A5084751225- Monoclonal and Polyclonal Antibodies Research
- Protein purification and stability
- Glycosylation and Glycoproteins Research
- Enzyme Structure and Function
- Protein Structure and Dynamics
- Metabolism and Genetic Disorders
- Computational Drug Discovery Methods
- RNA and protein synthesis mechanisms
- Corneal surgery and disorders
- SARS-CoV-2 and COVID-19 Research
- Biochemical and Molecular Research
- Peptidase Inhibition and Analysis
- Galectins and Cancer Biology
- vaccines and immunoinformatics approaches
- Glaucoma and retinal disorders
- Amino Acid Enzymes and Metabolism
- Alzheimer's disease research and treatments
- Corneal Surgery and Treatments
- Machine Learning in Bioinformatics
- Plant biochemistry and biosynthesis
- Protease and Inhibitor Mechanisms
- Retinal Development and Disorders
- Bacterial Genetics and Biotechnology
- Biochemical and Structural Characterization
- Genomics, phytochemicals, and oxidative stress
Izmir University
2019-2025
Lusaka Apex Medical University
2023-2024
Rensselaer Polytechnic Institute
2019-2020
Dokuz Eylül University
2020
University of Atlanta
2018
Georgia Institute of Technology
2014-2016
Koç University
2010
ABSTRACT Low‐cost and safe vaccines are needed to fill the vaccine inequity gap for future pandemics. Pichia pastoris is an ideal expression system recombinant protein production due its cost‐effective easy‐to‐scale‐up process. Here, we developed a next‐generation SARS‐CoV2 Omicron BA.1‐based candidate expressed in P. . The receptor binding domain of BA.1 spike (RBD‐Omicron) was produced at 0.35 g/L supernatant. With 60% recovery after two‐step purification, RBD‐Omicron showed 99% purity....
Abstract Recombinant protein-based SARS-CoV-2 vaccines are needed to fill the vaccine equity gap. Because protein-subunit based easier and cheaper produce do not require special storage/transportation conditions, they suitable for low-/middle-income countries. Here, we report our development studies with receptor binding domain of Delta Plus strain (RBD-DP) which caused increased hospitalizations compared other variants. First, expressed RBD-DP in Pichia pastoris yeast system upscaled it a...
Abstract Background PDZ domain is a well-conserved, structural protein found in hundreds of signaling proteins that are otherwise unrelated. domains can bind to the C-terminal peptides different and act as glue, clustering complexes together, targeting specific routing these pathways. These classified into classes I, II III, depending on their binding partners nature bonds formed. Binding specificities very crucial order understand complexity It still an open question how recognize partners....
Abstract Single chain antibody fragments (scFvs) are favored in diagnostic and therapeutic fields thanks to their small size the availability of various engineering approaches. Linker between variable heavy (V H ) light L chains scFv covalently links these domains it can affect scFv’s bio-physical/chemical properties vivo activity. Thus, linker design is important for a successful construction, flexible linkers preferred proper pairing V –V . The flexibility determined by length sequence...
Phage display is one of the most widely used technology for antibody discovery and engineering. Number therapeutic antibodies derived from phage increases rapidly due to its ease use ability control sequence information. Although there are numerous candidates as promising therapeutics, them fail at later stages development undesired biophysical properties. Antibody with poor properties should be prevented or improved in early phases minimize enormous loss time resources. In this study, we...
The number of therapeutic antibodies in preclinical, clinical, or approved phases has been increasing exponentially, mostly due to their known successes. Development antibody engineering methods substantially hastened the development antibodies. A variety protein techniques can be applied improve afinity and/or biophysical properties such as solubility and stability. Antibody fragments (where all some parts constant regions are eliminated while essential antigen binding region is preserved)...
Crystallization chaperones are attracting increasing interest as a route to crystal growth and structure elucidation of difficult targets such membrane proteins. While strategies date have typically employed protein-specific chaperones, peptide-specific chaperone crystallize multiple cognate peptide epitope-containing client proteins is envisioned. This would eliminate the target-specific chaperone-production step streamline co-crystallization process. Previously, protein engineering...
Matrix metalloproteinases (MMPs) are endopeptidases that responsible for the degradation of several components extracellular matrix (ECM) and some non-ECM proteins. MMPs subdivided into 6 groups according to their structure substrate specificity: collagenases, gelatinases, membrane-type MMPs, stromelysins, matrilizines. Collagenases important proteolytic tools during ECM remodeling, tissue regeneration, organ development. especially have roles in ocular processes such as retinal neurogenesis...
Macular corneal dystrophy (MCD) is a rare congenital disease caused by mutations in the carbohydrate sulfotransferase 6 (chst6) gene. Patients suffer from opaque aggregates cornea leading to bilateral progressive vision loss 4th decade of life. Corneal transplantation only available treatment, which invasive, not every patient and recurrence symptoms common. Keratocytes express chst6 gene, encodes golgi enzyme that essential for sulfation keratan sulfate proteoglycans (KSPG). The KS leads...
Abstract Checkpoint inhibitors are widely recognized immunotherapeutic drugs, known for their effectiveness in treating various cancers. Atezolizumab, targeting the immune checkpoint programmed death‐ligand 1, is successfully used to treat several types of Atezolizumab a potential biosimilar candidate due its huge success clinic but there no literature on production process mammalian cells. In this study, we generated monoclonal cell line derived from recombinant Chinese hamster ovary DG44...
Chemical details of intramembrane proteolysis remain elusive despite its prevalence throughout biology. We developed a FRET peptide assay for the aspartyl protease (IAP) from Methanoculleus marisnigri JR1 in combination with quantitative mass spectrometry cleavage site analysis. IAP can hydrolyze angiotensinogen sequence, substrate soluble renin, at predominant cut site, His-Thr. Turnover is slow (min(-1) × 10(-3)), affinity and Michaelis constant (Km) values are low micromolar range, both...
Abstract Sensitive detection of protein aggregates is important for evaluating the quality biopharmaceuticals and detecting misfolded proteins in several neurodegenerative diseases. However, it challenging to detect extremely low concentrations (<10 ppm) aggregated presence high soluble protein. Glucagon, a peptide hormone used treatment extreme hypoglycemia, aggregation‐prone forms amyloid fibrils. Detection glucagon fibrils using conformation‐specific antibodies an attractive approach...
Understanding the determinants of antibody specificity is one challenging tasks in development. Monospecific antibodies are still dominant approved therapeutics but there a significant body work to show that multispecific can increase overall therapeutic effect. Dual-specific or "Two-in-One" bind two different antigens separately with same antigen-binding site as opposed bispecifics, which simultaneously through separate units. These nonstandard dual-specific were recently shown be promising...
Protein crystallization is dependent upon, and sensitive to, the intermolecular contacts that assist in ordering proteins into a three-dimensional lattice. Here we used protein engineering mutagenesis to affect of single chain antibody fragments (scFvs) recognize EE epitope (EYMPME) with high affinity. These hypercrystallizable scFvs are under development difficult proteins, such as membrane forming crystals, by acting chaperones. Guided analyses crystal lattice contacts, two...
Background/aim: In recent years, single-domain antibodies, also known as nanobodies, have emerged an alternative to full immunoglobulin Gs (IgGs), due their various advantages including increased solubility, faster clearance, and cheaper production. Nanobodies are generally derived from the variable domain of camelid heavy-chain-only (hcIgGs). Due high sequence homology between heavy chains camelids (VHHs) humans (VHs), hcIgGs ideal candidates for nanobody development. However, further...
Biologics such as peptides and proteins possess a number of attractive attributes that make them particularly valuable therapeutics, including their high activity, specificity, low toxicity. However, one the key challenges associated with this class drugs is propensity to aggregate. Given safety immunogenicity concerns related polypeptide aggregates, it important sensitively detect aggregates in therapeutic drug formulations part quality control process. Here, we report development...
The carbohydrate sulfotransferase 6 ( chst6 ) gene is linked to macular corneal dystrophy (MCD), a rare disease that leads bilateral blindness due the accumulation of opaque aggregates in stroma. encodes for keratan sulfate proteoglycan (KSPG) specific sulfotransferase. MCD patients lose sulfated KSPGs (cKS) cornea and serum. significance serum cKS loss has not been understood. Zebrafish structure similar humans it contains high levels Here, zebrafish shown be expressed head structures...
Proteins are used in many areas including diagnostic and therapeutical applications. Screening protein stability is an essential step for production, pharmacokinetic/pharmacodynamic studies, determination of storage conditions. Instability proteins can cause serious problems such as activity loss unexpected adverse effects, so sensitive reliable methods measurement crucial. There several “gold-standard” tests differential scanning calorimetry (DSC), but they usually not suitable...