A5100631140- Hedgehog Signaling Pathway Studies
- Epigenetics and DNA Methylation
- Developmental Biology and Gene Regulation
- Genetic and Kidney Cyst Diseases
- Ocular Disorders and Treatments
- Congenital limb and hand anomalies
- Congenital Ear and Nasal Anomalies
- Wnt/β-catenin signaling in development and cancer
- Genomics and Chromatin Dynamics
- Enterobacteriaceae and Cronobacter Research
- Bone Metabolism and Diseases
- Genetic and rare skin diseases.
- RNA Interference and Gene Delivery
- Tissue Engineering and Regenerative Medicine
- Escherichia coli research studies
- Cellular Mechanics and Interactions
- Connective tissue disorders research
- Head and Neck Surgical Oncology
- Viral Infectious Diseases and Gene Expression in Insects
- Click Chemistry and Applications
- Bone health and treatments
- Skin and Cellular Biology Research
Cornell University
2007-2023
University of Wyoming
2020
Gansu Provincial Maternal and Child Health Hospital
2016
University of Miami
2015
Dartmouth College
2015
Army Medical University
2015
Southwest Hospital
2015
Molecular Oncology (United States)
2015
University of Alabama at Birmingham
2015
Sylvester Comprehensive Cancer Center
2015
Primary cilia are present on mammalian neurons and glia, but their function is largely unknown. We generated conditional homozygous mutant mice for a gene we termed Stumpy. Mutants lack have conspicuous abnormalities in postnatally developing brain regions, including hypoplasic hippocampus characterized by primary deficiency neural stem cells known as astrocyte-like precursors (ALNPs). Previous studies suggested that mediate sonic hedgehog (Shh) signaling. Here, find loss of ALNP leads to...
Hedgehog-regulated processing of the transcription factor cubitus interruptus (Ci) in Drosophila depends on phosphorylation C-terminal region Ci by cAMP-dependent protein kinase and subsequently casein 1 glycogen synthase 3. also requires Slimb, an F-box SCF ( S kp1/ C ullin/ F -box proteins) complex, proteasome, but interplay between activity Slimb proteasome remains unclear. Here we show that Gli3 protein, a homolog Ci, set four sites primes subsequent adjacent Our gain- loss-of-function...
Budding in Saccharomyces cerevisiae follows a genetically programmed pattern of cell division which can be regulated by external signals. On the basis known functional conservation between number mammalian oncogenes and antioncogenes with genes yeast budding pathway, we used enhancement pseudohyphal S. human proteins expressed from HeLa cDNA library as morphological screen to identify candidate that coordinate cellular signaling morphology. In this report, describe isolation characterization...
The amino-terminal signaling domain of the Sonic hedgehog secreted protein (Shh-N), which derives from Shh precursor through an autoprocessing reaction mediated by carboxyl-terminal domain, executes multiple functions in embryonic tissue patterning, including induction ventral and suppression dorsal cell types developing neural tube. An apparent catalytic site within Shh-N is suggested structural homology to a bacterial carboxypeptidase. We demonstrate here that alteration residues presumed...
Loss of the kinase and tumor suppressor Stk11 (Lkb1) has opposite effects on Hh Wnt signaling.
AbstractBone morphogenetic protein 2 (BMP-2) is essential for postnatal bone formation and fracture repair. By screening chemical libraries BMP-2 mimics using a cell-based assay, we identified inhibitors of microtubule assembly as stimulators transcription. These increased osteoblast differentiation in vitro, stimulated periosteal when injected locally over murine calvaria, enhanced trabecular administered systemically vivo. To explore molecular mechanisms mediating these responses, examined...
The inner ear consists of two otocyst-derived, structurally and functionally distinct components: the dorsal vestibular ventral auditory compartments. BMP signaling is required to form compartment, but how it complements other molecules acts intracellularly unknown. Using spatially temporally controlled delivery pathway regulators developing chick otocysts, we show that regulates expression Dlx5 Hmx3, both which encode transcription factors essential for formation. However, while through...
A fundamental problem in tissue morphogenesis is identifying how subcellular signaling regulates mesoscale organization of tissues. The primary cilium a paradigmatic organelle for compartmentalized signaling. How emanating from cilia orchestrates organization-especially, the role cilia-generated effectors mediating diverse morpho-phenotypic outcomes-is not well understood. In hedgehog pathway, bifunctional GLI transcription factors generate both GLI-activators (GLI-A) and GLI-repressors...
Abstract Gli3 protein processing to generate the repressor is mediated by proteasome and inhibited Hedgehog signaling. The concentration graded along anterior–posterior axis of developing vertebrate limb due posteriorly restricted Sonic hedgehog expression. In this study, we created a small deletion at locus ( Δ 68 ), which causes half reduction in levels slightly increased activity full‐length mutant limb. Mice homozygous for develop one two extra partial digits anterior limb, while mice...
The metalloid arsenic is a worldwide environmental toxicant, exposure to which associated with many adverse outcomes. Arsenic also an effective therapeutic agent in certain disease settings. was recently shown regulate the activity of Hedgehog (HH) signal transduction pathway, and this regulation HH signaling proposed be responsible for subset arsenic’s biologic effects. Surprisingly, these separate reports contradictory activities arsenic, as either agonist or antagonist signaling. Here we...
To construct eukaryotic expression vector of siRNA specific for BCR/ABL and to investigate the effect recombinant plasmid on P210 protein in K562 cells.siRNA(small interfering RNA)was designed according Tuschl's principle Ai-based medicine, was converted into cDNA coding shRNA(small hairpin RNAs)of fusion gene. The synthesized inserted pTER. pTER117 pTER363 being controlled by H1 promoter RNA polymerase III, identified restriction map sequence analysis. did not only have screening resisting...
Objective: Non-syndromic Mitral Valve Prolapse (MVP) is a common disease with associated morbidities and mortality. The cause of the poorly understood to date only couple genes have been shown as causative. We recently identified mutations in cilia gene, DZIP1 families MVP. To initially identify function valve development, we performed proteomics based approaches goal identifying unique binding partners for DZIP1. These studies revealed direct interaction beta-catenin...