A5071614367- Enzyme Production and Characterization
- Sperm and Testicular Function
- Bacterial Infections and Vaccines
- Streptococcal Infections and Treatments
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Biofuel production and bioconversion
- Fungal and yeast genetics research
- Monoclonal and Polyclonal Antibodies Research
- Bacteriophages and microbial interactions
- Reproductive Biology and Fertility
- Microbial Metabolites in Food Biotechnology
- Glycosylation and Glycoproteins Research
- Pneumonia and Respiratory Infections
- Transgenic Plants and Applications
- Neonatal and Maternal Infections
- Enzyme Structure and Function
- Antimicrobial Resistance in Staphylococcus
- Cellular transport and secretion
- Trace Elements in Health
- Fermentation and Sensory Analysis
- Aquatic and Environmental Studies
- Peptidase Inhibition and Analysis
- Magnesium in Health and Disease
- Forensic and Genetic Research
- Anthropological Studies and Insights
Vietnam Academy of Science and Technology
2021-2024
BOKU University
2015
Vienna Biocenter
1996-2010
Intercell (Austria)
2005-2009
University of Vienna
1996-2001
Bio Forschung Austria
2001
Leibniz Institute for Neurobiology
1996
Leibniz Institute of Plant Genetics and Crop Plant Research
1995-1996
National Heart Lung and Blood Institute
1996
National Institutes of Health
1996
Pneumococcus is one of the most important human pathogens that causes life-threatening invasive diseases, especially at extremities age. Capsular polysaccharides (CPSs) are known to induce protective antibodies; however, it not feasible develop CPS-based vaccines cover all 90 disease-causing serotypes. We applied a genomic approach and described antibody repertoire for pneumococcal proteins using display libraries expressing 15–150 amino acid fragments pathogen's proteome. Serum antibodies...
For the design of potent subunit vaccines, it is paramount importance to identify all antigens immunologically recognized by a patient population infected with pathogen. We have developed rapid and efficient procedure such commonly antigens, here we provide comprehensive in vivo antigenic profile Staphylococcus aureus , an important human S. peptides were displayed on surface Escherichia coli via fusion one two outer membrane proteins (LamB FhuA) probed sera selected for high Ab titer...
The yeast nuclear gene MRS2 encodes a protein of 54 kDa, the presence which has been shown to be essential for splicing group II intron RNA in mitochondria and, independently, maintenance functional respiratory system. Here we show that product (Mrs2p) is an integral inner mitochondrial membrane. It appears inserted into this membrane by virtue two neighboring spanning domains its carboxyl-terminal half. A large amino-terminal and shorter part are likely exposed matrix space. Structural...
There are two isoforms of the vertebrate nonmuscle myosin heavy chain, MHC-A and MHC-B, that encoded by separate genes. We compared enzymatic activities as well subcellular localizations these in Xenopus cells. MHC-B were purified from cells immunoprecipitation with isoform-specific peptide antibodies followed elution their cognate peptides. Using an vitro motility assay, we found velocity movement actin filaments was 3.3-fold faster than MHC-B. Likewise, Vmax actin-activated Mg(2+)-ATPase...
Group A streptococci (GAS) can cause a wide variety of human infections ranging from asymptomatic colonization to life-threatening invasive diseases. Although antibiotic treatment is very effective, when left untreated, Streptococcus pyogenes lead poststreptococcal sequelae and severe disease causing significant morbidity mortality worldwide. To aid the development non-M protein-based prophylactic vaccine for prevention group streptococcal infections, we identified novel immunogenic proteins...
Significance The broadly neutralizing anti–HIV-1 monoclonal antibody (mAb) PG9 requires multiple posttranslational modifications to exhibit its full biological activity, including proper N -glycosylation and tyrosine sulfation. We now describe a technology that permits the controlled synthesis of these in Nicotiana benthamiana . This allowed us show sulfated neutralizes HIV-1 with much higher potency than unsulfated antibody. also found glycooptimized mAb versions made plants are superior...
Passive antibody therapy for cancer is an effective but costly treatment modality. Induction of therapeutically potent anticancer antibodies by active vaccination attractive alternative has proven challenging in due to tolerogenic pressure patients. Here, we used the clinically relevant target Her2, known be susceptible targeting therapy, demonstrate how can induced vaccination. A novel 44kD Her2 protein fragment was generated and found highly at inducing anti-Her2 including trastuzumab-like...
ABSTRACT Four outer membrane proteins of Escherichia coli were examined for their capabilities and limitations in displaying heterologous peptide inserts on the bacterial cell surface. The T7 tag or multiple copies myc epitope inserted into loops 4 5 ferrichrome phage T5 receptor FhuA. Fluorescence-activated sorting analysis showed that peptides up to 250 amino acids efficiently displayed surface E. as within Strains expressing FhuA fusion behaved similarly those wild-type FhuA, judged by...
Abstract Austronesian (AN) is the second-largest language family in world, particularly widespread Island Southeast Asia (ISEA) and Oceania. In Mainland (MSEA), groups speaking these languages are concentrated highlands of Vietnam. However, our knowledge spread AN-speaking populations MSEA remains limited; particular, it not clear if AN were by demic or cultural diffusion. this study, we present analyze new data consisting complete mitogenomes from 369 individuals 847 Y-chromosomal single...
Antioxidant genes, such as superoxide dismutase (SOD), catalase (CAT), and nitric oxide synthase (NOS), play critical roles in spermatogenesis sperm functions. Polymorphisms of antioxidant genes have been shown to be strongly associated with quality which affects male fertility.To investigate the association gene polymorphisms infertility Vietnamese men, this case-control study, using Sanger sequencing, we genotyped four variants SOD1:7958G>A, SOD2:c.47T>C, CAT:-262C>T, NOS3:-786C>T.We...
The Rab escort protein (REP) is an essential component of the heterotrimeric enzyme geranylgeranyl transferase that modifies carboxy-terminal cysteines Ras-like small G proteins belonging to Rab/Ypt family. Deletions in human CHM locus, encoding one two REPs known humans, result a retinal degenerative syndrome called choroideremia. only yeast homologue choroideremia gene product encoded by MRS6. Besides three structurally conserved regions (SCRs) previously detected amino-terminal half and...
Background: The total number of (road) traffic accidents in Vietnam remains high and the death toll caused by these also ranks second Southeast Asia. Many studies elsewhere have shown a positive correlation between risk perception risky behavior (henceforth, referred to as TRP TRB, respectively, for short) However, this relationship has been relatively under-researched context Vietnam. This study aimed fulfill above research gap investigating present status TRB among Vietnamese people who...
Spermatogenesis is a process of cell differentiation to produce fertilized sperm. Testis-expressed 15 (TEX15), an important gene in spermatogenesis, has been reported be linked with male infertility various populations. This study aimed assess the association single nucleotide polymorphism (SNP) TEX15 rs323347 infertile individuals Vietnamese cohort and further analyze haplotypes rs323346 rs323347. A total 401 unrelated males, including 202 patients 199 healthy controls were genotyped for...
Austronesian (AN) is the second-largest language family in world, particularly widespread Island Southeast Asia (ISEA) and Oceania. In Mainland (MSEA), groups speaking these languages are concentrated highlands of Vietnam. However, our knowledge spread AN-speaking populations MSEA remains limited; particular, it not clear if AN were by demic or cultural diffusion. this study, we present analyze new data consisting complete mitogenomes from 369 individuals 847 Y-chromosomal single nucleotide...
Male infertility is a reproductive issue involving defects in the quantity and quality of sperm. Besides exogenous elements such as harmful habits, pollution, genetic factors including disorders single gene, group genes, or chromosomes, are etiology causing men. NR5A1 well-known candidate gene involved male infertility, 46, XY sex development (DSD), cryptorchidism, micropenis, spermatogenic failure, non-obstructive azoospermia, oligozoospermia. This study aimed to identify nucleotide...