A5083621379- Autophagy in Disease and Therapy
- Endoplasmic Reticulum Stress and Disease
- Ubiquitin and proteasome pathways
- Microtubule and mitosis dynamics
- Epigenetics and DNA Methylation
- Mitochondrial Function and Pathology
- Polyamine Metabolism and Applications
- RNA modifications and cancer
- Insect behavior and control techniques
- Pancreatic function and diabetes
- NF-κB Signaling Pathways
- Cannabis and Cannabinoid Research
- Phagocytosis and Immune Regulation
- Hydrogen's biological and therapeutic effects
- Cancer-related molecular mechanisms research
- Prostate Cancer Treatment and Research
- Cancer-related gene regulation
- Bioactive natural compounds
- Insect-Plant Interactions and Control
- Biochemical and Molecular Research
- Natural product bioactivities and synthesis
- MicroRNA in disease regulation
- Insect Resistance and Genetics
- Ferroptosis and cancer prognosis
- Genomics, phytochemicals, and oxidative stress
Qingdao Agricultural University
2024
Guangzhou Medical University
2017-2024
Texas A&M University
2016-2020
Hebei Medical University
2018-2020
Third Hospital of Hebei Medical University
2018-2020
Texas A&M Health Science Center
2007-2019
University of Manchester
2018
John Wiley & Sons (United States)
2018
Hudson Institute
2018
Nanchang University
2013-2016
Abstract Background Autophagy is a dynamic process during which isolation membranes package substrates to form autophagosomes that are fused with lysosomes autolysosomes for degradation. Although it agreed the LC3II-associated mature move along microtubular tracks, still in dispute if conversion of LC3I LC3II before fully and subsequent fusion require microtubules. Results We use biochemical markers autophagy collection microtubule interfering reagents test question. show interruption...
Abstract Liver fibrosis and hepatocellular carcinoma (HCC) have worldwide impact but continue to lack safe, low cost, effective treatments. In this study, we show how the simple polyamine spermidine can relieve cancer cell defects in autophagy, which trigger oxidative stress–induced death promote liver HCC. We found that autophagic marker protein LC3 interacted with microtubule-associated MAP1S, positively regulated autophagy flux cells. MAP1S stability was turn by its interaction histone...
Although the fibroblast growth factor (FGF) signaling axis plays important roles in heart development, molecular mechanism by which FGF regulates cardiogenesis is not fully understood.To investigate cardiac progenitor cell differentiation.Using mice with tissue-specific ablation of receptors and receptor substrate 2α (Frs2α) cells, we demonstrate that disruption leads to premature differentiation cells mice. Using embryoid body cultures mouse embryonic stem reveal promotes mesoderm but...
PYCR1 is over-expressed in non-small-cell lung cancer (NSCLC) and its high expression accelerates the progression of NSCLC. However, underlying mechanisms NSCLC remain poorly understood. Our study determined promotion occurrence development vitro vivo. Firstly, patterns tissues cells were by RT-PCR, western blot immunohistochemistry. Then, effects on cell proliferation apoptosis evaluated CCK-8 flow cytomery assays. Finally, we explored up-regulatory microRNAs (miRs) if MAPK pathway involved...
Spermidine (SPD), a naturally occurring polyamine, has been recognized as caloric restriction mimetic that confers health benefits, presumably by inducing autophagy. Recent studies have reported oral administration of SPD protects against liver fibrosis and hepatocarcinogenesis through activation microtubule associated protein 1S (MAP1S)-mediated Nuclear factor (erythroid-derived 2)-like 2 (NRF2) is transcription mediates cellular protection maintaining the cell's redox, metabolic,...
The fibroblast growth factor (FGF) signaling axis plays important roles in heart development. Yet, the molecular mechanism by which FGF regulates cardiogenesis is not fully understood. Using genetically engineered mouse and vitro cultured embryoid body (EB) models, we demonstrate that suppresses premature differentiation of progenitor cells, as well autophagy outflow tract (OFT) myocardiac cells. also promotes mesoderm embryonic stem cells (ESCs) but inhibits cardiomyocyte at later stages....
The mitochondrion-associated protein LRPPRC (leucine-rich pentatricopeptide repeat-containing) interacts with one of the microtubule-associated family members MAP1S (microtubule-associated 1 small form), originally named C19ORF5 (chromosome 19 open reading frame 5), to form a complex. LC3 (light chain 3), mammalian homologue yeast autophagy marker ATG8 and most important markers in cells, helps attachment autophagosomes microtubules for trafficking recruitment substrate mitochondria into...
Abstract Dysfunctional autophagy is associated with tumorigenesis; however, the relationship between two processes remains unclear. In present study, we showed that MAP1S levels immediately become elevated in response to diethylnitrosamine-induced or genome instability-driven metabolic stress a murine model of hepatocarcinoma. Upregulation enhanced remove aggresomes and dysfunctional organelles trigger DNA double-strand breaks instability. The early accumulation an unstable before signs...
MAP1S (originally named C19ORF5) is a widely distributed homolog of neuronal‐specific MAP1A and MAP1B, bridges autophagic components with microtubules mitochondria to affect autophagosomal biogenesis degradation. Mitochondrion‐associated protein LRPPRC functions as an inhibitor for autophagy initiation protect from interact each other may collaboratively regulate although the underlying mechanism yet unknown. Previously, we have reported that levels serve prognosis marker patients prostate...
BACKGROUND Autophagy has recently been found to play important roles in tumorigenesis and leucine‐rich pentatricopeptide repeat motif‐containing protein (LRPPRC) identified as an inhibitor that suppresses autophagy mitophagy maintains mitochondrial activity. The authors hypothesized LRPPRC levels can be used a biomarker for the diagnosis prognosis of prostate cancer. METHODS Immunochemistry analysis was performed evaluate 112 samples collected from patients with adenocarcinoma (PCa) 38...
// Guibin Xu 1, 2, 3, * , Yaodong Jiang 4, Yuansong Xiao 5, Xian-De Liu 6 Fei Yue 3 Wenjiao Li Xun 2 Yongzhong He Xianhan Hai Huang Qi Chen Eric Jonasch Leyuan 7 1 Department of Urology, The Fifth Affiliated Hospital Guangzhou Medical University, Guangzhou, Guangdong Province, 510700, China Center for Innovation and Translation Minimally Invasive Techniques, Institute Biosciences Technology, Texas A&M Health Science Center, Houston, Texas, 77030, USA 4 Nanfang Hospital, Southern 510515, 5...
Autophagy plays an important role in tumorigenesis. Mitochondrion-associated protein LRPPRC interacts with MAP1S that LC3 and bridges autophagy components microtubules mitochondria to affect flux. Dysfunction of is associated poor survival ovarian cancer patients. Furthermore, elevated levels predict shorter overall patients prostate adenocarcinomas or gastric cancer. To understand the tumor development, previously we reported forms a ternary complex Beclin 1 Bcl-2 inhibit autophagy. Here...
Background P62 (also named sequestosome-1, SQSTM1) is involved in autophagy regulation through multiple pathways. It interacts with autophagosomes-associated LC3-II and ubiquitinated protein aggregates to engulf the autophagosomes, HDAC6 inhibit its deacetylase activity maintain levels of acetylated α-tubulin stabilities microtubules enhance autophagosome trafficking, regulates initiation cell survival. We performed immunohistochemistry staining prostate tissues from cancer patients found...
Matrine is an alkaloid from Sophora flavescens that exhibits multiple protective effects on cancers. However, the molecular mechanisms of anti-metastatic matrine castration-resistant prostate cancer (CRPC) remain unknown. This study investigated CRPC to identify underlying mechanisms. The cell viability DU145 and PC-3 cells were measured using MTS assay. impact expression levels matrix metalloproteinase (MMP)-9, MMP-2, nuclear factor-κB (NF-κB) subunit p65 phosphorylated in untreated or...
Background Matrine is a naturally occurring alkaloid extracted from the Chinese herb Sophora flavescens . It has been demonstrated to exhibit antiproliferative properties, promote apoptosis, and inhibit cell invasion in number of cancer lines by modulating NF‐κB pathway downregulate expression MMP2 MM9. also shown improve efficacy chemotherapy when it combined with other drugs. However, therapeutic potential matrine for prostate needs be further studied. Methods We analyzed KEGG pathways...
Abstract Isoform-specific epigenetic silencing of RASSF1A (3p21.3) by promoter-specific CpG island hypermethylation occurs at high frequency in human tumors, whereas the closely related product same gene, RASSF1C, continues to be expressed. Both isoforms isolation exhibit tumor suppressor properties and we show here similar cellular locations on mitochondria microtubules, paclitaxel-like microtubule hyperstabilization, disruption mitosis, interaction with C19ORF5. We both have identical but...
From microscopic observations of autophagosome content it has been argued that autophagy is shut down during mitosis to protect the relative short-lived organelles spindle and chromosomes from process while they are contiguous with cytosol. However, without autophagy, buildup dysfunctional mitochondria arising intense energy demands potentially poses a hazard accurate partition chromosomes. Here we show using biochemical markers autophagosomes mitophagosomes blockade at lysosomal clearance...
Microtubule-associated protein 1 small form (MAP1S; originally named C19ORF5) was identified as serving linkers to connect mitochondria with microtubules for trafficking, and bridge the autophagy machinery affect autophagosomal biogenesis degradation. We found that MAP1S levels become elevated immediately in response diethylnitrosamine-induced or genome instability-driven metabolic stress a murine model of hepatocarcinoma. Elevation enhances remove p62-associated aggresomes dysfunctional...
RASSF1A (Ras association domain family 1 isoform A) is a tumor suppressor and frequently inactivated by promoter hypermethylation in hepatocellular carcinoma (HCC). Autophagy to degrade misfolded or aggregated proteins dysfunctional organelles. defects enhance oxidative stress genome instability promote tumorigenesis. Activating autophagy flux increasing levels of the RASSF1A-interacting microtubule-associated protein S (MAP1S) leads suppression HCC addition extending lifespans. Here we...
Prostate cancer poses a public health threat to hundreds of people around the world. p62 has been identified as tumor suppressor, however, mechanism by which promotes prostate remains poorly understood. The present study aimed investigate whether proliferation, apoptosis resistance and invasion cells via Kelch‑like ECH‑associated protein 1/nuclear factor erytheroid‑derived 2‑like 2/antioxidant response element (Keap1/Nrf2/ARE) axis. Immunohistochemical staining immunoblotting were performed...
Abstract Dissolving the lipid droplets in tissue section with alcohol during a hematoxylin and eosin (H&E) stain causes tumor cells to appear like clear soap bubbles under microscope, which is key pathological feature of cell renal carcinoma (ccRCC). Mitochondrial dynamics have been reported be closely associated metabolism development. However, relationship between mitochondrial reprogramming ccRCC remains further explored. We conducted bioinformatics analysis identify genes regulating...