Microbial colonization of bone is an important mechanism postmortem skeletal degradation. However, the types and distributions tooth colonizing microbes are not well characterized. It unknown if microbial communities vary in abundance or composition between element types, which could help explain differences human DNA preservation. The goals present study were to (1) identify capable different (2) relate abundances, diversity, community type extracts from 165 samples three skeletonized...

Cortical bone porosity is intimately linked with remodeling, of growing clinical interest, and increasingly accessible by imaging. Thus, the potential animal models osteoporosis (OP) to provide a platform for studying how develops responds interventions tremendous. To date, rabbit OP have largely focused on trabecular microarchitecture or density; some such as ovariectomy (OVX) uncertain efficacy cortical has not been extensively reported. Our primary objective was characterize tibial in...

Abstract Objectives Differentiating human from nonhuman fragmented bone is often accomplished using histological methods if the observation of gross morphology proves insufficient. Linearly oriented primary and/or secondary osteonal systems, commonly referred to as osteon bands, are described a strong indicator bone, particularly occurrence multiple bands. This phenomenon has been conventionally documented two‐dimensional (2D) histology, but such analyses destructive and typically limited...

Bone is a mechanically active, three-dimensionally (3D) complex, and dynamic tissue that changes in structure over the human lifespan. exists remodels 3D time, introducing fourth dimension. The products of remodelling process, secondary fragmentary osteons, have been studied substantially using traditional two-dimensional (2D) techniques. As result, much has learned regarding biological information encrypted histomorphology bone, yielding wealth relating to skeletal function....

X-ray photoelectron spectroscopy was used to evaluate differences in elemental composition of human bone tissue types and identify soil infiltration.

Abstract Microbial colonization of bone is an important mechanism post-mortem skeletal degradation. However, the types and distributions tooth colonizing microbes are not well characterized. It unknown if microbial communities vary in abundance or composition between element types, which could help explain patterns human DNA preservation. The goals present study were to (1) identify capable different (2) relate abundances, diversity, community type extracts from 165 samples three...

Abstract Bones are dynamic living organs that undergo continual change throughout life. An internal process of tissue renewal, called remodeling, removes mature microscopic packets bone, and replaces them with new bone in a highly coordinated manner. To date, it remains difficult to directly observe track individual remodeling events cortical due the small size structures involved. High‐resolution imaging techniques hold potential provide novel three‐dimensional information pertaining...

Cellular communication in the mechanosensory osteocyte Lacuno-Canalicular Network (LCN) regulates bone tissue remodeling throughout life. Age-associated declines LCN size and connectivity dysregulate mechanosensitivity to localized needs of aging or damaged tissue, compromising quality. Synchrotron radiation-based micro-Computed Tomography (SRμCT) Confocal Laser Scanning Microscopy (CLSM) were employed visualize vascular canal morphometry an age series anterior femur (males n = 14, females...

Abstract Quantifying the amount of cortical bone loss is one variable used in histological methods adult age estimation. Measurements area tend to be subjective and additional information regarding not captured considering cancellous disregarded. We describe whether measuring (cancellous + area) rather than may improve estimation for sixth rib. Mid‐shaft rib cross‐sections ( n = 114) with a skewed sex distribution were analyzed. Ages range from 16 87 years. Variables included: total...

Bone is a dynamic and mechanically active tissue that changes in structure over the human lifespan. The products of bone remodeling process have been studied substantially using traditional two-dimensional techniques. Recent advancements X-ray imaging technology via desktop micro-computed tomography (µCT) synchrotron radiation (SRµCT) allowed for acquisition high-resolution three-dimensional (3D) scans larger field view (FOV) than other 3D techniques (e.g., SEM) providing more complete...

The hypothesis advanced is that endospanin, a highly conserved vesicle traffic protein in vertebrates, regulates leptin sensitivity bone signaling. effects of on bones are well-studied but without consensus whether the increases signaling stimulate gain or loss. response may depend sensitivity, and endospanin an established modulator sensitivity. An argument to develop zebrafish models for specific pathways. Zebrafish have well-developed molecular tools (e.g., CRISPR) advantage...

Abstract We examined the ultrastructure of mammalian os penis at high‐resolution synchrotron level. Previously, bacular microanatomy had only been investigated histologically. studied baculum harp seal ( Pagophilus groenlandicus ), in which varies more size and shape than does a mechanically constrained bone (humerus). (1) microarchitecture bacula humeri from same specimens, (2) described changes micro‐ macro‐morphology associated with age n = 15, range 1–35 years) type. analyzed...

Due to the high prevalence of opioid prescription following orthopedic procedures, there is a growing need establish an animal model system evaluate effects opioids on bone remodeling. Rabbits have been employed as organisms in research they exhibit well-defined cortical remodeling similar humans. Existing rabbits has limited modes administration that are short-acting and require repeated application. Here, we present data from proof-of-principle longitudinal study employing two analgesic...

The Andronowski Skeletal Collection for Histological Research (ASCHR) comprises the fastest-growing documented modern human skeletal collection in world developed specifically histological and imaging research. Initiated 2017 by Dr. Janna M. Andronowski, ASCHR provides a resource study of microarchitectural variability with advancing age between sexes. primary objective is to use this unique archive research, goal furthering knowledge bone biology. Bone procurement has focused on two sites...

Bats are the only mammals to have achieved powered flight. A key innovation allowing for bats conquer skies was a forelimb modified into flexible wing. The wing bones of exceptionally long and dynamically bend with wingbeats. Bone microarchitectural features supporting these novel performance attributes still largely unknown. humeri femora typically avascular, except large-bodied taxa (e.g., pteropodid flying foxes). No thorough investigation vascular canal regionalization morphology has...