A5034986244- Immunotherapy and Immune Responses
- Immune Response and Inflammation
- Streptococcal Infections and Treatments
- Neonatal and Maternal Infections
- Antimicrobial Resistance in Staphylococcus
- Influenza Virus Research Studies
- Viral gastroenteritis research and epidemiology
- Immune Cell Function and Interaction
- RNA Interference and Gene Delivery
- Respiratory viral infections research
- Platelet Disorders and Treatments
- interferon and immune responses
- Antimicrobial Peptides and Activities
- SARS-CoV-2 and COVID-19 Research
- Bacterial Infections and Vaccines
- Escherichia coli research studies
- Blood groups and transfusion
- Hepatitis B Virus Studies
- Advancements in Transdermal Drug Delivery
- Animal Virus Infections Studies
- Pediatric health and respiratory diseases
- Research on Leishmaniasis Studies
- Hepatitis C virus research
- Advanced biosensing and bioanalysis techniques
- T-cell and B-cell Immunology
Helmholtz Centre for Infection Research
2015-2024
University Medical Center of the Johannes Gutenberg University Mainz
2022
Johannes Gutenberg University Mainz
2022
Robert Koch Institute
2021
University of Brescia
2014
National Institutes of Health
2014
National Cancer Institute
2014
University of Calabria
2014
Manchester Metropolitan University
2014
Universität Hamburg
2008
Platelet (PLT) transfusion is indispensable to maintain homeostasis in thrombocytopenic patients. However, PLT refractoriness a common life-threatening condition observed multitransfused The most frequent immune cause for the presence of alloantibodies specific human leukocyte antigen (HLA) class I epitopes. Here, we have silenced expression HLA generate stable HLA-universal induced pluripotent stem cell (iPSC) line that can be used as renewable source generation low immunogenic products....
To explore mechanisms of hepatitis C viral (HCV) replication we screened a compound library including licensed drugs. Flunarizine, diphenylmethylpiperazine used to treat migraine, inhibited HCV cell entry in vitro and vivo genotype‐dependent fashion. Analysis mosaic viruses between susceptible resistant strains revealed that E1 E2 glycoproteins confer susceptibility flunarizine. Time addition experiments single particle tracking demonstrated flunarizine specifically prevents membrane fusion....
Bordetella petrii is the only environmental species hitherto found among otherwise host-restricted and pathogenic members of genus Bordetella. Phylogenetically, it connects Bordetellae bacteria genera Achromobacter Alcaligenes, which are opportunistic pathogens. B. strains have been isolated from very different niches, including river sediment, polluted soil, marine sponges a grass root. Recently, clinical isolates associated with bone degenerative disease or cystic fibrosis also described....
Group A streptococci (GAS) can cause a wide variety of human infections ranging from asymptomatic colonization to life-threatening invasive diseases. Although antibiotic treatment is very effective, when left untreated, Streptococcus pyogenes lead poststreptococcal sequelae and severe disease causing significant morbidity mortality worldwide. To aid the development non-M protein-based prophylactic vaccine for prevention group streptococcal infections, we identified novel immunogenic proteins...
The parasite Trypanosoma cruzi is the causative agent of Chagas disease, a potentially life-threatening infection that represents major health problem in Latin America. Several characteristics this protozoan contribute to lack an effective vaccine, among them: its silent invasion mechanism, T. antigen redundancy and immunodominance without protection. Taking into account these issues, we engineered Traspain, chimeric tailored present multivalent display domains from key parasitic molecules,...
Nucleic acid vaccination relies on injecting DNA or RNA coding antigen(s) to induce a protective immune response. is being increasingly used in preclinical and clinical studies. However, few delivery systems have been reported for vivo of different sizes. Using tripartite formulation with RNA, cationic polymer, anionic liposomes, we were able encapsulate into neutral lipopolyplexes (LPPs). LPPs stable vitro successfully delivered conventional replicative dendritic cells cellulo. Their...
The development of mucosal adjuvants is still a critical need in vaccinology. In the present work, we show that bis(3',5')-cyclic dimeric GMP (cdiGMP), second messenger modulates cell surface properties several microorganisms, exerts potent activity as adjuvant. BALB/c mice were immunized intranasally with model antigen beta-galactosidase (beta-Gal) coadministered cdiGMP. Animals receiving cdiGMP an adjuvant showed significantly higher anti-beta-Gal immunoglobulin G (IgG) titers sera than...
Refractoriness to platelet (PLT) transfusion caused by alloimmunization against HLA class I antigens constitutes a significant clinical problem. Thus, it would be desirable have PLT units lacking on the cell surface. Previously, we showed that generation of functional I-silenced (HLA-universal) PLTs from CD34(+) cells, using short hairpin RNA (shRNA) target β2-microglobulin (β2m) transcripts, is feasible. Here, assessed capacity HLA-silenced escape antibody-mediated cytotoxicity in vitro and...
The need for more effective influenza vaccines is highlighted by the emergence of novel strains, which can lead to new pandemics. There a growing population susceptible subjects at risk severe complications influenza, such as elderly who are only in part protected current licensed seasonal vaccines. One strategy improving and pandemic takes advantage adjuvants boost modulate evoked immune responses. In this study, we examined capacity recently described adjuvant cyclic di-adenosine...
There is a critical need for new influenza vaccines able to protect against constantly emerging divergent virus strains. This will be sustained by the induction of vigorous cellular responses and humoral immunity capable acting at portal entry this pathogen. In study we evaluate protective efficacy intranasal vaccination with recombinant nucleoprotein (rNP) co-administrated bis-(3′,5′)-cyclic dimeric adenosine monophosphate (c-di-AMP) as adjuvant. Immunization BALB/c mice two doses...
The development of new effective vaccines strongly depends on adjuvants and formulations able to stimulate not only strong humoral responses against a certain pathogen but also effector as well memory CD4+ CD8+ T cells (Dubensky et al., 2013). However, the majority licensed for human use or currently under clinical investigation fail efficient cellular responses. For example, hepatitis B virus (HBV), papillomavirus (HPV), diphtheria, tetanus influenza are usually administered by...
Vaccination is the most efficient strategy to protect from infectious diseases and induction of a protective immune response not only depends on nature antigen, but also influenced by vaccination co-administration adjuvants. Therefore, precise monitoring adjuvant candidates their modulatory properties crucial step in vaccine development. Here, one central aspect appropriate humoral cellular effector mechanisms. In our study we performed direct comparison two promising development, STING...
AIDS-related lymphomas are high grade and aggressively metastatic with poor prognosis. Lymphangiogenesis is essential in supporting proliferation survival of lymphoma, as well tumor dissemination. Data suggest that aberrant lymphangiogenesis relies on action HIV-1 proteins rather than a direct effect the virus itself. matrix protein p17 was found to accumulate persist lymph nodes patients even under highly active antiretroviral therapy. Because recently exert potent proangiogenic activity by...
The development of new adjuvants enables fine modulation the elicited immune responses. Ideally, use one or more should result in induction a protective response against specific pathogen. We have evaluated and protection Trypanosoma cruzi infection mice vaccinated with recombinant Tc52 its N- C-terminal domains (NTc52 CTc52) adjuvanted either STING (Stimulator Interferon Genes) agonist cyclic di-AMP (c-di-AMP), pegylated derivative α-galactosylceramide (αGC-PEG), oligodeoxynucleotides...
Advances in RNA technology during the past two decades have led to construction of replication-competent RNA, termed replicons, RepRNA, or self-amplifying mRNA, with high potential for vaccine applications. Cytosolic delivery is essential their translation and self-replication, without infectious progeny generation, providing levels antigen expression inducing humoral cellular immunity. Synthetic nanoparticle-based vehicles can both protect molecules facilitate targeting dendritic...