A5040935744- Fatty Acid Research and Health
- Lipid metabolism and biosynthesis
- Cell death mechanisms and regulation
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Mitochondrial Function and Pathology
- Animal Virus Infections Studies
- Animal Disease Management and Epidemiology
- RNA regulation and disease
- Adipokines, Inflammation, and Metabolic Diseases
- Metabolism and Genetic Disorders
- Microbial infections and disease research
- Antimicrobial Peptides and Activities
- Cholesterol and Lipid Metabolism
- Bacterial biofilms and quorum sensing
- Autophagy in Disease and Therapy
- Redox biology and oxidative stress
- Animal health and immunology
- Microbial Metabolism and Applications
- Immune Cell Function and Interaction
- Peroxisome Proliferator-Activated Receptors
- Cancer, Lipids, and Metabolism
- RNA Interference and Gene Delivery
- NF-κB Signaling Pathways
- Brucella: diagnosis, epidemiology, treatment
- Pancreatic function and diabetes
Hospital Italiano de Buenos Aires
2021
Marqués de Valdecilla University Hospital
2019
Universidad de Zaragoza
2001-2016
Instituto de Investigación Sanitaria Aragón
2016
Cancer Research UK Scotland Institute
1992
Estación Experimental de Aula Dei
1992
Consejo Superior de Investigaciones Científicas
1992
Western Infirmary
1992
University of the Free State
1979
Abstract Preformed Fas ligand (FasL) and APO2 (APO2L)/TNF-related apoptosis-inducing (TRAIL) are stored in the cytoplasm of human Jurkat T cell line normal blasts. The rapid release these molecules their bioactive form is involved activation-induced death. In this study, we show by confocal microscopy that FasL APO2L/TRAIL mainly localized lysosomal-like compartments cells. We also immunoelectron inside cytoplasmic ∼500 nm diameter, with characteristics multivesicular bodies. Most share...
// Alberto Bosque 1, 4, * , Lisa Dietz 2, Ana Gallego-Lleyda Manuel Sanclemente 1 María Iturralde Javier Naval Angeles Alava Luis Martínez-Lostao 3, 5, # Hermann-Josef Thierse Anel Departamento de Bioquímica y Biología Molecular Celular, Facultad Ciencias, Universidad Zaragoza, Instituto Investigación Sanitaria Aragón (IIS-Aragón), Spain 2 Research Group for Immunology & Proteomics, Department of Dermatology, University Medical Center Mannheim, Ruprechts-Karls-University, Heidelberg, Germany...
ABSTRACT We have isolated from calf serum a protein with an apparent M r of 120,000. The was detected by using antibodies against major acute-phase in pigs acute inflammation. amino acid sequence internal fragment revealed that this is the bovine counterpart ITIH4, heavy chain 4 inter-alpha-trypsin inhibitor family. response sera determined for animals during experimental bacterial and viral infections. In model, were inoculated mixture Actinomyces pyogenes , Fusobacterium necrophorum...
IMS (intermembrane space) SOD1 (Cu/Zn-superoxide dismutase) is inactive in isolated intact rat liver mitochondria and activated following oxidative modification of its critical thiol groups. The present study aimed to identify biochemical pathways implicated the regulation activity assess impact functional state on key mitochondrial events. Exogenous H2O2 (5 μM) mitochondria. However, neither alone nor presence peroxiredoxin III SOD1, which was purified from subsequently reduced by...
The acute‐phase expression of pig MAP (major protein)/ITIH4 (inter‐α‐trypsin inhibitor heavy chain 4) and haptoglobin were analysed in primary cultures isolated hepatocytes response to recombinant human (rh) cytokines: tumor necrosis factor‐α (TNF‐α), interleukin‐1 (IL‐1), interleukin‐6 (IL‐6), as well bacterial lipopolysaccharide (LPS). Analysis MAP/ITIH4 mRNAs was carried out by RT‐PCR amplification. Secreted proteins from the cytokine‐treated quantified immunochemical techniques....
In this work, apolipoprotein A-I (ApoA-I) was purified from pig sera. The responses of protein after sterile inflammation and in animals infected with Actinobacillus pleuropneumoniae or Streptococcus suis were investigated. Decreases the concentrations ApoA-I, two to five times lower than initial values, observed at 2 4 days. It is concluded that ApoA-I a negative acute-phase pigs.
The localization of Cu,Zn-superoxide dismutase in the mitochondrial intermembrane space suggests a functional relationship with superoxide anion (O2*-) released into this compartment. present study was aimed at examining functionality and elucidating molecular basis for its activation space. Intact rat liver mitochondria neither scavenged nor dismutated externally generated O2*-, unless outer membrane disrupted selectively by digitonin. following disruption largely inhibited bacitracin, an...
Abstract A systematic study was undertaken to characterize the role of APO 2 ligand/tumor necrosis factor-related apoptosis-inducing ligand (APO2L/TRAIL) and Fas (FasL) together with expression several anti- or proapoptotic proteins in down-regulation normal human T cell responses. We have observed for first time that higher sensitivity blasts apoptosis activation-induced death (AICD) as compared naïve cells correlates increased Bcl-x short (Bcl-xS) Bim. die absence interleukin (IL-2) no...
Background. Recently, novel atypical Brucella strains isolated from humans and wild rodents have been reported. They are phenotypically close to Ochrobactrum species but belong the genus Brucella, based on genetic relatedness, although diversity is higher among than between classic species. were classified within or inopinata. However, with exception of microti, virulence these has not investigated in experimental models infection. Methods. The type B. inopinata strain BO1 (isolated a human)...
The interaction of fatty acids with rat α‐fetoprotein and albumin was measured using a partition equilibrium method. α‐Fetoprotein (AFP) displays one high‐affinity binding site for near two sites. AFP association constants most were similar to those (in the 10 7 M −1 range) whereas docosa‐hexaenoic acid it 9.7 × 8 , about 50‐fold higher than that corresponding albumin. This difference justifies docosahexaenoic in fetal or neonatal serum being mainly bound can indicate highly specific role...
To detect an early increase in the inflammatory response might prove to be vital for mitigating deleterious effects of disease over time.A 52-year-old obese man with moderate asthma and hypertension, who developed COVID-19 had symptoms, used a wearable device record heart rate variability (HRV) during his illness. He low parasympathetic tone, which decreased daily until it reached almost 2 standard deviations (SD) below normal values at end second week. His sympathetic tone increased from >...