39.2 ± 0.1 g) for 8 weeks. diet supplemented with lysine methionine, be similar SCSM0 trout. Faeces...
This study was conducted to establish a stable hypobaric hypoxia brain injury model. SD rats were randomly separated into control and model groups, placed outside or inside of chamber, respectively. Subsequent 24 h anoxic exposure, plasma superoxide dismutase (SOD), malondialdehyde (MDA), glutathione (GSH), oxidized (GSSG) lactate dehydrogenase (LDH) measured using commercial biochemical kits. Hematoxylin‑eosin (H&E), Nissl's terminal deoxynucleotidyl transferase dUTP nick end labeling...
Abstract Background We aimed to characterize the protective effects and molecular mechanisms of action a Saccharomyces cerevisiae fermentation product (NTK) in response mastitis challenge. Eighteen mid-lactation multiparous Holstein cows ( n = 9/group) were fed control diet (CON) or CON supplemented with 19 g/d NTK for 45 d (phase 1, P1) then infected right rear quarter 2500 CFU Streptococcus uberis 2, P2). After 36-h, mammary gland liver biopsies collected antibiotic treatment started until...
Methionine (Met) and arginine (Arg) regulate casein protein abundance through alterations in activity of the mechanistic target rapamycin complex 1 (mTORC1) signaling pathway. A potential role for circadian clock network on regulation synthesis, partly via mTORC1, has been highlighted non-ruminants. The main objective study was to determine ruminant mammary cells mRNA, phosphorylation status mTORC1-related upstream targets, proteins, kinase AMP-activated catalytic subunit alpha (AMPK)...
The quality of milk is inseparable from its components, and fatty acid content a key factor affecting the milk. In this study, miRNA mRNA profiles bovine mammary gland tissue during dry period peak lactation were determined through high-throughput sequencing. total, 72 miRNA–mRNA regulatory pathways screened, including miR-128/PPARGC1A pathways. miR-128 can directly target PPARGC1A inhibit expression. addition, study also observed that there was binding site in sequence circular RNA...
Ketosis is a serious metabolic disorder characterized by systemic and hepatic oxidative stress, inflammation, apoptosis, as well reduced milk yield. Because of the paucity data on mammary responses during ketosis, aim this study was to evaluate alterations in NF-κB signaling, NLRP3 inflammasome, caspase apoptotic pathways gland dairy cows with ketosis. Blood, tissue, samples were collected from healthy [Control, blood concentration β-hydroxybutyrate (BHB) <0.6 mM, n = 10] subclinical ketosis...
Milk lipids originate from cytoplasmic lipid droplets (LD) that are synthesized and secreted mammary epithelial cells by a unique membrane-envelopment process. Butyrophilin 1A1 (BTN1A1) is one of the membrane proteins surrounds LD, but its role in bovine droplet synthesis secretion not well known. The objective was to knockout BTN1A1 (BMEC) via CRISPR/Cas9 system evaluate LD formation, abundance lipogenic enzymes, content cell phospholipid (PL) species. Average diameter determined Oil Red O...
The store-operated Ca2+ entry (SOCE) moiety ORAI calcium release-activated modulator 1 (ORAI1) located in the endoplasmic reticulum (ER) participates key cellular functions such as protein folding, transport, and secretion, lipid metabolism. We used an vitro approach to test whether exogenous fatty acids alter ORAI1 signaling explore potential consequences on mitochondrial dysfunction ER stress. First, hepatocytes isolated from 4 healthy female calves (1 d old, 40–50 kg) were challenged with...
Abstract Background Bovine mammary epithelial cells after calving undergo serious metabolic challenges and oxidative stress both of which could compromise autophagy. Transcription factor EB (TFEB)-mediated autophagy is an important cytoprotective mechanism against stress. However, effects TFEB-mediated on the bovine remain unknown. Therefore, main aim study was to investigate role in experiencing Results H 2 O challenge cell MAC-T increased protein abundance LC3-II, number autophagosomes...
Abstract Background Previously we reported that inhibition of GPR17 prevents amyloid β 1–42 (Aβ 1-42 )-induced cognitive impairment in mice. However, the role on cognition is still largely unknown. Methods Herein, used a mouse model induced by lipopolysaccharide (LPS) to further investigate and its potential mechanism. The mice were pretreated with shRNA lentivirus cangrelor microinjection into dentate gyrus (DG) region hippocampus. After 21 days, LPS (0.25 mg/kg, i.p.) was administered for...
Ketosis in dairy cows is associated with body fat mobilization during the peripartal period. Sub-clinical and clinical ketosis arise more frequently that are overfed energy entire dry (last 50 to 45 days prior parturition) or close-up period ~ 28 prepartum).A retrospective analysis was performed on 12 from a larger cohort were fed higher-energy diet [1.54 Mcal/kg of matter (DM); 35.9% DM corn silage 13% ground corn] period, which 6 did not develop (OVE, 0.83 mmol/L plasma hydroxybutyrate;...
Cardamonin (CD), a naturally occurring chalcone derived from the Alpinia species, has been shown to exert antioxidant and anti-inflammatory activity, but its role in prevention of acetaminophen- (APAP-) induced hepatotoxicity remains elusive. The objective this study was determine protective effects CD against APAP-induced acute liver injury (ALI) underlying mechanisms. Wild-type or transcription factor nuclear erythroid 2-related 2- (NFE2L2-) deficient mice were treated with (50 100 mg/kg,...
Fatty acid composition plays a key role in regulating flavor and quality of milk. Therefore, order to improve milk quality, it is particularly important investigate regulatory mechanisms fatty metabolism. Circular RNAs (circRNAs) regulate expression genes associated with several biological processes including In this study, high-throughput sequencing was used detect differentially expressed bovine mammary tissue at early lactation peak lactation. Circ09863 profiles were influenced by the...
Abstract Periods of decreased feed intake may disrupt function the intestinal barrier. Feeding NutriTek® (NTK; Diamond V, Cedar Rapids, IA), a postbiotic from S. cerevisiae fermentation (SCFP), improved health and supported anti-inflammatory functions. We investigated effects feeding NTK to cows before during period restriction (FR) designed model periods barrier dysfunction. In total, 16 multiparous (97.1 ± 7.6 DIM; n = 8/group) were fed control diet (CON) or CON plus 19 g/d for 9 wk (Phase...
The growth of skeletal muscle relies on a delicate equilibrium between protein synthesis and degradation; however, how proteostasis is managed in the endoplasmic reticulum largely unknown. Here, we report that SEL1L-HRD1 (ER)-associated degradation (ERAD) complex, primary molecular machinery degrades misfolded proteins ER vital to maintain postnatal systemic energy balance. Myocyte-specific SEL1L deletion blunts hypertrophic phase growth, resulting net zero gain mass during this...