A5074507485- RNA modifications and cancer
- Fungal and yeast genetics research
- Thyroid Disorders and Treatments
- Porphyrin and Phthalocyanine Chemistry
- Photodynamic Therapy Research Studies
- Laser Material Processing Techniques
- Neuroendocrine Tumor Research Advances
- Thyroid Cancer Diagnosis and Treatment
- Microbial Natural Products and Biosynthesis
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Nanoplatforms for cancer theranostics
- Cancer Cells and Metastasis
- Fungal Biology and Applications
- Ubiquitin and proteasome pathways
- Optical Coherence Tomography Applications
- Cancer Genomics and Diagnostics
- Ferroptosis and cancer prognosis
- Cancer-related molecular mechanisms research
- Advanced Fluorescence Microscopy Techniques
- Tribology and Lubrication Engineering
- Genetics and Neurodevelopmental Disorders
- Bacterial Genetics and Biotechnology
- Cell death mechanisms and regulation
- Laser Applications in Dentistry and Medicine
Gebze Technical University
2013-2024
Sağlık Bilimleri Üniversitesi
2024
University of Health Science
2024
Istanbul Medipol University
2024
Bilkent University
2006-2013
University of Naples Federico II
2010
Centre National de la Recherche Scientifique
1995-2003
Université Paris Cité
1994-2003
Albert Einstein College of Medicine
2000
Université Paris-Sud
1994-1997
We propose and demonstrate the use of short pulsed fiber lasers in surface texturing using MHz-repetition-rate, microjoule- sub-microjoule-energy pulses. Texturing titanium-based (Ti6Al4V) dental implant surfaces is achieved femtosecond, picosecond (for comparison) nanosecond pulses with aim controlling attachment human cells onto surface. Femtosecond yield similar results creation micron-scale textures greatly reduced or no thermal heat effects, whereas result strong effects. Various are...
In Aspergillus nidulans a highly specific l ‐proline transporter is encoded by the prnB gene which tightly linked to all other genes involved in proline catabolism. mycelia, expression of prn structural finely co‐regulated response induction and nitrogen/carbon catabolite repression. this study we establish that also activated during germination conidiospores. This activation persists until development 6 h‐old mycelia it independent mediated pathway‐specific prnA product. We then show that,...
The highly conserved RasGEF1 family of proteins contain a C‐terminal CDC25‐Ras exchange motif domain and an N‐terminal RasGEF‐N domain, are unknown function specificity. Using purified RasGEF1A RasGEF1B proteins, as well Ras we established that very specific factors for Rap2, member the Rap subfamily Ras‐like G‐proteins. They do not act on Rap1 or other members subfamily. Although Rap2 was implicated in regulation cell adhesion, establishment morphology, modulation synapses neurons, no...
Cytokinetic abscission is the cellular process leading to physical separation of two postmitotic sister cells by severing intercellular bridge. The most noticeable structural component bridge a transient organelle termed as midbody, localized at central region marking site abscission. Despite its major role in completion cytokinesis, our understanding spatiotemporal regulation midbody assembly limited. Here, we report first characterization coiled-coil domain-containing protein-124...
Abstract Background: In support of Project LUNGMARK, LUNGBANK was established as a pioneering biorepository dedicated to lung cancer. Designed for streamlined biospecimen handling, it employs high-volume technologies secure high-quality samples. Fortified with robust data management, efficiently integrates diverse datasets. This strategic infrastructure positions more than repository; operates sophisticated platform advanced cancer research, amplifying the potential meaningful discoveries....
The aim of this study was to comparatively investigate the effects 5-azacytidine-C (5-Aza), trichostatin-A (TSA), and all-trans retinoic acid (ATRA) on mRNA expressions Na/I symporter (NIS), thyroglobulin (Tg), thyroid peroxidase (TPO), stimulating hormone receptor (TSH-R), radioiodine (RAI) uptake in cancer (B-CPAP) normal (Nthy-ori 3-1) cell lines. Cell lines were treated with 10 ng/mL TSA, 5 μM 5-Aza, 1 ATRA, according MTT (methyl-thiazol-tetrazolium) test results. Additionally,...
Activity of the sodium/iodide symporter (NIS) in lactating breast is essential for iodide (I–) accumulation milk. Significant NIS upregulation was also reported cancer, indicating a potential use radioiodide treatment. All-trans-retinoic acid (tRA) potent ligand that enhances expression subset cancer cell lines and experimental models. Indirect tRA stimulation cells very well documented; however, direct by tRA-activated nuclear receptors has not been identified yet. Aiming to uncover...
In the filamentous fungus Aspergillus nidulans, L-proline uptake is mediated by product of prnB gene which codes for a member family amino acid transporters found both in pro- and eukaryotes. Regulation expression has previously been studied great detail at molecular level. However, no studies have addressed possible post-transcriptional controls or kinetic characterisation PrnB transporter. Here we develop rapid efficient method direct measurements proline germinating conidiospores A....
Background Breast cancer is a remarkably heterogeneous disease. Luminal, basal-like, "normal-like", and ERBB2+ subgroups were identified shown to have different prognoses. The mechanisms underlying this heterogeneity are poorly understood. In our study, we explored the role of cellular differentiation senescence as potential cause heterogeneity. Methodology/Principal Findings A panel breast cell lines, isogenic clones, tumors used. Based on their ability generate senescent progeny under...
Coiled-coil domain containing protein-124 (Ccdc124) is a putative mRNA-binding factor associated with cell division, and ribosome biology. Previous reports mentioned an up-regulation of CCDC124 gene in cancer, listed its mRNA molecular prognostic signature breast cancer.Establishing RNA-binding characteristics Ccdc124 for better functional characterization, carrying-out retrospective studies order to evaluate aberrant expression human cancer samples from various tissue origins.Bioinformatics...
The aim of this study was to comparatively investigate the effects 5-azacytidine-C (5-Aza), trichostatin-A (TSA), and all-trans retinoic acid (ATRA) on mRNA expressions sodium iodine (Na/I) symporter (NIS), thyroglobulin (Tg), thyroid peroxidase (TPO), thyroid-stimulating hormone receptor (TSH-R), as well radioiodine (RAI) uptake in cancer (B-CPAP) normal (Nthy-ori 3-1) cell lines. Cell lines were treated with 10 ng/mL TSA, 5 μM 5-AZA, 1 ATRA, according MTT (methyl-thiazol-tetrazolium) test...
Iodide (I-) is an essential constituent of the thyroid hormones triiodothyronine (T3) and thyroxine (T4), iodide concentrating mechanism gland for synthesis these hormones. In addition, differential uptake iodine isotopes (radioiodine) a key modality diagnosis therapy cancer. The sodium dependent transport activity mainly attributed to functional expression Na+/I- Symporter (NIS) localized at basolateral membrane thyrocytes. this paper, we review summarize current data on molecular...
Ataxia represents a heterogeneous group of neurodegenerative disorders characterized by loss balance and coordination, often resulting from mutations in genes vital for cerebellar function maintenance. Recent advances genomics have identified gene fusion events as critical contributors to various cancers diseases. However, their role ataxia pathogenesis remains largely unexplored. Our study Hdelved into this possibility analyzing RNA sequencing data 1443 diverse samples, including cell mouse...
CD81 (TAPA-1) is a ubiquitously expressed tetraspanin protein identified as component of the B lymphocyte receptor (BCR) and for Hepatitis C Virus. In an effort to identify trans-membrane proteins that interact with T-cell antigen (TCR), we performed membrane yeast two hybrid screen interactor CD3delta subunit TCR. We found in absence CD81, thymocytes from knockout mice, TCR engagement resulted stronger signals. These results were recapitulated T cell lines express low levels through shRNA...
PrnB, the l-proline transporter of Aspergillus nidulans, belongs to Amino acid Polyamine Organocation (APC) family conserved in prokaryotes and eukaryotes. In silico analysis limited biochemical evidence suggest that APC transporters comprise 12 transmembrane segments (TMS) connected with relatively short hydrophilic loops (L). However, very little is known on structure-function relationships transporters. This work makes use A. nidulans PrnB address by selecting, constructing analysing...
The administration of photosensitizers and their biocompatibility is crucial to the success photodynamic therapy treatment. Amongst various strategies developed administer low water-solubility, use polyvinylpyrrolidone (PVP), an FDA-approved food additive, emerging as extremely promising. Its effect on outcome was compared more classical formulation in DMSO direct solubilization water. tetrakis (2-(2-(2-methoxyethoxy)ethoxy)ethoxy)-Zn(II) phthalocyanine chosen model photosensitizer for this...
We report on the development of a ultrafast fiber laser-microscope system for femtosecond photodisruption biological targets. A mode-locked Yb-fiber laser oscillator generates few-nJ pulses at 32.7 MHz repetition rate, amplified up to ∼125 nJ 1030 nm. Following dechirping in grating compressor, ∼240 fs-long are delivered sample through diffraction-limited microscope, which allows real-time imaging and control. The can generate arbitrary pulse patterns, formed by two acousto-optic modulators...
Coiled-coil domain-containing protein-124 (Ccdc124) is a conserved eukaryotic ribosome-associated RNA-binding protein which involved in resuming ribosome activity after stress-related translational shutdown. Ccdc124 also detected at cellular localizations devoid of ribosomes, such as the centrosome, or cytokinetic midbody, but its translation-independent function currently unknown.
In an attempt to investigate its potential as a PDT [Formula: see text]F MRI molecular theranostic, Zn phthalocyanine with 24 pseudo-equivalent fluorine atoms was designed and prepared. Compared H analogues, the fluorinated derivative has much higher generation of singlet oxygen. NMR signals in CDCl 3 showed that all are magnetically only two close signals. Formulation PVP (polyvinylpyrrolidone), FDA-approved additive, enabled water-solubilization phthalocyanines but no satisfying signal...
Controlled modification of implant surfaces using femtosecond, picosecond and nanosecond pulses from home-built all-fiber-integrated lasers is demonstrated. Picosecond femtosecond offer superior control over the surface texture. Increasing cell attachment to discussed.
Abstract Objectives Gram-positive sporulated bacilli can cause many different diseases and isolation from environmental samples is difficult. Therefore, the quick detection diagnosis of these microorganisms have critical importance because their potentially harmful situation. However, accepted diagnostic methods exist, future technology points to immunoassay systems. Immunological detect biological require antigen-specific high-affinity antibodies as key materials. Methods In this study,...