A5013632231- vaccines and immunoinformatics approaches
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Immunotherapy and Immune Responses
- Genetics, Bioinformatics, and Biomedical Research
- Single-cell and spatial transcriptomics
- T-cell and B-cell Immunology
- Zebrafish Biomedical Research Applications
National Institute on Aging
2022-2023
University of Washington
2021
Seattle University
2021
Abstract The resolution of SARS-CoV-2 replication hinges on cell-mediated immunity, wherein CD8 + T cells play a vital role. Nonetheless, the characterization specificity and TCR composition targeting non-spike protein before after infection remains incomplete. Here, we analyzed recognizing six epitopes from nucleocapsid (N) found that slightly increased frequencies N-recognizing but significantly enhanced activation-induced proliferation compared to uninfected donors. N-specific their...
Abstract COVID-19, caused by SARS-CoV-2 infection, sparked an ongoing global pandemic. While cell-mediated immunity is crucial in mitigating the severity and eradicating viral infections, a comprehensive characterization of CD8+ T cell antigen-specificity, frequency, proliferative capacity, TCR sequences against both COVID-19 vaccinated recovered adults remains incomplete. In this study, we embark on longitudinal analysis circulating cells targeting 10 epitopes from spike (S) protein across...
Abstract COVID-19, an infectious disease caused by SARS-CoV-2, has given rise to the current global pandemic. Despite number of infections reaching over 600 million people worldwide, CD8 +T cells against SARS-CoV-2 in COVID-19 convalescent, vaccinated, and unexposed adults have not been fully characterized. Here, we report frequency, phenotype, vitro expansion capacity circulating recognizing 10 epitopes from spike (S) protein. Through multi-color flow cytometry using antigen-specific...
Abstract COVID-19, an infectious disease caused by SARS-CoV-2, has given rise to the current global pandemic. Despite number of infections nearing 300 million people worldwide, CD8+ T cells against SARS-CoV-2 in COVID-19 convalescent, vaccinated, and healthy adults their changes with age have not been fully characterized. Here, we report frequency, phenotype, vitro expansion capacity circulating recognizing twenty-two epitopes from five proteins (S, N, M, ORF1ab, ORF3a). Through multi-color...
Abstract COVID-19, an infectious disease caused by SARS-CoV-2, has the current global pandemic. Despite number of infections nearing 100 million people worldwide, CD8+ T cells against SARS-CoV-2 in healthy adults as well convalescent COVID-19 patients are not fully characterized. Here, we report analysis frequency and phenotype circulating recognizing seventeen epitopes from three proteins (S, N, M) HLA-A2+ multi-color flow cytometry with antigen-specific tetramers. Through 65 ranging...