Photo-acoustic (PA) imaging has been developed for different purposes, but recently, the modality gained interest with applications to small animal imaging. As a technique it is sensitive endogenous optical contrast present in tissues and, contrary diffuse imaging, promises bring high resolution vivo studies at midrange depths (3-10 mm). Because of limited amount radiation can be exposed to, existing reconstruction algorithms circular tomography require great number measurements and...

Very high frame rate ultrasound imaging has recently allowed for the extension of applications echography to new fields study such as functional brain, cardiac electrophysiology, and quantitative intrinsic mechanical properties tumors, name a few, non-invasively in real time. In this study, we present first implementation Ultrafast Ultrasound Imaging 3D based on use either diverging or plane waves emanating from sparse virtual array located behind probe. It achieves contrast resolution while...

Ultrasound localization microscopy can map blood vessels with a resolution much smaller than the wavelength by localizing microbubbles. The current implementations of technique are limited to 2-D planes or small fields view in 3-D. These suffer from minutelong acquisitions, out-of-plane microbubbles, and tissue motion. In this paper, we exploit recent development 4D ultrafast ultrasound imaging insonify an isotropic volume up 20000 times per second perform three dimensions. Specifically, 32...

Ultrafast Doppler imaging was introduced as a technique to quantify blood flow in an entire 2-D field of view, expanding the application ultrasound highly sensitive anatomical and functional mapping vessels. We have recently developed 3-D ultrafast imaging, that can produce thousands volumes per second, based on plane diverging wave emissions, demonstrated its clinical feasibility human subjects vivo. In this study, we show noninvasive power Doppler, pulsed color be used perform vessels...

We present herein 4D ultrafast ultrasound flow imaging, a novel ultrasound-based volumetric imaging technique for the quantitative mapping of blood flow. Complete distribution was achieved through 2D tilted plane-wave insonification, multi-angle cross-beam beamforming, and 3D vector Doppler velocity components estimation by least-squares fitting. performed in large fields view at very high volume rate (>4000 volumes s-1) using 1024-channel scanner matrix-array transducer. The precision...

Over the last ten years, shear wave elastography (SWE) has seen considerable development and is now routinely used in clinics to provide mechanical characterization of tissues improve diagnosis. The most advanced technique relies on use an ultrafast scanner generate image waves real time a 2-D plane at several thousands frames per second. We have recently introduced 3-D ultrasound imaging acquire with matrix probes propagation generated by dedicated radiation pressure transducer single...

Ultrasound Localization Microscopy (ULM) can resolve the microvascular bed down to a few micrometers. To achieve such performance, microbubble contrast agents must perfuse entire network. Microbubbles are then located individually and tracked over time sample individual vessels, typically hundreds of thousands images. overcome fundamental limit diffraction dense reconstruction network, low concentrations be used, which leads acquisitions lasting several minutes. Conventional processing...

Abstract A rise in blood flow velocity variations (i.e. pulsatility) the brain, caused by stiffening of upstream arteries, is associated with cognitive impairment and neurodegenerative diseases. The study this phenomenon requires brain-wide pulsatility measurements, large penetration depth high spatiotemporal resolution. development dynamic ultrasound localization microscopy (DULM), based on ULM, has enabled measurements rodent brain 2D. However, 2D imaging accesses only one slice measures...

The hypoglycaemic effect of orally administered extracts fruits cultivated Momordica charantia (karela) was examined in normal and streptozotocin diabetic mice. In mice, an aqueous extract (A) lowered the glycaemic response to both oral intraperitoneal glucose, without altering insulin response. This residue after alkaline chloroform extraction (B) reduced hyperglycaemia mice at 1 hour. Material recovered by acid water wash remaining (D) produced a more slowly generated effect. results...

Three flavonoids which promoted Escherichia coli topoisomerase IV-dependent DNA cleavage were isolated from cottonseed flour and identified as quercetin 3-O-beta-D-glucose-[1,6]-O-alpha-L-rhamnose (rutin), 3-O-beta-D-galactose-[1,6]-O-alpha-L-rhamnose, 3-O-beta-D-glucose (isoquercitrin). The most active one (rutin) also inhibited decatenation activity (50% inhibitory concentration, 64 microg/ml) induced the SOS response of a permeable E. strain. Derivatives glycosylated at position C-3 shown...

High frame-rate ultrasound RF data acquisition has been proved to be critical for novel cardiovascular imaging techniques, such as high-precision myocardial elastography, pulse wave (PWI), and electromechanical (EWI). To overcome the limitations on standard clinical systems, we developed an automated method multi-sector through retrospective electrocardiogram (ECG) gating a clinically used open architecture system. The achieved both high spatial (64 beam density) temporal resolution (frame...

Electromechanical wave imaging (EWI) is a novel ultrasound-based modality for mapping of the electromechanical (EW), i.e. transient deformations occurring in immediate response to electrical activation. The correlation between EW and activation has been established prior studies. However, methods used previously map required reconstruction images over multiple cardiac cycles, precluding application EWI non-periodic arrhythmias such as fibrillation. In this study, new sequences are developed...

Cardiac conduction abnormalities remain a major cause of death and disability worldwide. However, as today, there is no standard clinical imaging modality that can noninvasively provide maps the electrical activation. In this paper, electromechanical wave (EWI), novel ultrasound-based method, shown to be capable mapping electromechanics all four cardiac chambers at high temporal spatial resolutions precision previously unobtainable in full view both animals humans. The transient deformations...

4D ultrafast ultrasound imaging was recently shown using a 2D matrix (i.e. fully populated) connected to 1024-channel scanner. In this study, we investigate the row-column addressing (RCA) approach, which allows reduction of independent channels from N × + N, with dedicated beamforming strategy for based on coherent compounding orthogonal plane wave (OPW). OPW is transmissions in one direction receive along and its companion sequence. Such recombination complementary sequences leads virtual...

The assessment of myocardial fiber disarray is major interest for the study progression disease. However, time-resolved imaging structure remains unavailable in clinical practice. In this study, we introduce 3D Backscatter Tensor Imaging (3D-BTI), an entirely novel ultrasound-based technique that can map fibers orientation and its dynamics with a temporal resolution 10 ms during single cardiac cycle, non-invasively vivo entire volumes. 3D-BTI based on ultrafast volumetric ultrasound...

Transthoracic shear-wave elastography (SWE) of the myocardium remains very challenging due to poor quality transthoracic ultrafast imaging and presence clutter noise, jitter, phase aberration, ultrasound reverberation. Several approaches, such as diverging-wave coherent compounding or focused harmonic imaging, have been proposed improve quality. In this study, we introduce (UHCC), in which pulse-inverted diverging waves are emitted coherently compounded, show that an approach can be used...

Electromechanical wave imaging (EWI) has recently been introduced as a noninvasive, ultrasound-based modality, which could map the electrical activation of heart in various echocardiographic planes mice, dogs, and humans vivo. By acquiring radio-frequency (RF) frames at very high frame rates (390-520 Hz), onset small, localized, transient deformations resulting from heart, i.e., generating electromechanical (EMW), can be mapped. The correlation between EMW speed pacing scheme previously...

An increased pulse pressure, due to arteries stiffening with age and cardiovascular disease, may lead downstream brain damage in microvessels cognitive decline. Brain-wide imaging of the pulsatility propagation from main feeding capillaries small animals could improve our understanding link between However, it requires higher spatiotemporal resolution penetration depth than currently available existing techniques. Herein, we show feasibility performing Dynamic Ultrasound Localization...

Ultrasound Localization Microscopy (ULM) has proven effective in resolving microvascular structures and local mean velocities at sub-diffraction-limited scales, offering high-resolution imaging capabilities. Dynamic ULM (DULM) enables the creation of angiography or velocity movies throughout cardiac cycles. Currently, these techniques rely on a Localization-and-Tracking (LAT) workflow consisting detecting microbubbles (MB) frames before pairing them to generate tracks. While conventional LAT...

Ultrasound Localization Microscopy (ULM) is a non-invasive technique that allows for the imaging of micro-vessels in vivo, at depth and with resolution on order ten microns. ULM based sub-resolution localization individual microbubbles injected bloodstream. Mapping whole angioarchitecture requires accumulation trajectories from thousands frames, typically acquired over few minutes. acquisition times can be reduced by increasing microbubble concentration, but more advanced algorithms to...

The capability of currently available echocardiography-based strain estimation techniques to fully map myocardial abnormality at early stages ischemia is yet be investigated. In this study, elastography (ME), a radio-frequency (RF)-based imaging technique that maps the full 2D transmural angle-independent tensor in standard echocardiographic views both high spatial and temporal resolution presented. objectives were (1) evaluate performance ME on mapping onset, extent progression graded...