A5055086956- Spine and Intervertebral Disc Pathology
- Musculoskeletal pain and rehabilitation
- Cervical and Thoracic Myelopathy
- TGF-β signaling in diseases
- Bone Metabolism and Diseases
- Spinal Fractures and Fixation Techniques
- Medical Imaging and Analysis
- HIV Research and Treatment
- Orthopaedic implants and arthroplasty
- Scoliosis diagnosis and treatment
- Bone Tissue Engineering Materials
- Monoclonal and Polyclonal Antibodies Research
- Bone and Dental Protein Studies
- Connective tissue disorders research
- Plant Toxicity and Pharmacological Properties
- Bone health and treatments
- Spinal Cord Injury Research
- Shoulder Injury and Treatment
- Tissue Engineering and Regenerative Medicine
- HIV/AIDS drug development and treatment
- Bone and Joint Diseases
- Glycosylation and Glycoproteins Research
- Nerve Injury and Rehabilitation
- Healthcare Systems and Public Health
- Stress Responses and Cortisol
Shanghai Jiao Tong University
2017-2024
Southeast University
2016-2024
Shanghai Sixth People's Hospital
2024
Wuhan University of Science and Technology
2024
Shanghai Ninth People's Hospital
2017-2023
Osaka University
2021
University of California, Los Angeles
2012-2017
Second Military Medical University
2008-2016
Shanghai Changzheng Hospital
2014-2015
Tsinghua University
1999-2012
Discography often destroys the hypoxic environment in intervertebral disc and accelerates degeneration (IVDD). Therefore, it fails to meet requirements for application clinical practice. This technology mainly increases reactive oxygen species (ROS) IVD. As so, is particularly critical develop strategies avoid this mechanism. Prussian blue nanoparticles (PBNPs) are found enhance development under magnetic resonance T1 have antioxidant enzyme activity. The key results of present study confirm...
As intervertebral disc degeneration (IVDD) proceeds, the dysfunctional mitochondria disrupt viability of nucleus pulposus cells, initiating degradation extracellular matrix. To date, there is a lack effective therapies targeting cells. Here, we synthesized polygallic acid-manganese (PGA-Mn) nanoparticles via self-assembly polymerization gallic acid in an aqueous medium and introduced mitochondrial peptide (TP04) onto using Schiff base linkage, resulting PGA-Mn-TP04 nanoparticles. With size...
Hypoxia is a characteristic of solid tumors that can lead to tumor angiogenesis and early metastasis, addressing hypoxia presents tremendous challenges. In this work, nanomedicine based on oxygen-absorbing perfluorotributylamine (PFA) the bioreductive prodrug tirapazamine (TPZ) was prepared by using polydopamine (PDA)-coated UiO-66 metal organic framework (MOF) as drug carrier.The results showed TPZ/PFA@UiO-66@PDA nanoparticles significantly enhanced hypoxia, induced cell apoptosis in vitro...
Retrospective analysis of kinematic magnetic resonance images.To provide baseline data on the segmental angular and translational motion degenerated cervical spine by subtype kyphotic deformity to elucidate relationship between degree spinal cord compression.Kyphotic deformities are relatively common classified as either global or focal. Nevertheless, effects compression unknown.A total 1171 symptomatic patients (618 females, 553 males) underwent imaging in neutral, flexion, extension...
ABSTRACT Osteogenic factors are often used in orthopedics to promote bone growth, improve fracture healing, and induce spine fusion. oxysterols naturally occurring molecules that were shown osteogenic differentiation vitro fusion vivo. The purpose of this study was identify an oxysterol more suitable for clinical development than those previously reported, evaluate its ability osteogenesis rats Among 100 analogues synthesized, Oxy133 induced significant expression markers Runx2, osterix...
Mechanical stimulation and WNT signalling have essential roles in regulating the osteogenic differentiation of bone marrow stromal cells (BMSCs) formation. However, little is known regarding regulation molecule expression therefore BMSCs during osteogenesis.Microarrays from elderly individuals or patients with osteoporosis (GSE35959) GEO database were analysed using GeneSight-Lite 4.1.6 (BioDiscovery) C2 curated gene sets downloaded Molecular Signatures Database (MSigDB). Realtime PCR...
Abstract Lumbar degenerative disc diseases cause low back pain (LBP). The maintenance of the height and stability intervertebral (IVD) space is an effective treatment for LBP. following study evaluated effects fibroblast injection on degeneration (IDD) in a preclinical setting. Compared with IDD group, group demonstrated IVD height, reduced endplate degeneration, improved nuclear magnetic resonance signals overall histological structure. In doing so, fibrotic IVDs maintained biomechanics...
Growth factors are of great potential in regenerative medicine. However, their clinical applications largely limited by the short vivo half-lives and narrow therapeutic window. Thus, a robust controlled release system remains an unmet medical need for growth-factor-based therapies. In this research, nanoscale (degradable protein nanocapsule) is established via situ polymerization on growth factor. The rate can be finely tuned engineering surface polymer composition. Improved outcomes...
The correlation between BMP-2 and osteosarcoma growth has gained increased interest in the recent years, however, there is still no consensus. In this study, we tested effects of on cells through both vitro vivo experiments. effect proliferation, migration invasion was vitro. Subcutaneous intratibial tumor models were used for experiments nude mice. EMT Wnt/β-catenin signaling pathway also using a variety biochemical methods. tests did not show significant cell proliferation. However,...
Background: Previous studies have suggested that the co-expression of two different bone morphogenetic protein (BMP) genes can result in production heterodimeric BMPs may be more potent than homodimers. In this study, combined BMP-2 and BMP-7 gene transfer was performed ex vivo to compare resulting new formation with single-BMP a rat spinal fusion model. Methods: Forty-four athymic rats underwent posterolateral at L4-L5 were implanted collagen sponge containing human adipose-derived stem...
OBJECTIVE The efficacy of some demineralized bone matrix (DBM) substances has been demonstrated in the spinal fusion rats; however, no previous comparative study reported DBM with human mesenchymal stem cells (hMSCs). There is an added cost to products cells, which should be justified by improved osteogenic potential. purpose this prospectively compare rates 3 different commercially available substances, both and without hMSCs. METHODS Posterolateral was performed 32 mature athymic nude...
Intervertebral disc degeneration (IDD) is an irreversible aging-associated clinical condition of unclear etiology. Mesenchymal stem cells (MSCs) have the potential to delay IDD, but mechanisms by which MSCs attenuate senescence-related nucleus pulposus (NPCs) remain uncertain. The present study employed a three-dimensional (3D) co-culture system explore influence on NPC induced TNF-α in rat cells. We found that with bone marrow-derived (BMSCs) reduced senescence-associated β-galactosidase...
Abstract Background Lower back pain is often accredited to loss of intervertebral disc (IVD) height and compromised spine stability as a result degeneration (IVDD). We aim locally use bleomycin induce the fibrotic transformation bone marrow stromal cells (BMSCs) means reparative fibrosis slow down loss. Methods IVDs from patients were gathered for histological examination. The expression transforming growth factor beta 1 (TGF-β) signaling pathway was determined by qPCR western blotting....
In vivo rodent model.Investigate the effect of systemic corticosteroid administration on soft-tissue inflammation after local delivery recombinant human bone morphogenetic protein-2 (rhBMP-2).Corticosteroid use in cases associated with rhBMP-2 has been reported clinical studies, but effectiveness its and appropriate timing remain unclear.Absorbable collagen sponges were implanted control or into lumbar region rats subcutaneously intramuscularly. Four groups studied: group I, sponge only; 2,...
Secreted phosphoprotein 24 kD (spp24) has been shown to inhibit bone morphogenetic protein 2 (BMP2)-induced cancer growth in several tumor models. In this study, we aimed investigate the effects spp24 on of prostate caused by BMP2 vitro and vivo.The PC-3 cell viability were analyzed using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay. A subcutaneous model intratibial was established cells. Tumor assessed through gross examination radiography during experiment....
Low back pain (LBP) is a very common condition and leads to serious pain, disability, price tag all over the world. Intervertebral disk degeneration (IDD) one of major reasons that contributed LBP. The levels interleukin 1 beta (IL-1β) increase significantly in degenerative disks. IL-1β also accelerates IDD. Sinapic acid (SA) has effect anti-inflammatory, antioxidant antimicrobial. However, SA on IDD never been studied. Therefore, aim this study was figure out whether protective nucleus...