A5002789122- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- Viral Infections and Immunology Research
- Hepatitis Viruses Studies and Epidemiology
- Virus-based gene therapy research
- Congenital Anomalies and Fetal Surgery
- Advanced Thermodynamic Systems and Engines
- Respiratory viral infections research
- Child Nutrition and Water Access
- interferon and immune responses
- Poverty, Education, and Child Welfare
- Food Security and Health in Diverse Populations
- Scientific Computing and Data Management
- scientometrics and bibliometrics research
- Vector-Borne Animal Diseases
- Research Data Management Practices
- Urban and Rural Development Challenges
- Salmonella and Campylobacter epidemiology
- Global Maternal and Child Health
Noguchi Memorial Institute for Medical Research
2013-2024
University of Ghana
2013-2024
Indiana University Bloomington
2023-2024
Nagasaki University
2015-2024
Group A rotaviruses (RVAs) are the most important etiological agent of acute gastroenteritis in children <5 years age worldwide. The monovalent rotavirus vaccine Rotarix was introduced into national Expanded Programme on Immunization (EPI) Ghana May 2012. However, there is a paucity genetic and phylogenetic data complete genomes human RVAs circulation pre-vaccine introduction. common bovine VP7 genotype G8 has been sporadically detected Ghanaian children, usually combination with VP4 P[6]....
Rotaviruses are a significant cause of severe, potentially life-threatening gastroenteritis in infants and the young many economically important animals. Although vaccines against porcine rotavirus exist, both live oral inactivated, their effectiveness preventing is less than ideal. Thus, there need for development new generations vaccines. The Ohio State University (OSU) strain represents Rotavirus A species with G5P[7] genotype, genotype most frequently associated disease piglets. Using...
Open Science is encouraged by the European Union and many other political scientific institutions. However, practice proving slow to change. We propose, as early career researchers, that it our task change research into open commit principles.
Exposure to fecal contamination in public areas, especially dense, urban environments, may significantly contribute enteric infection risk. This study examined associations between sanitation and environments four low-income neighborhoods Accra, Ghana. Soil (n = 72) open drain 90) samples were tested for E. coli, adenovirus, norovirus. Sanitation facilities surveyed households 793) categorized by onsite sludge containment ("contained" vs. "uncontained") using previous Joint Monitoring...
Rotavirus A (RVA) strains, a leading cause of severe gastroenteritis in children worldwide, commonly possess the Wa or DS-1 genotype constellations. During hospital-based study conducted Hanoi, Vietnam, 2012-2013 rotavirus season, G1P[8] strains with virtually identical short RNA migration pattern were detected 20 (14%) 141 rotavirus-positive samples. Two representatives these shown by whole-genome sequencing to be double-gene reassortants possessing constellation...
ABSTRACT Rotaviruses are a significant cause of severe, potentially life-threatening gastroenteritis in the young many economically important animals. Although vaccines against porcine rotavirus exist, both live oral and inactivated, their effectiveness preventing is less than ideal. Thus, need remains for development new generations vaccines. The Ohio State University (OSU) strain represents Rotavirus A species with G5P[7] genotype, genotype most frequently associated disease piglets. Using...
Recent increase in the detection of unusual G1P[8], G3P[8], G8P[8], and G9P[4] Rotavirus A (RVA) strains bearing DS-1-like constellation non-G, non-P genes (hereafter referred to as genotype 2 backbone) requires better understanding their evolutionary relationship. However, within a genotype, there is lack consensus lineage designation framework set common sequences that can serve references. Phylogenetic analyses were carried out on over 8,500 RVA systematically retrieved from rotavirus...
Understanding the epidemiology of human norovirus infection in children within Ghana and entire sub-Saharan African region, where future vaccines would have greatest impact, is essential. We analyzed 1337 diarrheic stool samples collected from <5 years January 2008 to December 2017 found 485 (36.2%) shedding virus. GII.4 (54.1%), GII.3 (7.7%), GII.6 (5.3%), GII.17 (4.7%), GII.5 (4.7%) were most common genotypes. Although remained predominant capsid genotype throughout study period, an...
Rotavirus A (RVA) causes acute diarrhea in children as well animals. As part of a cross-sectional study less than 5 years age hospitalized for Vietnam during 15-month period (2007-2008), 322 (43.5%) 741 fecal specimens contained RVA with 92% either G1P[8] or G3P[8]. This was undertaken to further characterize strains that remained untypeable complete the G and P genotypes rotavirus-positive specimens. While 307 (95.3%) possessed common human genotypes: (45.0%), G2P[4] (2.8%), G3P[8] (46.9%),...
The live oral rotavirus RV1 (Rotarix) vaccine is formulated from the human G1P[8] RIX4414 virus. Based on sequences, T7 expression plasmids were constructed that supported recovery of recombinant RIX4414-like viruses by reverse genetics. These will advance study vaccine, possibly allowing improvements to its efficacy.
Noroviruses, an important cause of diarrhoea in humans, are genetically diverse. The recent norovirus seasons recorded the emergence new recombinants capsid and polymerase genotypes, with a global dominance GII.Pe_GII.4 Sydney 2012 GII.P17_GII.17 Asian countries. However, number papers reporting distribution both genotypes circulating among children is scarce, none from Vietnam. This study described noroviruses Vietnamese using stool specimens obtained under World Health Organization...
In crowded urban settlements in low-income countries, many households rely on shared sanitation facilities. Shared facilities are not currently considered "improved sanitation" because of concerns about whether hygiene conditions sufficiently protect users from the feces others. Prevention fecal exposure at a latrine is only one aspect sanitary safety. Ensuring consistent use latrines for disposal, especially child feces, required to reduce contamination and communities. Household crowding...
Diarrheal diseases cause significant morbidity and mortality worldwide, particularly in children under age five. Most of the 2.5 million annual diarrhea-related deaths occur developing countries, where access to treatment is limited by geographic, cultural knowledge-based factors. Noroviruses, leading diarrhea adults, are second viral etiology behind rotaviruses, causing a roughly estimated 218,000 child year. The recent introduction rotavirus vaccines throughout world may open path for...
Unusual human G6P[6] rotavirus A (RVA) strains have been reported sporadically in Europe and Africa, but how they evolved was not fully understood. The whole genome of a Ghanaian strain designated PML1965 (2012) analysed to understand it Africa learn its G6 VP7 gene related that rotaviruses artiodactyl origin. genotype constellation RVA/Human-wt/GHA/PML1965/2012/G6P[6] G6-P-[6]-I2-R2-C2-M2-A2-N2-T2-E2-H2. It shared sublineages with previously detected Italy all segments except the VP6 few...
ABSTRACT The binding of viral RNA to RIG-I-like receptors triggers the formation mitochondrial antiviral signaling (MAVS) protein aggregates critical for interferon (IFN) expression. Several rotavirus strains have been shown suppress IFN expression by inducing MAVS degradation. Relying on transient assays, previous studies reached different conclusions regarding identity responsible degradation, suggesting it was an activity capping enzyme VP3 or antagonist NSP1. Here, we used recombinant...
In 2010, the rare OP354-like P[8]b rotavirus subtype was detected in children less than 2 years old Ghana. this follow-up study, to provide insight into evolutionary history of genome Ghanaian strains RVA/Human-wt/GHA/GHDC949/2010/G9P[8] and RVA/Human-wt/GHA/GHM0094/2010/G9P[8] an infant a 7-month child hospitalised for acute gastroenteritis, we sequenced complete using both Sanger sequencing Illumina MiSeq technology followed by phylogenetic analysis near-full length sequences. Both...