A5045955472- 3D Printing in Biomedical Research
- Micro and Nano Robotics
- Neonatal Respiratory Health Research
- Cystic Fibrosis Research Advances
- Inhalation and Respiratory Drug Delivery
- Tracheal and airway disorders
- Marine Invertebrate Physiology and Ecology
- Pluripotent Stem Cells Research
- Genetic and Kidney Cyst Diseases
- Biomimetic flight and propulsion mechanisms
- Lattice Boltzmann Simulation Studies
- Cellular Mechanics and Interactions
- Tuberous Sclerosis Complex Research
- Liver Disease Diagnosis and Treatment
- Protist diversity and phylogeny
- Cephalopods and Marine Biology
- Liver physiology and pathology
- Asthma and respiratory diseases
- Neuroscience and Neural Engineering
- Tissue Engineering and Regenerative Medicine
- Oceanographic and Atmospheric Processes
- Innovative Microfluidic and Catalytic Techniques Innovation
- Alcohol Consumption and Health Effects
- Aquatic Ecosystems and Phytoplankton Dynamics
- Respiratory Support and Mechanisms
Helmholtz Zentrum München
2022-2025
Institute of Biological and Medical Imaging
2023-2025
University of Southern California
2020-2025
Hastings Center
2020-2025
Institute of Cell Biology and Neurobiology
2024
Southern California University for Professional Studies
2024
Gene Therapy Laboratory
2024
Pioneer (United States)
2023-2024
Leibniz Association
2024
University of Iowa
2024
A rat, dog, and human Liver-Chip designed using microengineered Organs-on-Chips technology recapitulates species-specific drug toxicities.
We show that mucociliary membranes of animal epithelia can create fluid-mechanical microenvironments for the active recruitment specific microbiome host. In terrestrial vertebrates, these tissues are typically colonized by complex consortia and inaccessible to observation. Such be directly examined in aquatic animals, providing valuable opportunities analysis activity relation bacteria recruitment. Using squid-vibrio model system, we provide a characterization initial engagement microbial...
Viral-induced exacerbation of asthma remains a major cause hospitalization and mortality. New human-relevant models the airways are urgently needed to understand how respiratory infections may trigger attacks advance treatment development. Here, we describe new model rhinovirus-induced that recapitulates viral infection asthmatic airway epithelium neutrophil transepithelial migration, enables evaluation immunomodulatory therapy. Specifically, microengineered fully differentiated human...
Mucociliary clearance is a vital defense mechanism of the human airways, protecting against harmful particles and infections. When this process fails, it contributes to respiratory diseases like chronic obstructive pulmonary disease (COPD) asthma. While advances in single-cell transcriptomics have revealed complexity airway composition, much what we know about how structure impacts relies on animal studies. This limits our ability create accurate human-based models diseases. Here show that...
Pharmaceutical screening based on human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) and multi electrode arrays (MEAs) have been proposed as a complementary method for electrophysiological safety efficacy assessment in drug discovery development. Contrary to animal models, these cells offer genetic background but, at present, fail recapitulate the mechanical structural properties of native myocardium. Here, we report that topographical cues soft micromolded gelatin can...
Human lung function is intricately linked to blood flow and breathing cycles, but it remains unknown how these dynamic cues shape human airway epithelial biology. Here we report a state-of-the-art protocol for studying the effects of medium airflow as well stretch on primary cell differentiation maturation, including mucociliary clearance, using an organ-on-chip device. Perfused cultures displayed accelerated maturation polarization changes in specific cell-types when compared traditional...
Microelectrode array recordings of neuronal activity present significant opportunities for studying the brain with single-cell and spike-time precision. However, challenges in device manufacturing constrain dense multisite to two spatial dimensions, whereas access three-dimensional (3D) structure many regions appears remain a challenge. To overcome this limitation, we novel recording modalities silicon-based devices aimed at establishing 3D functionality. First, fabricated dual-side...
Abstract We use a three-dimensional computational model to study the fluid transport and mixing due beating of an infinite array cilia. In accord with recent experiments, we observe two distinct regions: region above cilia below tip. The metachronal wave phase differences between neighbouring is known enhance ciliary this work, show that also enhances rates in sub-ciliary region, often simultaneously flow rate enhancement. Our results suggest simultaneous enhancement shear flow. As...
Organ-on-chip platforms aim to improve preclinical models for organ-level responses novel drug compounds. Heart-on-a-chip assays in particular require tissue engineering techniques that rely on labor-intensive photolithographic fabrication or resolution-limited 3D printing of micropatterned substrates, which limits turnover and flexibility prototyping. We present a rapid automated method large scale on-demand micropatterning gelatin hydrogels organ-on-chip applications using biocompatible...
We examine the hydrodynamic performance of two cilia beating patterns reconstructed from experimental data. In their respective natural systems, correspond to: (A) pumping-specialized cilia, and (B) swimming-specialized cilia. compare these as a function metachronal coordination in context model systems: swimming ciliated cylinder fluid pumping by carpet. Three measures are used for this comparison: (i) average speed/pumping flow rate; (ii) maximum internal moments generated cilia; (iii)...
Mucus plays an integral role for the barrier function of many epithelial tissues. In human airways, mucus is constantly secreted to capture inhaled microbes and pollutants cleared away through concerted ciliary motion. Many important respiratory diseases exhibit altered flowability impaired clearance, contributing distress increased risk infections. Understanding how rheology changes during disease progression in response treatments thus great interest subtyping patients tailoring...
Lymphangioleiomyomatosis (LAM) is a progressive lung disease with limited treatments, largely due to an incomplete understanding of its pathogenesis. Lymphatic endothelial cells (LECs) invade LAM cell clusters, which include HMB-45-positive epithelioid and smooth muscle α-actin-expressing LAM-associated fibroblasts (LAMFs). Recent evidence shows that LAMFs resemble cancer-associated fibroblasts, LAMF-LEC interactions contributing progression. To explore these mechanisms, we used spatial...
Energy demands are becoming recognized as an important constraint on neural signaling. The olfactory glomerulus provides a well defined system for analyzing this question. Odor stimulation elicits high-energy in glomeruli where axons converge onto dendrites of bulb neurons. We performed quantitative analysis the energy each type neuronal element within glomerulus. This included volumes element, their surface areas, and ion loads associated with membrane potentials synaptic activation...
Locomotion and feeding in marine animals are intimately linked to the flow dynamics created by specialized body parts. This interaction is of particular importance during ontogeny, when changes behaviour scale challenge organism with shifts fluid regimes altered functionality. Previous studies have indicated that Scyphozoan jellyfish ontogeny accommodates associated increasing dimensions velocities development. However, addition that-to a certain degree-underlie control animal, also...
Primary ciliary dyskinesia (PCD) is a rare heterogenic genetic disorder associated with perturbed biogenesis or function of motile cilia. Motile cilia dysfunction results in diminished mucociliary clearance (MCC) pathogens the respiratory tract and chronic airway inflammation infections successively causing progressive lung damage. Current approaches to treat PCD are symptomatic, only, indicating an urgent need for curative therapeutic options. Here, we developed vitro model based on human...