A5038038199- Nerve injury and regeneration
- Regulation of Appetite and Obesity
- Neurogenesis and neuroplasticity mechanisms
- Neuroscience and Neuropharmacology Research
- Adipose Tissue and Metabolism
- Neurotransmitter Receptor Influence on Behavior
- Eating Disorders and Behaviors
- Anesthesia and Neurotoxicity Research
- Pancreatic function and diabetes
- Treatment of Major Depression
- Neuroendocrine regulation and behavior
- Neuropeptides and Animal Physiology
- Sleep and Wakefulness Research
- Circadian rhythm and melatonin
- Biochemical Analysis and Sensing Techniques
- Autism Spectrum Disorder Research
- Stress Responses and Cortisol
- Hypothalamic control of reproductive hormones
- Rheumatoid Arthritis Research and Therapies
- Axon Guidance and Neuronal Signaling
- Hematopoietic Stem Cell Transplantation
- Immunotherapy and Immune Responses
- Fibroblast Growth Factor Research
- Bone health and treatments
- NF-κB Signaling Pathways
Tufts University
2013-2024
Universidad Autónoma del Estado de Morelos
2013
The University of Texas Southwestern Medical Center
2006
Whitehead Institute for Biomedical Research
1999-2005
Massachusetts Institute of Technology
2002
Harvard University
1990-1991
Howard Hughes Medical Institute
1990-1991
Dana-Farber Cancer Institute
1990
Mice experiencing repeated aggression develop a long-lasting aversion to social contact, which can be normalized by chronic, but not acute, administration of antidepressant. Using viral-mediated, mesolimbic dopamine pathway-specific knockdown brain-derived neurotrophic factor (BDNF), we showed that BDNF is required for the development this experience-dependent aversion. Gene profiling in nucleus accumbens indicates local obliterates most effects on gene expression within circuit, with...
Brain-derived neurotrophic factor has been associated previously with the regulation of food intake. To help elucidate role this neurotrophin in weight regulation, we have generated conditional mutants which brain-derived eliminated from brain after birth through use cre-loxP recombination system. were hyperactive exposure to stressors and had higher levels anxiety when evaluated light/dark exploration test. They also mature onset obesity characterized by a dramatic 80-150% increase body...
Brain-derived neurotrophic factor (BDNF) is involved in synaptic plasticity, neuronal differentiation and survival of neurons. Observations decreased serum BDNF levels patients with neuropsychiatric disorders have highlighted the potential as a biomarker, but so far there been no studies directly comparing blood to brain different species. We examined blood, serum, plasma brain-tissue three mammalian species: rat, pig, mouse, using an ELISA method. As control, we included analysis tissue...
Brain-derived neurotrophic factor (BDNF) and its receptor TrkB are expressed in several hypothalamic hindbrain nuclei involved regulating energy homeostasis, developmentally the adult animal. Their depletion during fetal or early postnatal periods when developmental processes still ongoing elicits hyperphagic behavior obesity mice. Whether BDNF is a chief element appetite control mature brain remains controversial. The required sources of this neurotrophin also unknown. We show that glucose...
Brain-derived neurotrophic factor (BDNF) and its receptor, TrkB, play prominent roles in food intake regulation through central mechanisms. However, the neural circuits underlying their anorexigenic effects remain largely unknown. We showed previously that selective BDNF depletion ventromedial hypothalamus (VMH) of mice resulted hyperphagic behavior obesity. Here, we sought to ascertain whether regulatory involved mesolimbic dopamine system, which mediates motivated reward-seeking behaviors...
Background Antidepressant drugs (ADs) have been shown to activate BDNF (brain-derived neurotrophic factor) receptor TrkB in the rodent brain but mechanism underlying this phenomenon remains unclear. ADs act as monoamine reuptake inhibitors and after prolonged treatments regulate bdnf mRNA levels indicating that monoamine-BDNF signaling AD-induced activation vivo. However, recent findings demonstrate Trk receptors can be transactivated independently of their neurotrophin ligands. Methodology...
Brain-derived neurotrophic factor has been associated previously with the regulation of food intake. To help elucidate role this neurotrophin in weight regulation, we have generated conditional mutants which brain-derived eliminated from brain after birth through use cre-loxP recombination system. were hyperactive exposure to stressors and had higher levels anxiety when evaluated light/dark exploration test. They also mature onset obesity characterized by a dramatic 80–150% increase body...
Catecholamine neurotransmitters are synthesized by hydroxylation of tyrosine to L-dihydroxyphenylalanine (L-Dopa) hydroxylase (TH). The elimination TH in both pigmented and albino mice described here, like TH-null reported previously (Kobayashi et al., 1995; Zhou 1995), demonstrates the unequivocal requirement for catecholamines during embryonic development. Although lack is fatal, embryos can be rescued administration catecholamine precursors pregnant dams. Once born, pups survive without...
Chronic opiate exposure induces numerous neurochemical adaptations in the noradrenergic system, including upregulation of cAMP-signaling pathway and increased expression tyrosine hydroxylase (TH), rate-limiting enzyme catecholamine biosynthesis. These are thought to compensate for opiate-mediated neuronal inhibition but also contribute physical dependence, withdrawal after abrupt cessation drug exposure. Little is known about molecules that regulate response opiates. Here we report locus...
A brief burst-suppressing isoflurane anesthesia has been shown to rapidly alleviate symptoms of depression in a subset patients, but the neurobiological basis these observations remains obscure. We show that single produces antidepressant-like behavioural effects learned helplessness paradigm and regulates molecular events implicated mechanism action rapid-acting antidepressant ketamine: activation brain-derived neurotrophic factor (BDNF) receptor TrkB, facilitation mammalian target...
Adiponectin (APN), the most abundant adipocyte-secreted adipokine, regulates energy homeostasis and exerts well-characterized insulin-sensitizing properties. The peripheral or central effects of APN regulating bone metabolism are beginning to be explored but still not clearly understood. In present study, we found that APN-knockout (APN-KO) mice fed a normal diet exhibited decreased trabecular structure mineralization increased marrow adiposity compared with wild-type (WT) mice....
The global rise in obesity and related health issues, such as type 2 diabetes cardiovascular disease, is alarming. Gaining a deeper insight into the central neural pathways mechanisms that regulate energy glucose homeostasis crucial for developing effective interventions to combat this debilitating condition. A significant body of evidence from studies humans rodents indicates brain-derived neurotrophic factor (BDNF) signaling plays key role regulating feeding, expenditure, glycemic control....
Brain-derived neurotrophic factor (BDNF) plays important roles in neuronal differentiation/survival, the regulation of food intake, and pathobiology obesity type 2 diabetes mellitus. BDNF its receptor are expressed osteoblasts chondrocyte. vitro has a positive effect on bone; whether central affects bone mass vivo is not known. We therefore examined energy use brain-targeted conditional knockout mice (Bdnf(2lox/2lox)/93). The deletion brain led to metabolic phenotype characterized by...
Brain-derived neurotrophic factor (BDNF) is a potent regulator of neuronal activity, neurogenesis, and depressive-like behaviors; however, downstream effectors by which BDNF exerts these varying actions remain to be determined. Here we reveal that induces long-lasting enhancements in the efficacy synaptic inhibition stabilizing γ2 subunit-containing GABA A receptors (GABA Rs) at cell surface, leading persistent reductions excitability. This effect dependent upon enhanced phosphorylation...
Mice lacking catecholamines die before birth, some with cardiovascular abnormalities. To investigate the role of in development, embryonic day 12.5 (E12.5) fetuses were cultured and heart rate monitored. Under optimal oxygenation, wild-type catecholamine-deficient had same initial (200–220 beats/min), which decreased by 15% during 50 min culture. During culture period, dropped their 35%. Hypoxia reduced 35–40% 20% utero, assessed echocardiography. However, exhibited greater hypoxia-induced...
Hematopoietic stem cells interact with a complex microenvironment both in vivo and vitro. In association this microenvironment, murine are maintained vitro for several months. Fibroblast-like stromal appear to be important components of the since laboratories have demonstrated that cloned cell lines support hematopoiesis The importance tissue origin such remains unknown, systematic generation from adult tissues has never been accomplished. addition, capacity reconstituting not examined. We...