A5045318991- Fungal and yeast genetics research
- Biofuel production and bioconversion
- Viral Infectious Diseases and Gene Expression in Insects
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Production and Characterization
- Education Practices and Challenges
- Neurological disorders and treatments
- Educational Methods and Analysis
- Botulinum Toxin and Related Neurological Disorders
- Parasites and Host Interactions
- Values and Moral Education
- Transgenic Plants and Applications
- Protein purification and stability
- Peptidase Inhibition and Analysis
- Protein Hydrolysis and Bioactive Peptides
- Parasite Biology and Host Interactions
- vaccines and immunoinformatics approaches
- Monoclonal and Polyclonal Antibodies Research
- Proteins in Food Systems
- Agricultural and Rural Development Research
- Insect Resistance and Genetics
- Biocrusts and Microbial Ecology
- Pancreatic function and diabetes
- Microbial Metabolites in Food Biotechnology
- Algal biology and biofuel production
Izmir University
2019-2025
Akdeniz University
2010-2025
Akdeniz University Hospital
2020
University of Nebraska–Lincoln
2000-2011
It was observed that during fermentative production of recombinant ovine interferon-τ (r-oIFN-τ) in Pichia pastoris, a secreted protein, the protein degraded increasingly after 48 h induction and rate degradation increased towards end fermentation at 72 h, when stopped. Proteases, whose primary source vacuoles, found increasing levels cytoplasm broth reached maximal values batch completed h. Protease various cell fractions as well culture supernatant were lower glycerol used carbon instead...
A potential vaccine candidate, Necator americanus secretory protein (Na-ASP1), against hookworm infections, has been expressed in Pichia pastoris. Na-ASP1, a 45 kDa containing 20 cysteines, was directed outside the cell by fusing to preprosequence of alpha-mating factor Saccharomyces cerevisiae. Most produced single copy clones secreted cell. However, increasing gene number Na-ASP1 P. pastoris saturated capacity and therefore, decreased amount harboring multiple copies gene. Overexpression...
ABSTRACT Low‐cost and safe vaccines are needed to fill the vaccine inequity gap for future pandemics. Pichia pastoris is an ideal expression system recombinant protein production due its cost‐effective easy‐to‐scale‐up process. Here, we developed a next‐generation SARS‐CoV2 Omicron BA.1‐based candidate expressed in P. . The receptor binding domain of BA.1 spike (RBD‐Omicron) was produced at 0.35 g/L supernatant. With 60% recovery after two‐step purification, RBD‐Omicron showed 99% purity....
Pontryagin's Maximum Principle has been applied for optimization of secreted proteins from Pichia pastoris fed-batch fermentation. The objective this work is to maximize the total accumulated product per unit operation time under different given conditions and system constraints. To obtain optimal solutions, an automated curve-fitting software, Table Curve 2D, was employed construct necessary mathematical models solve complicated functions. In solution processes, end glycerol batch phase...
Abstract Recombinant protein-based SARS-CoV-2 vaccines are needed to fill the vaccine equity gap. Because protein-subunit based easier and cheaper produce do not require special storage/transportation conditions, they suitable for low-/middle-income countries. Here, we report our development studies with receptor binding domain of Delta Plus strain (RBD-DP) which caused increased hospitalizations compared other variants. First, expressed RBD-DP in Pichia pastoris yeast system upscaled it a...
Yeast expression systems with altered N-glycosylation are now available to produce glycoproteins homogenous, defined N-glycans. However, data on the behaviour of these strains in high cell density cultivation scarce. Here, we report cultivations under controlled specific growth rate a GlycoSwitch-Man5 Pichia pastoris strain producing Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) at levels (hundreds milligrams per liter). We demonstrate that homogenous Man5GlcNAc2 secreted...
A network of one-dimensional (1D) Au nanoparticle necklaces is synthesized and shown to exhibit electronic switching, that is, gating, by the metabolic activity yeast cells deposited on structure. Without cells, exhibits Coulomb blockade effect at room temperature with a sharp threshold voltage, VT ∼0.45 V, which corresponds switching energy ∼20 kT. Although enhancement in from ∼70 mV for single (10 nm) particle >1 V well-known 2D array, uniqueness topology relatively weak dependence leads...
Proteins with internal repeats are highly conserved among budding yeasts. In this study, the isolation of two proteins (PIR) genes, i.e. PpPIR1 and PpPIR2, from methylotrophic yeast Pichia pastoris has been reported. The PIR1 PIR2 genes' open reading frames were found to contain 1068 972 bases, respectively. sequence homology search showed a homologous repeat PIR block (SQIGDGQIQATT) in both proteins. was present eight times PpPir1p four PpPir2p Both glutamine (Q) aspartic acid (D) repeated...