A5064835735- T-cell and B-cell Immunology
- vaccines and immunoinformatics approaches
- SARS-CoV-2 and COVID-19 Research
- Single-cell and spatial transcriptomics
- Signaling Pathways in Disease
- Cancer Genomics and Diagnostics
- Influenza Virus Research Studies
- Immune Cell Function and Interaction
- Neuroinflammation and Neurodegeneration Mechanisms
- COVID-19 Clinical Research Studies
- CAR-T cell therapy research
- Immunotherapy and Immune Responses
National Institute on Aging
2022-2024
National Institutes of Health
2022-2024
Abstract The decline of CD8 + T cell functions contributes to deteriorating health with aging, but the mechanisms that underlie this phenomenon are not well understood. We use single-cell RNA sequencing both cross-sectional and longitudinal samples assess how human heterogeneity transcriptomes change over nine decades life. Eleven subpopulations cells their dynamic changes age identified. Age-related in gene expression result from percentage expressing a given transcript, quantitative...
Abstract The resolution of SARS-CoV-2 replication hinges on cell-mediated immunity, wherein CD8 + T cells play a vital role. Nonetheless, the characterization specificity and TCR composition targeting non-spike protein before after infection remains incomplete. Here, we analyzed recognizing six epitopes from nucleocapsid (N) found that slightly increased frequencies N-recognizing but significantly enhanced activation-induced proliferation compared to uninfected donors. N-specific their...
T cell activation is a tightly controlled process involving both positive and negative regulators. The precise mechanisms governing the regulators in proliferation remain incompletely understood. Here, we report that homeodomain-only protein (HOPX), homeodomain-containing protein, its most abundant isoform
Abstract Influenza infection remains a leading cause of death among older adults worldwide. While vaccines offer an effective means reducing the severity influenza, their efficacy reduces in this demographic. To gain insight into adaptive immune response, we conducted analysis antigen-specific CD8+ T cells before and after administration high-dose seasonal influenza vaccine (Fluzone) over six seasons (2014, 2018, 2019, 2021, 2022, 2023) cohort healthy (n=37, M=18, F=19, aged 72-92, spanning...
Abstract Somatic mutation is a contributing factor for cancer and aging. DNA-based single-cell detection methods have been developed, but are hindered by the limited number of cells examined as well errors from PCR amplification sequencing methods. Here, we developed UMI-based scRNAseq (USCMD) method that allows examination up to 10,000 per sample with reduced errors, which also filters potential RNA polymerase II. We applied USCMD analyze data human CD8 +T collected 8 donors 2 visits over...
Abstract COVID-19, an infectious disease caused by SARS-CoV-2, has given rise to the current global pandemic. Despite number of infections reaching over 600 million people worldwide, CD8 +T cells against SARS-CoV-2 in COVID-19 convalescent, vaccinated, and unexposed adults have not been fully characterized. Here, we report frequency, phenotype, vitro expansion capacity circulating recognizing 10 epitopes from spike (S) protein. Through multi-color flow cytometry using antigen-specific...
Abstract COVID-19, an infectious disease caused by SARS-CoV-2, has given rise to the current global pandemic. Despite number of infections nearing 300 million people worldwide, CD8+ T cells against SARS-CoV-2 in COVID-19 convalescent, vaccinated, and healthy adults their changes with age have not been fully characterized. Here, we report frequency, phenotype, vitro expansion capacity circulating recognizing twenty-two epitopes from five proteins (S, N, M, ORF1ab, ORF3a). Through multi-color...