A5037131314- Glycosylation and Glycoproteins Research
- Carbohydrate Chemistry and Synthesis
- Insect symbiosis and bacterial influences
- Mosquito-borne diseases and control
- CRISPR and Genetic Engineering
- Insect Resistance and Genetics
- Plant Virus Research Studies
- Poxvirus research and outbreaks
- Vector-Borne Animal Diseases
- Sphingolipid Metabolism and Signaling
- Ubiquitin and proteasome pathways
- Cancer therapeutics and mechanisms
- Viral Infections and Vectors
- Lysosomal Storage Disorders Research
- Toxin Mechanisms and Immunotoxins
- Bioinformatics and Genomic Networks
- DNA and Nucleic Acid Chemistry
- Animal Genetics and Reproduction
- Alzheimer's disease research and treatments
- Proteoglycans and glycosaminoglycans research
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Vibrio bacteria research studies
- Cancer-related gene regulation
- Viral Infectious Diseases and Gene Expression in Insects
- Insect-Plant Interactions and Control
The Pirbright Institute
2019-2024
University of York
2023
Oxitec (United Kingdom)
2023
Edinburgh Genomics
2023
University of Edinburgh
2023
Virginia Tech
2011-2015
San Francisco General Hospital
2010-2013
University of California, San Francisco
2010-2013
Keele University
2006-2011
University of Manchester
2004-2008
Recent studies suggest that insulin-degrading enzyme (IDE) in neurons and microglia degrades Aβ, the principal component of β-amyloid one neuropathological hallmarks Alzheimer's disease (AD). We performed parametric nonparametric linkage analyses seven genetic markers on chromosome 10q, six which map near IDE gene, 435 multiplex AD families. These revealed significant evidence for adjacent (D10S1671, D10S583, D10S1710, D10S566), was most pronounced late-onset Furthermore, we found...
Sex determination in the mosquito Aedes aegypti is governed by a dominant male-determining factor (M factor) located within Y chromosome-like region called M locus. Here, we show that an M-locus gene, Nix, functions as A. aegypti. Nix exhibits persistent linkage and early embryonic expression, two characteristics required of factor. knockout with clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 resulted largely feminized genetic males production female isoforms key...
I. Fatty acids, neutral fats, long-chain alcohols and bases A. Generic terms (Lip-1.1 -1.5).B. Individual compounds .1. acids (Lip-1.6 -1.7) .2. Sphinganine derivatives (Lip-1.8 -1.11) 3. Glycerol (Lip-1.12-1.13) C. Symbols abbreviations (Lip-1.14-1.18).II.Phospholipids.A. (Lip-2.1 -2.10) B. (Lip-2.11 -2.12) III.Glycolipids.A. General considerations (Lip-3.1 -3.5) .C. (Lip-3.6 -3. 12).D. (Lip-3.12 -3.14).IV.Neuraminic acid (Lip-4.1 -4.3).Appendix A: names of symbols for higher fatty acids.
Two glycosyltransferases were detected in a particulate preparation from 13-day-old embryonic chicken brain. The first enzyme transferred glucose UDP-glucose to ceramide give ceramide-glucose, while the second galactose UDP-galactose ceramide-glucose product partially characterized as ceramide-lactose. These enzymatic steps, addition those previously described brain, delineate pathway for synthesis of disialoganglioside ceramide.
Abstract A particulate preparation from embryonic chicken brain was previously described that catalyzed the transfer of sialic acid CMP-N-acetylneuraminic to galactosyl → glucosyl ceramide give a monosialoganglioside, sialyl (Reaction A). In present studies, two monosialogangliosides served as acceptors in similar reactions, giving corresponding disialogangliosides (Reactions B and C). Reaction yields major disialoganglioside human brain; acceptor is ceramide-pentasaccharide containing...
Significance Mosquitoes are vectors of both parasites and viruses responsible for high-impact diseases including malaria, dengue, chikungunya. Novel interventions based on genetic modification the mosquito genome currently being developed implemented. To comprehensively exploit such interventions, detailed knowledge physiology, genetics, engineering required. We validated a two-step process performing high-efficiency site-specific insertion material into by first evaluating candidate...
Spain has experienced one of the deepest recessions among European countries affected by economic crisis. We investigated effects recession on frequency mental disorders in Primary Care (PC). A group PC physicians selected into study a random sample patients attending primary care centres. These were administered PRIME-MD for assessment disorders, 2006 and again 2010, before during financial Multivariate logistic regression models used to assess relationship unemployment, mortgage payment...
Diseases transmitted by mosquitoes have a devastating impact on global health and this is worsening due to difficulties with existing control measures climate change. Genetically modified that are refractory disease transmission seen as having great potential in the delivery of novel strategies. Historically genetic modification insects has relied upon transposable elements which many limitations despite their successful use. To circumvent these Streptomyces phage phiC31 integrase system...
Lumpy skin disease virus (LSDV) is a vector-transmitted poxvirus that causes in cattle. Vector species involved LSDV transmission and their ability to acquire transmit the are poorly characterized. Using highly representative bovine experimental model of lumpy disease, we fed four vector (Aedes aegypti, Culex quinquefasciatus, Stomoxys calcitrans, Culicoides nubeculosus) on LSDV-inoculated cattle order examine acquisition retention LSDV. Subclinical was more common outcome than clinical...
Lumpy skin disease virus (LSDV) is a poxvirus that causes severe systemic in cattle and spread by mechanical arthropod-borne transmission. This study quantified the acquisition retention of LSDV four species Diptera (Stomoxys calcitrans, Aedes aegypti, Culex quinquefasciatus, Culicoides nubeculosus) from cutaneous lesions, normal skin, blood clinically affected animal. The Ae. aegypti an artificial membrane feeding system was also examined. Mathematical models data were generated to identify...
A particulate preparation from embryonic chicken brain catalyzed the transfer of N-acetylgalactosamine uridine diphospho-N-acetylgalactosamine to ganglioside GM3 (hematoside, sialyllactosylceramide). The kinetic properties transferase were determined. product was isolated and on basis chemical analysis chromatographic behavior shown be Tay-Sachs (GM2). also utilized N-acetyl-D-glucosamine some its derivatives as acceptors, but partial heat inactivation substrate competition experiments...
The N -a c e t y l g u ~~i n r a s f ~ probably involved in the biosynthesis vitro of Ii core glycosphingolipids have been solubilized from membrane preparation mouse lymphoma P-1798 and partially characterized.The detergent-extracted supernatant contains both 81-3-and @1-6-N-acetylglucosaminyltransferase activities that transfer ['HIGlcNAc UDP-~sH]GlcNAc to terminal galactose neolactotetraosylceramide (GalB1-4GlcNAc@l-3GaI@l-4Glc-ceramide; nLcOse4ceramide), form li structures.The linkage [...
An a-fucosyltransferase activity has been detected in a purified membrane preparation isolated from bovine spleen which catalyzes the transfer of L-fucose GDP-L-["Clfucose to tetraglycosylceramide (Lac-nTet-cer, Galpl-4GlcNAc/31-3Gal/?l-4-Glc-cer)to form blood group Hrelated glycosphingolipid.The contained highly active endogenous nonlipid acceptor, could be precipitated by 5% trichloroacetic acid or chloroform-methanol-water (6:3:5, v/v/v), whereas there was little glycosphingolipid...
In September, world leaders will meet at the United Nations in New York to discuss non-communicable diseases.1 A decade ago, a similar meeting on HIV/AIDS, they created Global Fund for Tuberculosis and Malaria—a revolutionary new global health funding mechanism.2 The September focus four leading conditions—heart disease, cancer, diabetes, respiratory disease—that together cause more than half of all deaths low middle income countries.3 Without action, number premature (age < 60) caused by...
The preservation of a homomorphic sex-determining chromosome in some organisms without transformation into heteromorphic sex is long-standing enigma evolutionary biology. A dominant locus (or M-locus) an undifferentiated confers the male phenotype yellow fever mosquito Aedes aegypti. Genetic evidence suggests that M-locus nonrecombining region. However, molecular nature has not been characterized. Using recently developed approach based on Illumina sequencing and female genomic DNA, we...
Multiple viruses, including pathogenic bacteriophages, and even plant cause a phenomenon termed superinfection exclusion whereby currently infected cell is resistant to secondary infection by the same or closely related virus. In alphaviruses, this process thought be mediated, at least in part, viral protease (nsP2) which responsible for processing nonstructural polyproteins (P123 P1234) into individual proteins (nsP1-nsP4), forming replication complex. Taking synthetic biology approach, we...
Morphogens are secreted signaling molecules that form concentration gradients and control cell fate in developing tissues. During development, it is essential morphogen range strictly regulated order for correct type specification to occur. One of the best characterized morphogens Drosophila Decapentaplegic (Dpp), a BMP molecule patterns dorsal ectoderm embryo by activating Mad Medea (Med) transcription factors. We demonstrate there spatial temporal expansion expression Dpp target genes SUMO...
A sialyltransferase activity present in 7-to 12-dayold embryonic chicken brain catalyzes the transfer of sialic acid from CMP-sialic to terminal galactose residue [3H]nLcOse4Cer ([3H]Gal(pl-4)-GlcNAc(pl-3)Gal(pl-4)Glc-Cer) form NeuAc(a2-3)-[3H]nLcOse4Cer (LM1 ganglioside).The product is sialidase-labile (96%), and NeuAc group linked 0-3 residue.The (a2-3) linkage between was determined on basis identification 2,4,6-tri-0methyl["H]galactose obtained after hydrolysis permethylated enzymatic...
Culex quinquefasciatus Say is an opportunistic blood feeder with a wide geographic distribution which also major vector for range of diseases both animals and humans. CRISPR/Cas technologies have been applied to variety organisms basic research purposes. methods open new possibilities genetic in non-model public health importance. In this work we adapted microinjection techniques commonly used other mosquito species quinquefasciatus, shown these be effective at generating homozygous...
A sialyltransferase involved in the biosynthesis vitro of L~1,(NeuAca2-8NeuGca2-3Ga1/91-4Glc-NAc/91-3Gal@1-4Glc-Cer) has been characterized from 9 to 11-day-old embryonic chicken brains.The CMP-[14C]NeuAc:L~l(a2-8)sialyltransferase (SAT-2) sedimented (75%) at junction 0.75 and 1.2 M on a discontinuous sucrose density gradient when still membrane bound.In addition LDlc, detergent-solubilized (0.4% Nonidet P-40) preparation also catalyzes transfer sialic acid 0-8 Gks form G D ~...