A5069805233- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Chemokine receptors and signaling
- Immune Response and Inflammation
- Immune cells in cancer
- Asthma and respiratory diseases
- Autoimmune and Inflammatory Disorders Research
- Hematopoietic Stem Cell Transplantation
- interferon and immune responses
- Phagocytosis and Immune Regulation
- Cytokine Signaling Pathways and Interactions
- Atherosclerosis and Cardiovascular Diseases
- Multiple Myeloma Research and Treatments
- Parvovirus B19 Infection Studies
- Dermatology and Skin Diseases
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Immunodeficiency and Autoimmune Disorders
- Multiple Sclerosis Research Studies
- Inflammasome and immune disorders
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Urticaria and Related Conditions
- Systemic Lupus Erythematosus Research
- RNA Research and Splicing
- Neuroblastoma Research and Treatments
Kanazawa Medical University
2017-2023
Tokyo Medical University
2018
The University of Tokyo
1994-2017
Tokyo Medical and Dental University
2009-2017
Japan Science and Technology Agency
2009-2015
Akita University
2007-2009
Kanazawa University
2001
University of California, Los Angeles
2001
University of Vienna
2001
General Department of Preventive Medicine
2001
Flt3 ligand (Flt3L) is a nonredundant cytokine in type I interferon–producing cell (IPC) and dendritic (DC) development, IPC DC differentiation potential confined to Flt3+ hematopoietic progenitor cells. Here, we show that overexpression of human Flt3− (Flt3−Lin−IL-7Rα−Thy1.1−c-Kit+) (Flt3+Lin−IL-7Rα−Thy1.1−c-Kit+) progenitors rescues enhances their potential, respectively. In defined populations, such as megakaryocyte/erythrocyte-restricted (MEPs), enforced signaling induces transcription...
Autophagy is a lysosomal degradation pathway with important roles in physiological homeostasis and disease. However, the role of autophagy intestinal stem cells (ISCs) unclear. Here, we show that intrinsic ISCs for ISC homeostasis. Mice lacking protein 5 (ATG5) epithelial (iECs) (Villin-Cre: Atg5fl/fl, hereafter Atg5ΔIEC mice) or all iECs except Paneth (Ah-Cre: Atg5fl/fl had significantly fewer than did control mice showed impaired ISC-dependent recovery after irradiation. Crypt from higher...
We observed here that the expression of B lymphocyte chemokine (BLC/CXCL13) was markedly enhanced in thymus and kidney aged (NZB × NZW)F1 (BWF1) mice developing lupus nephritis, but not similarly NZB NZW mice. BLC-positive cells were present cellular infiltrates target organs with a reticular pattern staining. CD11b+CD11c+ dendritic increased spleen BWF1 identified as major cell source for BLC. CD4+ T well dramatically mice, whereas no increase B1/B2 ratio significantly higher than those...
Abstract The immunosuppressive and anti-inflammatory cytokine IL-10 inhibits the phenotypic functional maturation of dendritic cells (DC) has been reported to confer tolerogenic properties on these important professional APC. Here, we exposed murine bone marrow-derived myeloid DC either mouse (m) or viral (v) early during their in vitro generation response GM-CSF IL-4. Both mIL-10 vIL-10 down-regulated expression CCR7 mRNA determined by RT-PCR, while up-regulated CCR5 transcripts. These...
Oxidized 1-palmitoyl-2-arachidonoyl- sn -glycero-3-phosphorylcholine (Ox-PAPC) upregulates a spectrum of inflammatory cytokines and adhesion molecules different from those induced by classic mediators such as tumor necrosis factor-α (TNF-α) or lipopolysaccharide. Interestingly, Ox-PAPC also induces the expression set proteins similar to TNF-α lipopolysaccharide, which include chemokines monocyte chemotactic protein-1 (MCP-1) interleukin (IL)-8. To elucidate molecular mechanisms...
Cytotoxic lymphocytes, NK cells and CD8+ T play a pivotal role in the host defense. To reveal biological function of these through establishing comprehensive gene expression profile, serial analysis was performed human peripheral blood cells. In total, 85,848 tags corresponding to >20,000 different transcripts were sequenced. The genes expressed abundantly libraries mostly consisted encoding MHC class I molecules related protein synthesis. Among which cytotoxicity, granulysin, perforin,...
Abstract We have investigated the involvement of chemokine receptor CCR1-positive cells in bleomycin-induced lung injury, a model pulmonary fibrosis. After bleomycin challenge C57BL/6J mice, expression CCR1 mRNA increased and peaked at day 7, which paralleled to its ligands, macrophage-inflammatory protein-1α RANTES. Immunohistochemical study showed that accumulated interstitial inflammatory site. Furthermore, treatment anti-CCR1 Ab significantly reduced accumulation collagen deposition,...
To understand the role of endogenous AP-1 activity in cellular transformation induced by oncogenes, we have made use a fos mutant (supfos-1) and jun (supjun-1), either which can function as transdominant inhibitor AP-1-mediated transcriptional regulation. Chicken embryo fibroblasts (CEF) infected with series transforming retroviruses were doubly retrovirus carrying supfos-1 or supjun-1, suppression was monitored terms reversion to normal morphology acquisition anchorage-dependent growth....
Cryptopatches (CP) are murine gut anatomical sites for generating thymus‐independent intraepithelial T lymphocytes (IEL). However, it remains elusive how lympho‐hematopoietic progenitor cells migrate from bone marrow (BM) into CP and differentiate IEL. Here we show that mice reconstituted with BM‐derived c‐kit+ express CCL25 (TECK)‐intrakine gene, which reduces specifically the chemotactic response to but not CXCL12 in thymocytes. These exhibited a dramatic reduction of IEL small intestine,...
Background: Th2 cells are thought to be involved in eosinophilic inflammation of the lung. CC chemokine receptor 4 (CCR4) has been identified as a specific for both thymus and activation‐regulated (TARC) macrophage‐derived (MDC), is preferentially expressed on cells. Objective: Our aim was evaluate role lung patients with pneumonia (EP). Methods: The concentrations TARC, MDC, interleukin (IL)‐5 were measured bronchoalveolar lavage fluid (BALF) by ELISA. Proportion CCR4‐expressing CD4+ T...
Abstract In vivo steady-state type I natural IFN-producing and dendritic cell (DC) development is largely dependent on Flt3 signaling. Natural DC progenitors their respective downstream populations express the flt3 receptor, ligand (Flt3L)−/− mice have reduced while Flt3L-injected develop markedly increased numbers of both types. present study, we show that SU11657, a small multitargeted receptor tyrosine kinase inhibitor with affinity, suppressed in vitro Flt3L-supplemented mouse whole bone...