A5041012778- Animal Virus Infections Studies
- Virus-based gene therapy research
- Viral gastroenteritis research and epidemiology
- Microbial infections and disease research
- Parvovirus B19 Infection Studies
- Antibiotic Resistance in Bacteria
- Viral Infections and Immunology Research
- Streptococcal Infections and Treatments
- Rabies epidemiology and control
- Pharmaceutical and Antibiotic Environmental Impacts
- Aquaculture disease management and microbiota
- Antibiotics Pharmacokinetics and Efficacy
- Herpesvirus Infections and Treatments
- Viral Infections and Vectors
- Vector-borne infectious diseases
- Antibiotic Use and Resistance
- Bacteriophages and microbial interactions
- Vector-Borne Animal Diseases
- SARS-CoV-2 and COVID-19 Research
- Salmonella and Campylobacter epidemiology
- Antimicrobial Peptides and Activities
- Herbal Medicine Research Studies
- Fungal Biology and Applications
- Animal Disease Management and Epidemiology
- Veterinary medicine and infectious diseases
National Pingtung University of Science and Technology
2016-2025
National University of Singapore
2014
Chang Gung University
2012
Chang Gung Memorial Hospital
2012
National Taiwan University
2008-2011
Since late 2013, several outbreaks of porcine epidemic diarrhea virus (PEDV) infection have emerged in Taiwan. Suckling piglets under 2 weeks age showed severe vomiting and watery yellowish with morbidity mortality ranging from 80 to 100% 90 100%, respectively. A total 68 samples 25 pig farms were confirmed as positive for PEDV negative rotavirus transmissible gastroenteritis by reverse transcription PCR, the partial S gene was analyzed. Phylogenetic analysis places all 18 Taiwanese isolates...
Significance Dengue virus (DENV) infects almost 400 million people annually and some of these infections result in life threatening disease. An incomplete understanding pathogenesis, particularly on how non- or subneutralizing levels antibody augments DENV infection cells expressing Fc-gamma receptors (FcγRs), has hampered vaccine development. Here, we show that, to overcome the activating FcγR-dependent expression type-I interferon stimulated genes (ISGs), binds activates inhibitory...
Background/Objectives: Porcine reproductive and respiratory syndrome virus (PRRSV) has a major impact on swine productivity. Modified-live vaccines (MLVs) are used to aid in control. We investigated the cross-protection provided by lineage 7 PRRSV MLV against 1 isolate under laboratory conditions 3 challenge field Taiwan. Methods: In first study, thirty PRRS antibody-negative conventional piglets were vaccinated via intramuscular (IM) or intradermal (ID) route, with control group receiving...
Taiwan has been considered free from canine parvovirus type 2c (CPV-2c) based on the last report of 2 (CPV-2) surveillance. However, since January 2015, first CPV-2c in a puppy occurred Taiwan. There is currently limited information about variant In present study, we characterized previously unidentified and investigated distribution CPV-2 variants During 2014 to April 2016, fecal or rectal swab samples 99 dogs with suspected infection were collected. Eighty-eight identified as being either...
Fifty-one cats histopathologically confirmed to have been naturally infected by feline infectious peritonitis (FIP), were collected analyse the clinical and laboratory findings characterise disease staging. Effusive FIP was found in 33 cats, non-effusive 12 mixed-type six cats. Highly significant decreases haematocrit albumin levels an increase total bilirubin level noted both effusive FIP, at first presentation before death. In serial blood examinations of group, anaemia increases aspartate...
Diarrhea is one of the most common clinical symptoms reported in companion animal clinics. Dog circovirus (DogCV) a new mammalian that considered to be cause alimentary syndromes such as diarrhea, vomiting and hemorrhagic enteritis. DogCV has previously only been identified United States, Italy, Germany (GeneBank accession number: KF887949) China KT946839). Therefore, aims this study were determine prevalence Taiwan explore correlation between diarrhea infection. Clinical specimens collected...
Canine parvovirus type 2 (CPV-2) was first identified in the late 1970s; it causes intestinal hemorrhage with severe bloody diarrhea kennels and dog shelters worldwide. Since its emergence, CPV-2 has been replaced new genetic variants (CPV-2a, CPV-2b, CPV-2c). Currently, information about genotype prevalence of Vietnam is limited. In present study, we investigated distribution three regions Vietnam. Rectal swabs were collected from 260 dogs suspected infection northern, central, southern...
Abstract Background Glässer’s disease, caused by Glaesserella parasuis ( G. ), is a widespread bacterial infection in swine that leads to significant economic losses. , member of the normal microbiota within Pasteurellaceae family, exhibits horizontal resistance gene exchange and intracellular invasion capabilities, increasing risk developing resistant isolates. Accurate antimicrobial therapy essential for controlling disease. The production systems exotic crossbred pigs Taiwan black differ...
Background/Objectives: Porcine circovirus type 2 (PCV2) has a major impact on swine productivity. Vaccines are used to aid in control and mitigate production losses. We investigated the protection provided by an intradermal PCV2 vaccine against field strain Taiwan. Methods: conducted safety efficacy study. In study, four Specific Pathogen Free (SPF) piglets were enrolled One was selected as left unvaccinated, one be intradermally vaccinated with five times standard dose (1 mL, Porcilis® PCV...
Porcine circovirus type 2 (PCV2) and Mycoplasma hyopneumoniae (MHP) are both important common pathogens in the pig industry. Both major contributors to porcine respiratory disease complex serve potentiate other bacterial infections such as Actinobacillus pleuropneumonia. This study aims evaluate efficacy of a ready-to-use bivalent PCV2 MHP vaccine field under naturally PCV2-infected farms against existing monovalent options. We evaluated viremia, antibodies, lung lesion scores slaughtered...
Canine parvovirus 2 (CPV 2) is a major infectious cause of mortality in puppies. The characteristic symptom CPV disease intestinal hemorrhage with severe bloody diarrhea. Soon after was first recognized the late 1970s, original virus, 2, replaced canine population by strains carrying minor antigenic variants (termed 2a, 2b, and 2c) VP2 gene that could be distinguished using monoclonal antibodies molecular analyses. Here, we provide an updated characterization circulating Taiwan.In this...
This study aimed to investigate the in vitro susceptibilities of carbapenem-non-susceptible Pseudomonas aeruginosa (CNSPA) and Acinetobacter baumannii (CNSAB) isolates cefiderocol, novel β-lactamase inhibitor (BLI) combinations, new tetracycline analogues, other comparative antibiotics.In total, 405 non-duplicate bacteremic CNSPA (n = 150) CNSAB 255) were collected from 16 hospitals Taiwan between 2018 2020. Minimum inhibitory concentrations (MICs) determined using broth microdilution...
Two variants of porcine reproductive and respiratory syndrome virus (PRRSV), PRRSV 1 2, have caused abortion in pregnant sows distress nursery pigs worldwide. 2 has been thoroughly researched Taiwan since 1993; however, the first case was not reported until late 2018. To decipher genetic characteristics Taiwan, open reading frame 5 (ORF5) genes strains collected from 11 individual pig farms 2019-2020 were successfully sequenced. All Taiwanese ORF5 sequences closely related to Spanish-like...
Synthesis of the negative-strand ((-)-strand) counterpart is first step coronavirus (CoV) replication; however, detailed mechanism early event and factors involved remain to be determined. Here, using bovine (BCoV)-defective interfering (DI) RNA, we showed that (a) a poly(A) tail with length 15 nucleotides (nt) was sufficient initiate efficient (-)-strand RNA synthesis (b) substitution poly(U), (C) or (G) only slightly decreased efficiency synthesis. The findings indicate in addition tail,...
Abstract Porcine circovirus type 2 (PCV2) is an important pathogen that causes significant economic losses in the swine industry worldwide. Five major PCV2 genotypes have been identified, including PCV2a, PCV2b, PCV2c, PCV2d, and PCV2e. To investigate prevalence phylodynamics of different Taiwan, 214 ORF2 sequences from Taiwan other countries were analyzed. Genotypic differences observed among 2b, 2d at amino acid position 89 ORF2, with isoleucine (I), arginine (R), leucine (L),...
// Chieh-Yu Pan 1 , Chao-Nan Lin 2 Ming-Tang Chiou Chao Yuan Yu 3 Jyh-Yih Chen 4 and Chi-Hsien Chien Department Graduate Institute of Aquaculture, National Kaohsiung Marine University, Nanzih Dist., Kaohsiung, Taiwan Veterinary Medicine, College Pingtung University Science Technology, Neipu, Pingtung, Genomics BioSci & Tech Co., Ltd., Xizhi New Taipei, Research Station, Cellular Organismic Biology, Academia Sinica, Jiaushi, Ilan, Correspondence: Chien, email: Chen, Keywords : antimicrobial...
New variants of porcine epidemic diarrhoea virus (PEDV) causing a highly contagious intestinal disease, (PED), have resulted in high mortality suckling pigs across several countries since 2013. After 2015, the prevalence genogroup 2b (G2b) PEDVs decreased cyclical pattern with endemic seasonal outbreaks occasionally seen. To better understand genetic diversity recently circulating Taiwan, full-length spike (S) genes 31 PEDV strains from 28 pig farms collected during 2016-2018 were sequenced....
Porcine reproductive and respiratory syndrome (PRRS) causes significant economic losses in the swine industry worldwide. The PRRS virus (PRRSV) can be divided into two species, PRRSV 1 (European) 2 (North American). In Taiwan, isolates are dominant cause symptoms nursing pigs. From October to November 2018, a pig herd central pregnant sows had abortions stillbirths, piglets suffered from disorders. Laboratory tests identified presence of serum suckling this scenario. complete genome strain...
A multicenter collection of bacteremic isolates Escherichia coli (n = 423), Klebsiella pneumoniae 372), Pseudomonas aeruginosa 300), and Acinetobacter baumannii complex 199) was analyzed for susceptibility. Xpert Carba-R assay sequencing mcr genes were performed carbapenem- or colistin-resistant isolates. Nineteen (67.8%) carbapenem-resistant K. 28) one (20%) E. 5) isolate harbored blaKPC 17), blaOXA-48 2), blaVIM 1) genes.