A5047388841- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Chronic Myeloid Leukemia Treatments
- Macrophage Migration Inhibitory Factor
- Cancer Research and Treatments
- Cell Adhesion Molecules Research
- Immune Cell Function and Interaction
- Bone health and osteoporosis research
- Hedgehog Signaling Pathway Studies
- Genetic and Kidney Cyst Diseases
- Cancer Immunotherapy and Biomarkers
- Bone Metabolism and Diseases
- Immune cells in cancer
- Microtubule and mitosis dynamics
- Structural Behavior of Reinforced Concrete
- Epigenetics and DNA Methylation
- Peptidase Inhibition and Analysis
- Retinoids in leukemia and cellular processes
- Melanoma and MAPK Pathways
- Developmental Biology and Gene Regulation
- CAR-T cell therapy research
- Neurogenesis and neuroplasticity mechanisms
- Bone health and treatments
- Osteoarthritis Treatment and Mechanisms
- Phagocytosis and Immune Regulation
- Genomics and Chromatin Dynamics
Baby Memorial Hospital
2024
NGM Biopharmaceuticals (United States)
2020-2024
University of Saskatchewan
2017-2023
University of California, San Francisco
2006-2010
Virginia Commonwealth University
2010
Genentech
2007-2009
Ernest Gallo Clinic and Research Center
2005-2009
Harvard University
2003
University of British Columbia
1997-2002
Our expression studies of bone morphogenetic proteins (BMPs) and Noggin (a BMP antagonist) in the embryonic chicken face suggested that signals were important for closure upper lip or primary palate. We noted was restricted to frontonasal mass epithelium but reduced at corners (globular processes) just prior fusion with adjacent maxillary prominences. therefore performed gain- loss-of-function experiments determine role BMPs formation. treatment led proliferation outgrowth prominences...
The lacunar-canalicular network (LCN) of bone contains osteocytes and their dendritic extensions, which allow for intercellular communication, are believed to serve as the mechanosensors that coordinate processes modeling remodeling. Imbalances in remodeling, example, linked disease, including fragility associated with aging. We have reported there is a reduction scale one component LCN, osteocyte lacunar volume, across human lifespan females. In present study, we explore hypothesis...
Embryonic facial development in chick embryos involves a sequential activation of genes that control differential growth and patterning the beak. In present study we isolate one such gene, transcription factor, AP-2, is known to be expressed face mouse embryos. The protein sequence AP-2α 94% homologous human AP-2. Wholemountin situhybridization with probe for AP-2 identifies expression from primitive streak stages up stage 28. most striking patterns head are during neural crest cell...
Regulatory factor X (Rfx) homologs regulate the transcription of genes necessary for ciliogenesis in invertebrates and vertebrates. Primary cilia are Hedgehog signaling regulation activity transcriptional regulators known as Gli proteins, which targets signaling. Here, we describe an Rfx4(L298P) mouse mutant with distinct dorsoventral patterning defects ventral spinal cord telencephalon due to aberrant Sonic hedgehog (Shh) Gli3 activity. We find that Ift172, encodes intraflagellar transport...
Cdc2l1is a component of the Hh signaling pathway and opposes activity negative regulator Sufu.
We recently reported that resistance to PD-1 blockade in a refractory lung cancer-derived model involved increased collagen deposition and the collagen-binding inhibitory receptor leukocyte-associated immunoglobulin-like 1 (LAIR1). Thus, we hypothesized LAIR1 cooperated suppress therapeutic response. In this study, report is associated with tumor stroma highly expressed by intratumoral myeloid cells both human tumors mouse models of cancer. Stroma-associated exhibit suppressive phenotype...
Bone morphogenetic proteins (BMPs) signal via complexes of type I and II receptors. In this study, we mapped the expression IA, IB receptors during craniofacial chondrogenesis then perturbed receptor function in vivo with retroviruses expressing dominant-negative or constitutively active BmprIB was only expressed within all cartilages. BmprIA initially cartilage condensations, but later decreased elements. BmprII at low levels nasal septum prenasal higher other The maxillary prominence,...
Mouse mutants have allowed us to gain significant insight into axis development. However, much remains be learned about the cellular and molecular basis of early forebrain patterning. We describe a lethal mutation mouse strain generated using promoter-trap mutagenesis. The exhibit severe truncation in homozygous embryos various craniofacial defects heterozygotes. show that are caused by disruption gene encoding nucleic acid binding protein (CNBP); Cnbp transgenic mice were able rescue fully...
Precise regulation of Wnt signaling is important in many contexts, as development the vertebrate forebrain, where excessive or ectopic leads to severe brain defects. Mutation widely expressed oto gene causes loss anterior forebrain during mouse embryogenesis. Here we report that ortholog gpi deacylase pgap1, and endoplasmic reticulum (ER)-resident Oto protein has a novel deacylase-independent function maturation. increases hydrophobicities Wnt3a Wnt1 by promoting addition...
Cortical bone porosity is intimately linked with remodeling, of growing clinical interest, and increasingly accessible by imaging. Thus, the potential animal models osteoporosis (OP) to provide a platform for studying how develops responds interventions tremendous. To date, rabbit OP have largely focused on trabecular microarchitecture or density; some such as ovariectomy (OVX) uncertain efficacy cortical has not been extensively reported. Our primary objective was characterize tibial in...
The mechanisms by which ethanol and inhaled anesthetics influence the nervous system are poorly understood. Here we describe positional cloning characterization of a new mouse mutation isolated in an N-ethyl-N-nitrosourea (ENU) forward mutagenesis screen for animals with enhanced locomotor activity. This allele, Lightweight (Lwt), disrupts homolog Caenorhabditis elegans (C. elegans) unc-79 gene. While Lwt/Lwt homozygotes perinatal lethal, heterozygotes dramatically hypersensitive to acute...
Interleukin-1α (IL-1α) is a powerful activator of osteoclast cells. However, the underlying mechanism for this activation unknown. In study, we reveal that IL-1α up-regulates expression cathepsin K protein, key protease in bone resorption, by five-fold. Northern blot analysis and promoter show induction occurs at transcriptional level, dose-responsive time-dependent manner. No increase presence either pyrrolidine dithiocarbamate (PDTC), selective inhibitor NF-κB, or Genistein, protein...
Solid tumors are dense three-dimensional (3D) multicellular structures that enable efficient receptor-ligand trans interactions via close cell-cell contact. Immunoglobulin-like transcript (ILT)2 and ILT4 related immune-suppressive receptors play a role in the inhibition of myeloid cells within tumor microenvironment. The relative contribution ILT2 to immune context solid tissue has not been fully explored. We present evidence both contribute inhibition. found although inhibits cell...
Abstract Ancestors of the Antarctic icefishes (family Channichthyidae) were benthic and had no swim bladder, making it energetically expensive to rise from ocean floor. To exploit water column, benthopelagic hypothesized have evolved a skeleton with “reduced bone,” which gross anatomical data supported. Here, we tested hypothesis that changes icefish bones also occurred below level anatomy. Histology micro‐CT imaging representative craniofacial (i.e., ceratohyal, frontal, dentary, articular)...
<p>Supplementary Methods, Tables, and Figure Legends</p>
<p>Supplementary Figure S5</p>
<p>Supplementary Figure S8</p>
<p>Supplementary Methods, Tables, and Figure Legends</p>