A5022680534- MicroRNA in disease regulation
- Circular RNAs in diseases
- Cancer-related molecular mechanisms research
- Fetal and Pediatric Neurological Disorders
- Pregnancy and preeclampsia studies
- Cancer-related gene regulation
- Molecular Biology Techniques and Applications
- interferon and immune responses
- Virus-based gene therapy research
- Congenital Heart Disease Studies
- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Extracellular vesicles in disease
- Adipose Tissue and Metabolism
- RNA modifications and cancer
- MRI in cancer diagnosis
- SARS-CoV-2 and COVID-19 Research
- Intraocular Surgery and Lenses
- Occupational exposure and asthma
- Phytochemical compounds biological activities
- Traditional Chinese Medicine Studies
- Ophthalmology and Visual Impairment Studies
- Wnt/β-catenin signaling in development and cancer
- CO2 Reduction Techniques and Catalysts
- Inflammasome and immune disorders
Army Medical University
2002-2024
Peking University
2024
He Eye Hospital
2024
Tsinghua University
2021-2024
Peking University International Hospital
2024
Southwest Hospital
2024
Affiliated Hospital of Nantong University
2024
Nantong University
2024
Beijing Normal University
2024
Guizhou Academy of Agricultural Sciences
2017-2023
Genistein may regulate lipid metabolism in adipose tissue of obese mice by regulating the expression miR-222 and its target genes, BTG2 adipor1.
Cardiomyocyte hypertrophy, a prevalent clinical condition is deeply associated with many physiological factors. The underlying mechanisms of cardiomyocyte hypertrophy are not yet fully understood. In this study, H9C2 cells were treated genistein, miR-451 mimic, inhibitor and isoproterenol for 24 h, to study the effect genistein on isoproterenol-induced myocardial in vitro. Simultaneously, ICR mice 21 days evaluate effects phytochemical vivo. Results showed that induced cardiac down-regulated...
Significance We established a genome-wide CRISPR library and RNA interference for bat species performed two genetic screens to uncover host factors of cells involved in virus infections. Although the viruses methodologies used were different screens, we identified one common protein, MTHFD1, as broad-spectrum factor. Further studies show that MTHFD1 inhibitor, carolacton, can potently inhibit variety viruses, including SARS-CoV-2. provide resource studying functional genomics biology.
The development of skeletal muscle is a complex process including myoblasts proliferation and differentiation. MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression at post-transcriptional level. Increasing evidences indicate miRNAs important regulators in myogenic processes. Here, we reported the miR-10b-5p steadily decreased during proliferation, but significantly increased over-expression promoted blunted myofiber formation C2C12 cells, while down-regulation showed an...
Adipogenesis is a complex biological process and the main cause of obesity. Recently, microRNAs (miRNAs), class small endogenous non-coding RNAs, have been proven to play an important role in adipogenesis by post-transcriptional regulation target genes. In this current study, we observed increment miR-152 expression during 3T3-L1 cell audiogenic differentiation. A functional analysis indicated that overexpression inhibited pre-adipocyte proliferation suppressed some cycle-related Moreover,...
Functional differences in the different types of adipose tissue and impact their dysfunction on metabolism are associated with regional distribution depots.Here we show a genome-wide comparison between transcriptomes one source subcutaneous two sources visceral pig using an RNA-seq approach.We obtained ~32.3 million unique mapped reads which covered ~80.2% current annotated transcripts across these three tissue.We identified various genes differentially expressed tissue, potentially...
Effective, targeted therapy for chronic liver disease nonalcoholic steatohepatitis (NASH) is imminent. MicroRNAs (miRNAs) are a potential therapeutic target, and natural products that regulate miRNA expression may be safe effective treatment strategy disease. Here, we investigated the functional role of miR-451 effects genistein in NASH mouse model. MiR-451 was downregulated various types inflammation, subsequent experiments showed regulates inflammation via IL1β. Genistein phytoestrogen...
Abstract Although diaphragmatic dysfunction is an important indicator of severity illness and poor prognosis in ICU patients, there no convenient practical method to monitor function. This study was designed analyze dynamic by bedside ultrasound septic patients provide quantitative evidence assess diaphragm function systematically. prospective observational conducted from October 2019 January 2021 the Department Respiratory Critical Care Medicine. 74 suffered sepsis were recruited divided...
Skeletal muscle is the most abundant and a highly plastic tissue of mammals, especially when it comes to regenerate after trauma, but there limited information about mechanism repair its regeneration. In present study, we found that miR-204 downregulated skeletal injury. vitro experiments showed over-expression by transfecting with mimics suppressed C2C12 cell proliferation, migration, blocked subsequent differentiation, whereas inhibition inhibitor converse effects. Furthermore, through...
Carolacton, a naturally occurring MTHFD1 inhibitor, exhibits potent inhibitory activity against various RNA viruses including SARS-CoV-2. Herein, we present concise total synthesis of carolacton, featuring the Krische allylation, Marshall coupling, NHK and RCM reaction as key elements. Additionally, have synthesized three simplified carolacton analogues, one which, namely, 14-demethyl-carolacton, exhibited notable antiviral activity. The work paves way for further exploration therapeutic...
Cardiac hypertrophy is a common pathological condition and an independent risk factor that triggers cardiovascular morbidity. As important epigenetic regulator, miRNA widely involved in many biological processes. In this study, miRNAs expressed rat hearts underwent isoprenaline-induced cardiac were identified using high-throughput sequencing, functional verification of typical was performed primary cardiomyocytes. A total 623 identified, which 33 specifically rats. The enriched pathways...
Gene transcripts or mRNAs and long noncoding RNAs (lncRNAs) are differentially expressed during porcine skeletal muscle development. However, only a few studies have been conducted on transcriptome in pigs based timepoints according to the growth curve for porcine. Here, we investigated gene expression Qingyu at three different stages: inflection point with maximum rate (MGI), of gradually increasing stage rapidly (GRI), slowly (RSI). Subsequently, explored profiles development MGI, GRI RSI...
miRNA is a small non-coding RNA, which plays an important role in diverse biological processes. In the present study, we explore effect of ssc-miR-451 on porcine adipose development and meat quality. We observed that was downregulated during primary adipocyte differentiation. Overexpression inhibited adipogenic differentiation, while inhibition promoted The dual luciferase reporter system indicated Acetyl-CoA carboxylase alpha (ACACA) as target gene ssc-miR-451. Correlation analysis...
To observe Doppler changes in the three main cerebral arteries fetuses with congenital heart defects (CHDs). The pulsatility index (PI) values of anterior artery (ACA), middle (MCA), and posterior (PCA) were prospectively compared 78 CHD normal control fetuses. Correlations between PIs neurodevelopment scores (psychomotor development [PDI] mental [MDI]) assessed. MCA-PI was decreased significantly hypoplastic left syndrome (HLHS). ACA-PI reduced HLHS, left-sided obstructive lesions (LSOLs)...
As the medium of material exchange between mother and fetus, umbilical cord blood is closely connected with fetal development. microRNA (miRNA) has a wide range biological functions high flow characteristics. Small RNA sequencing pig venous (UVB) arterial (UAB) revealed that total 302 miRNAs were identified, 106 22 specifically expressed in UVB UAB, respectively. Using two methods differential expression multiple percentage, it found only 35% highly by analysis modes overlap, but 56.25%...
MiR-222-3р has been implicated in tumor cell proliferation and an important role the differentiation maturation of myogenic cells. However, its skeletal myoblast is still unclear. In this study, we found that miR-222-3р expression increases initially then decreases during C2C12 proliferation. Using synthetic miRNA mimics inhibitors gain- or loss-of-function experiments, snowed overexpression cells promotes represses myofiber formation, while downregulation opposite effect. a prediction...
Co-infections of the porcine reproductive and respiratory syndrome virus (PRRSV) Haemophilus parasuis (HPS) are severe in Chinese pigs, but immune response genes against co-infected with 2 pathogens lungs have not been reported.To understand effect PRRSV and/or HPS infection on expression associated lung function.The immune-related was analyzed using RNA-sequencing bioinformatics. Differentially expressed (DEGs) were detected identified by quantitative real-time polymerase chain reaction...