A5046507576- Prion Diseases and Protein Misfolding
- Knee injuries and reconstruction techniques
- Periodontal Regeneration and Treatments
- Osteoarthritis Treatment and Mechanisms
- Bone Tissue Engineering Materials
- Neurological diseases and metabolism
- Lipoproteins and Cardiovascular Health
- Spaceflight effects on biology
- Bone health and osteoporosis research
- Trace Elements in Health
- Mesenchymal stem cell research
- Cancer, Hypoxia, and Metabolism
- Dental Implant Techniques and Outcomes
- Genetics, Aging, and Longevity in Model Organisms
- Angiogenesis and VEGF in Cancer
- Cholesterol and Lipid Metabolism
- Corneal surgery and disorders
- Parathyroid Disorders and Treatments
- Alzheimer's disease research and treatments
- Viral Infections and Vectors
- Medical Imaging and Pathology Studies
- Hormonal Regulation and Hypertension
- Neuroscience and Neuropharmacology Research
- Bone Metabolism and Diseases
- Heterotopic Ossification and Related Conditions
Istituto Clinico Sant'Ambrogio
2024
Istituto Ortopedico Galeazzi
2020-2022
Istituti di Ricovero e Cura a Carattere Scientifico
2022
University of Milan
1997-2019
Ospedale Policlinico San Martino
2012-2019
University of Genoa
2012-2019
Centro Cardiologico Monzino
2017
Alleanza Contro il Cancro
2011-2012
Northwestern University
2004-2012
Vrije Universiteit Amsterdam
2007
<b><i>Article abstract</i></b> Phenotypic heterogeneity of sporadic Creutzfeldt-Jakob disease (CJD) has been linked to biochemically distinct types the protease-resistant form prion protein (type 1 and type 2 PrP<sup>Sc</sup>). We investigated 14 cases CJD found that both PrP<sup>Sc</sup> coexisted in 5 subjects. The isoforms were associated with different patterns PrP deposition severity spongiform changes, suggesting plays a central role determining neuropathologic profile CJD.
Prion diseases are transmissible neurodegenerative conditions characterized by the accumulation of protease-resistant forms prion protein (PrP), termed PrPres, in brain. Insoluble PrPres tends to aggregate into amyloid fibrils. The anthracycline 4′-iodo-4′-deoxy-doxorubicin (IDX) binds fibrils and induces resorption patients with systemic amyloidosis. To test IDX an experimental model disease, Syrian hamsters were inoculated intracerebrally either scrapie-infected brain homogenate or...
Bone is a complex dynamic tissue undergoing continuous remodeling process. Gravity physical force playing role in the and contributing to maintenance of bone integrity. This article reports an investigation on alterations microarchitecture that occurred wild type (Wt) pleiotrophin-transgenic (PTN-Tg) mice exposed near-zero gravity International Space Station (ISS) during Mice Drawer System (MDS) mission, date, longest permanence (91 days) space. The transgenic mouse strain over-expressing...
The periosteum plays a pivotal role during bone development and repair contributing to vascularization osteoprogenitor cells source. We propose periosteal substitute engineered using platelet-rich plasma (PRP) membrane incorporating autologous marrow-derived mesenchymal stem (PRP/BMSC gel membrane) be wrapped around an osteoconductive scaffold for regeneration of compromised defects. PRP/BMSC was optimized different compositions optimal release vascular endothelial growth factor (VEGF)...
Abstract Lipocalin‐2 (LCN2) is a protein largely expressed in many tissues, associated with different biological phenomena such as cellular differentiation, inflammation and cancer acting survival/apoptotic signal. We found that LCN2 was during osteoblast differentiation we generated transgenic (Tg) mice over‐expressing bone. Tg were smaller presented bone microarchitectural changes both endochondral intramembranous bones. In particular, bones displayed thinner layer of cortical decreased...
Sprouty (Spry) proteins are negative regulators of receptor tyrosine kinase signaling; however, their exact mechanism action remains incompletely understood. We identified phosphatidylinositol-specific phospholipase C (PLC)-γ as a partner the Spry1 and Spry2 proteins. Spry-PLCγ interaction was dependent on Src homology 2 domain PLCγ conserved N-terminal residue in Spry2. Overexpression associated with decreased phosphorylation activity measured by production inositol (1,4,5)-triphosphate...
Bone is an active tissue where bone mineralization and resorption occur simultaneously. In the case of fracture, there are numerous factors required to facilitate healing including precursor cells blood vessels. To evaluate interaction between marrow-derived mesenchymal stem (BMSC)—the able differentiate into bone-forming human umbilical vein endothelial (HUVEC)—a cell source widely used for study We performed direct coculture BMSC HUVEC in normoxia chemically induced hypoxia using...
Musculoskeletal impairments, especially cartilage and meniscus lesions, are some of the major contributors to disabilities. Thus, novel tissue engineering strategies being developed overcome these issues. In this study, aim was investigate biocompatibility, in vitro vivo, a thermosensitive, injectable chitosan-based hydrogel loaded with three different primary mesenchymal stromal cells. The cell types were human adipose-derived cells (hASCs), bone marrow stem (hBMSCs), neonatal porcine...
Demineralized bone matrix (DBM) is widely used for regeneration. Since DBM prepared in powder form its handling properties are not optimal and limit the clinical use of this material. Various synthetic biological carriers have been to enhance handling. In study we evaluated effect gamma irradiation on physical-chemical Pluronic morphogenetic proteins (BMPs) amount samples. vivo studies were carried out investigate regeneration a irradiated DBM-Pluronic F127 (DBM-PF127) composite implanted...
The ACVR1 gene encodes a type I receptor of bone morphogenetic proteins (BMPs). Activating mutations in are responsible for fibrodysplasia ossificans progressiva (FOP), rare disease characterized by congenital toe malformation and progressive heterotopic endochondral ossification leading to severe cumulative disability. Until now, no therapy has been available prevent soft-tissue swelling (flare-ups) that trigger the process. With aim finding new therapeutic strategy FOP, we developed...
The neuropathological diagnosis of Creutzfeldt‐Jakob disease relies on the immunohistochemical demonstration proteinase‐K resistant form prion protein (PrP res ) in brain tissue. antigenicity PrP is strongly reduced by formalin solution widely used to fix tissue, thus immunoreactivity inconsistently detectable formalin‐fixed A better immunostaining can be obtained using Carnoy's fixing solution, which composed ethanol, chloroform and acetic acid (6:3:1). easily extracted from Carnoy's‐fixed,...
One of the current hypotheses on dopamine in physiology motivation posits that this neurotransmitter regulates filtering and selection inputs to nucleus accumbens. The effects (100 microM) D1-receptor agonist SKF 38393 (20-50 GABAergic synaptic transmission between pairs principal cells rat accumbens were studied by using simultaneous dual patch-clamp recordings acutely prepared brain slices. Both compounds attenuated postsynaptic responses induced presynaptic firing effect was reversed...
The meniscus possesses low self-healing properties. A perfect regenerative technique for this tissue has not yet been developed. This work aims to evaluate the role of hypoxia in meniscal development vitro. Menisci from neonatal pigs (day 0) were harvested and cultured under two different atmospheric conditions: (1% O2) normoxia (21% up 14 days. Samples analysed at 0, 7 days by histochemical (Safranin-O staining), immunofluorescence RT-PCR (in both methods SOX-9, HIF-1α, collagen I II),...
Due to their osteoconductive and inductive properties, a variety of calcium phosphate (CaP) scaffolds are commonly used in orthopaedics as graft material heal bone defects. In this study, we have two CaP with different hydroxyapatite (HA) β-tricalcium (β-TCP) ratios (MBCP®; 60/40 MBCP+®; 20/80) investigate intrinsic capacity favour human marrow stem cells (hBMSCs) osteogenic differentiation capacity. We report that MBCP+® showed vitro culture model higher rate ion release comparison MBCP®....
Sarcopenia, an age-related decline in muscle mass and strength, is associated with metabolic disease increased risk of cardiovascular morbidity mortality. It decreased tissue vascularization atrophy. In this work, we investigated the role hypoxia inducible factor HIF-1α sarcopenia. To end, obtained skeletal biopsies from elderly sarcopenic patients compared them those young individuals. We found a decrease expression its target genes sarcopenia, as well PAX7, major stem cell marker satellite...
The success of cell-based approaches for the treatment cartilage or fibro-cartilaginous tissue defects requires an optimal cell source with chondrogenic differentiation ability that maintains its differentiated properties and stability following implantation. For this purpose, aim study was to evaluate use endostatin (COL18A1), anti-angiogenic factor, which is physiologically involved in during meniscus development. Swine neonatal meniscal cells not yet subjected mechanical stimuli were...
Menisci play an essential role in shock absorption, joint stability, load resistance and its transmission thanks to their conformation. Adult menisci can be divided three zones based on the vascularization: avascular inner zone with no blood supply, a fully vascularized outer zone, intermediate zone. This organization, addition incomplete knowledge about meniscal biology, composition, gene expression, makes regeneration still one of major challenges both orthopedics tissue engineering. To...