A5101784963- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Animal Genetics and Reproduction
- Renal and related cancers
- Genetic and phenotypic traits in livestock
- Ovarian function and disorders
- Advancements in Transdermal Drug Delivery
- Pluripotent Stem Cells Research
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- CRISPR and Genetic Engineering
- Lipid Membrane Structure and Behavior
- Essential Oils and Antimicrobial Activity
- Reproductive Physiology in Livestock
- Microbial infections and disease research
- Reproductive Health and Technologies
- Insect and Pesticide Research
- Inflammasome and immune disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- Selenium in Biological Systems
- Viral Infectious Diseases and Gene Expression in Insects
- Hair Growth and Disorders
- Cell death mechanisms and regulation
- Tryptophan and brain disorders
- Bee Products Chemical Analysis
- Genetic Syndromes and Imprinting
Inner Mongolia University
2012-2025
Inner Mongolia Maternal and Child Health
2010-2014
Inner Mongolia Electric Power (China)
2012
Mongolian University of Life Sciences
2010
China Agricultural University
2007
Newmarket Equine Hospital
1999-2006
University of Cambridge
1999-2006
Institute of Livestock and Grassland Science
1999
Tokyo University of Agriculture
1993-1994
We investigated the development to blastocyst and subsequent live-offspring stages of in vitro-matured bovine oocytes intracytoplasmically injected with flow cytometrically sorted bull sperm heads. Bull heads, prepared by ultrasound sonication, were distinguished on basis their relative DNA contents using a cytometer/cell sorter modified for sorting sperm. By fluorescence situ hybridization, proportion confirmed as having Y specific fraction was 82%. Injection single heads (cultured 24 h)...
Abstract Background Inflammation and oxidative stress are considered crucial to the pathogenesis of depression. Rat models depression can be created by combined treatments chronic unpredictable mild (CUMS) lipopolysaccharide (LPS). Behaviors associated with could improved treatment mesenchymal stem cells (MSCs) owing immunomodulatory functions cells. Therapeutic potentials MSCs reverse pro‐inflammatory cytokines, proteins, metabolites were identified transcriptomic, proteomic, metabolomic...
Abstract Background Our previous studies demonstrated that tilmicosin-loaded hydrogenated castor oil solid lipid nanoparticles (Til-HCO-SLN) are a promising formulation for enhanced pharmacological activity and therapeutic efficacy in veterinary use. The purpose of this work was to evaluate the acute toxicity Til-HCO-SLN. Methods Two nanoparticle doses were used study ICR mice. low dose (766 mg/kg.bw) with tilmicosin 7.5 times clinic dosage below median lethal (LD 50 ) subcutaneously...
Abstract Background Pyroptosis is a recently identified pathway of caspase-mediated cell death in response to microbes, lipopolysaccharide, or chemotherapy certain types cells. However, the mechanism how pyroptosis regulated not well-established. Methods Herein, intracellular bacteria were detected by staining and laser confocal microscopy TEM. Live/dead imaging assay was used examine macrophage death. Western blot immunohistochemical protein changes. IFA identify typical budding vesicles...
Polycystic ovary syndrome (PCOS) is a heterogeneous endocrine and metabolic disorder in women, which characterized by androgen excess, ovulation dysfunction, polycystic ovary. Although the etiology of PCOS largely unknown, many studies suggest that aberrant DNA methylation an important contributing factor for its pathological changes. In this study, we investigated characteristics their impact on gene expression granulosa cells obtained from patients. Transcriptome analysis found...
Development of bovine oocytes after intracytoplasmic sperm injection (ICSI) was investigated. Oocytes were matured for 24-26 h in vitro and injected with isolated heads. When treated 7% ethanol (v/v) 5 min, 71.7% ICSI activated as shown by the resumption meiosis formation female pronuclei. However, 41.5% heads remained condensed at 18-20 into ooplasm. The incidence decondensing that male pronuclei this stage 15.1% 26.4%, respectively. A total 55.5% reached 2-cell following head 54.7%...
A florfenicol-loaded solid lipid nanoparticle (FFC-SLN) suspension was prepared by hot homogenisation and ultrasonic technique. The characterised for its release profile, stability, toxicity, the physicochemical properties of nanoparticles. Antibacterial activity evaluated in vitro vivo. results showed that mean diameter, polydispersity index zeta potential nanoparticles were 253 ± 3 nm, 0.409 0.022 47.5 0.21 mV, respectively. In profile FFC-SLN had sustained effect. minimum inhibition...
The study was designed to investigate the effects of cryopreservation on bovine, ovine, and goat sperm motility, acrosome structure, enzyme activity, fertilization ability. Percentage with hyaluronidase (HYD) activity detected by a modified sodium hyaluronate-gelatin membrane. N-α-benzoyl-DL-arginine-p-nitroanilide (BNPNA) method used assess (ACE). mean percentage integrity dropped significantly (P < 0.01) after cryopreservation. ACE bovine (100.48) higher than that ovine (57.88) or (50.30),...
The aim of this study was to investigate the effect electrical pulse, ethanol, and ionomycin combined with cycloheximide (CHX), cytochalasin B (CB), 6-dimethylaminopurine (6-DMAP) on parthenogenetic developmental competence in vitro matured porcine oocytes. In experiment 1, oocytes were treated direct current pulse (DC pulse) then incubated NCSU-23 medium supplemented CHX, 6-DMAP, CB + 6-DMAP for 6 h, respectively. rate blastocyst development DC group significantly higher than those other...
We recently reported that electrical activation followed by secondary chemical greatly enhanced the developmental competence of in vitro matured porcine oocytes fertilized intracytoplasmic sperm injection (ICSI). hypothesized treatment with disulfide bond reducing agents will enhance development embryos produced this ICSI procedure. examined effects glutathione (GSH), dithiothreitol (DTT), GSH or DTT combination heparin on DNA structure, paternal chromosomal integrity, pronuclear formation,...
Kunming mouse strain is widely used in China, and the superovulation was administrated with 10 IU PMSG combined hCG. In this study, effects of exogenous gonadotropins on mice embryo quality derived from superovulated were assessed. Female at 6-8-wk old 0, 5, 7.5 PMSG/hCG mated male mice. The embryos retrieved 2.5 d post coitum. No statistic difference observed for number 2-cell collected per between control 5 treatment group, but significantly increased group (P<0.05). average 4-cell 8-cell...
The effects of culture time for oocyte maturation and the injection conditions capacitated sperm (with/without manipulation medium) on subsequent morphological changes in male female nuclei development embryos derived from microfertilization bovine oocytes were investigated. rates cleavage to blastocyst stage increased with extension 18h 25h.The best results (2-cell: 71.4%, blastocysts: 11.4%) obtained cultured 25 h. Microinjection into without medium, resulted higher (P<0.05) developmental...
To clarify the effect of triploidy on development bovine embryos, one or 2 sperm were injected into mature oocytes and subsequent chromosome composi-tion investigated. The cleavage rates with using matured in vitro for 24 h 36.3% (69/190) 39.0% (53/136), respectively. Most embryos had arrested before 8-cell stage none devel-oped to morula stage, although three (1.6%) developed blastocyst stage. incidence triploid derived from 2-sperm injection was 12.9%, incidences diploid haploid 48.4%...
Various types of cell cycle organization occur in mammals. In this study, centrosome changes during meiosis horse oocytes, and first following fertilization, parthenogenesis nuclear transfer, were monitored. Cumulus oocyte complexes harvested from ovaries obtained slaughtered mares cultured vitro . Meiotic oocytes germinal vesicle (GV), breakdown (GVBD), metaphase I II (MI MII) stages selected at various set times maturation. Embryos the stage generated by subjecting MII to fertilization...
Owing to the lack of a breeding index for efficient and quick fertility evaluations Holstein bulls when using traditional or genome-wide detection methods, this study aimed determine whether in vitro fertilization (IVF) could be used as an indicator conception rate artificial insemination (AI).Conventional sexed frozen semen from nine were IVF AI.The AI rates each bull confirmed positively correlated between conventional semen. The correlation coefficient R values methods 0.73 0.97 semen,...
Most hinnies (female donkey×male horse) and mules horse×male donkey) are sterile with few reports of equine fertile hybrids. The main cause this sterility is thought to be a meiotic block spermatogenesis oogenesis. This study compared the developmental features testes histological analyses in male hinny those normal, stallion Jack donkey. Hinny showed thicker tunica albuginea, fewer blood vessels more connective tissue testis parenchyma than Although mean number seminiferous tubules was...
The CRISPR system enables precise editing in genomic DNA but relies on intracellular homology-directed recombination (HDR) repair pathways and is extremely inefficient. Base technology developed based the CRISPR/Cas9 builds three editors (BE) by fusing nucleases that have lost cutting activity with different base deaminases: Cytosine editor (CBE) Adenine (ABE) Glycosylase (GBE). These two types of can complete substitution C > T (G A) or A G (T C) at gene target sites without producing...