A5069061241- Immune cells in cancer
- Neuroinflammation and Neurodegeneration Mechanisms
- Extracellular vesicles in disease
- Liver Disease Diagnosis and Treatment
- Liver Disease and Transplantation
- Cardiac Ischemia and Reperfusion
- Complement system in diseases
- Immune Cell Function and Interaction
- Phagocytosis and Immune Regulation
- Acute Ischemic Stroke Management
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Diabetes and associated disorders
- Parkinson's Disease Mechanisms and Treatments
- Pediatric Hepatobiliary Diseases and Treatments
- Multiple Myeloma Research and Treatments
- Hepatitis C virus research
- Liver Diseases and Immunity
- Anesthesia and Neurotoxicity Research
- Neonatal and fetal brain pathology
- Renal Diseases and Glomerulopathies
- Cytokine Signaling Pathways and Interactions
- Traumatic Brain Injury and Neurovascular Disturbances
- Vitamin D Research Studies
- Pancreatic function and diabetes
- Drug Transport and Resistance Mechanisms
Aarhus University Hospital
2016-2025
Aarhus University
2018
Summary Monocyte‐derived macrophages (MDMs) generated from peripheral blood monocytes are widely used to model human for in vitro studies. However, the possible impact of different isolation methods on resulting MDM phenotype is poorly described. We aimed investigate effects three commonly monocyte techniques phenotype. Plastic adhesion, negative selection, and CD14 pos selection were compared. by 5‐day culture with macrophage granulocyte–macrophage colony‐stimulating factors. investigated...
Abstract The hemoglobin receptor CD163 and the mannose CD206 are both expressed on surface of human macrophages. Upon inflammatory activation, receptors shed from macrophage generating soluble products. plasma concentration (sCD163) (sCD206) increased in several diseases, including conditions cancer. Here, we show that contrast to CD163, LPS-mediated shedding humans is slow a result indirect signaling. Although sCD163 sCD206 were response LPS stimulation vivo, only was LPS-stimulated...
Parkinson's disease (PD) is a neurodegenerative disorder with significant immune component, as demonstrated by changes in biomarkers patients' biofluids. However, which specific cells are responsible for those unclear because most can be produced various cell types.The aim of this study was to explore monocyte involvement PD.We investigated the monocyte-specific biomarker sCD163, soluble form receptor CD163, cerebrospinal fluid (CSF) and serum two experiments, compared it other clinical...
Background: Remote Ischemic Conditioning (RIC) is a simple and low-cost intervention that thought to increase collateral blood flow through the vasodilatory effects of nitric oxide (NO) produced by endothelium red cells (RBC). This study aims investigate whether RIC affects RBC deformability levels NO nitrite in ischemic stroke patients. Methods: predefined sub-study RESIST randomized clinical trial. was started ambulance continued at hospital for seven days. Patients with prehospital...
BACKGROUND: Remote ischemic conditioning (RIC) is a simple and low-cost intervention that thought to increase collateral blood flow through the vasodilatory effects of nitric oxide (NO) produced by endothelium red cells (RBCs). This study aims investigate whether RIC affects RBC deformability levels NO nitrite in patients with stroke. METHODS: predefined substudy RESIST (Remote Ischemic Conditioning Patients With Acute Stroke Trial) randomized clinical trial conducted Denmark. was started...
Abstract Objectives Extracellular vesicles (EVs) are important for intercellular signalling in cancer. Tumour‐associated macrophages, expressing the haemoglobin‐haptoglobin and mannose receptors CD163 CD206, crucial cancer progression. We recently identified on EVs circulation as a fraction of total soluble (sCD163). Here, we investigated presence CD206‐positive (EV‐CD163, EV‐CD206) patients with multiple myeloma (MM). Methods enrolled MM (n = 32), monoclonal gammopathy undetermined...
Macrophages synthesize active vitamin D (1,25-dihydroxy-vitamin D) and express the receptor in nucleus; however, metabolism relation to macrophage polarization function is not well understood. We studied monocyte-derived macrophages (MDMs) from human buffy coats polarized into M0, M1 (LPS + IFNγ), M2a (IL4 IL13) M2c (IL10) subtypes stimulated with 25-hydroxy-vitamin (1000 10,000 nanomolar). measured metabolites (25-hydroxy-vitamin D, 1,25-dihydroxy-vitamin 24,25-dihydroxy-vitamin...
Background and Aims: The macrophage “don’t eat me” pathway CD47/SIRPα is a target for promising new immunotherapy. We hypothesized that soluble variant of SIRPα present in the blood may function as biomarker. Methods: Monocyte derived macrophages (MDMs) from human buffy-coats were stimulated into subtypes by LPS IFN-γ (M1), IL-4 IL-13 (M2a), IL-10 (M2c) investigated using flow cytometry. Soluble (sSIRPα) was measured cell cultures serum Western blotting an optimized ELISA. Serum samples...
Background: Extracellular vesicles (EVs) are implicated in intercellular communication liver diseases. An EV-associated fraction of the macrophage biomarker soluble CD163, denoted EV-CD163, was recently identified. EV-CD163 may be released during later phases inflammatory response as opposed to acute shedding CD163 ectodomain (Ecto-CD163). Total sCD163 is a well-described inflammation, and we investigated distribution fractions along with CD206 (EV-CD206) patients chronic alcoholic...
The macrophage activation marker soluble (s)CD163 is associated with disease severity and prognosis in patients primary biliary cholangitis (PBC). Ursodeoxycholic acid (UDCA) treatment attenuates fibrosis progression PBC patients, but its effect on unclear. We examined the of UDCA activation, as determined by sCD163 levels.We included 2 cohorts patients; 1 cohort prevalent incident before start follow-up after 4 weeks 6 months. measured liver stiffness both cohorts. Further, we TNF-α...
Abstract Objectives Soluble (s) CD163 is a well-established macrophage biomarker, and recent data suggests urine sCD163 to reflect disease activity in crescentic glomerulonephritis (GN). Other types of GN may also be associated with glomerular inflammation but the potential usefulness as general biomarker remains unaddressed. Methods An in-house enzyme-linked immunosorbent assay (ELISA) was validated for urinary use compared frequently used commercial ELISA. The pre-analytical stability...