Mitochondria are involved in many cellular processes; mitochondrial dysfunctions have been associated with apoptosis, aging, and a number of pathological conditions, including osteoarthritis (OA). Mitochondrial proteins attractive targets for the study metabolism chondrocyte, unique cell type present mature cartilage, its role tissue degradation. Using proteomics approach based on two-dimensional DIGE MALDI-TOF/TOF mass spectrometric identification mitochondria- enriched protein fractions...

Biofilm formation is one of the main causes for persistence Acinetobacter baumannii, a pathogen associated with severe infections and outbreaks in hospitals. Here, we performed comparative proteomic analyses (2D-DIGE MALDI-TOF/TOF iTRAQ/SCX-LC–MS/MS) cells at three different conditions: exponential, late stationary phase, biofilms. These results were compared alterations proteome resulting from exposure to biofilm inhibitory compound (salicylate). Using this multiple-approach strategy,...

Osteoarthritis (OA) is the most common rheumatic pathology and characterized primarily by articular cartilage degradation. Despite its high prevalence, there no effective therapy to slow disease progression or regenerate damaged tissue. Therefore, new diagnostic monitoring tests for OA are urgently needed, which would also promote development of alternative therapeutic strategies. In present study, we have performed an iTRAQ-based quantitative proteomic analysis secretomes from healthy human...

Objective Early diagnosis of knee osteoarthritis (KOA) in asymptomatic stages is essential for the timely management patients using preventative strategies. We develop and validate a prognostic model useful predicting incidence radiographic KOA (rKOA) non-radiographic osteoarthritic subjects stratify individuals at high risk developing disease. Methods Subjects without signs according to Kellgren Lawrence (KL) classification scale (KL=0 both knees) were enrolled OA initiative (OAI) cohort...

Abstract Introduction Chondroitin sulfate (CS) and glucosamine (GS) are symptomatic slow-acting drugs for osteoarthritis (OA) widely used in clinic. Despite their widespread use, knowledge of the specific molecular mechanisms action is limited. The aim this work to explore utility a pharmacoproteomic approach identification molecules involved pharmacological effect GS CS. Methods Chondrocytes obtained from three healthy donors were treated with 10 mM and/or CS 200 μg/mL, then stimulated...

Abstract Introduction Chondroitin sulfate (CS) is a symptomatic slow-acting drug for osteoarthritis (OA) widely used in the clinic. The aim of this work to find proteins whose secretion from cartilage cells under proinflammatory stimuli (IL-1β) regulated by CS, employing novel quantitative proteomic approach. Methods Human articular chondrocytes released three normal cartilages were grown SILAC medium. When complete incorporation heavy isotope was achieved, stimulated with IL-1β 5 ng/ml or...

Chondroitin sulfate (CS) is a symptomatic slow acting drug for osteoarthritis (OA) widely used the treatment of this highly prevalent disease, characterized by articular cartilage degradation. However, little known about its mechanism action, and recent large scale clinical trials have reported variable results on OA symptoms. Herein, we aimed to study modulations in intracellular proteome secretome human cells (chondrocytes) treated with three different CS compounds, origin or purity, two...

Abstract Background The field of biomarker discovery, development and application has been the subject intense interest activity, especially with recent emergence new technologies, such as proteomics-based approaches. In proteomics, search for biomarkers in biological fluids human serum is a challenging issue, mainly due to high dynamic range proteins present these types samples. Methods reducing content most highly abundant have developed, including immunodepletion or protein equalization....

Osteoarthritis (OA) is the most common age-related rheumatic disease. Chondrocytes play a primary role in mediating cartilage destruction and extracellular matrix (ECM) breakdown, which are main features of OA joint. Quantitative proteomics technologies demonstrating very interesting power for studying molecular effects some drugs currently used to treat patients, such as chondroitin sulfate (CS) glucosamine (GlcN). In this work, we employed iTRAQ (isobaric tags relative absolute...

Human mesenchymal stem cells (hMSCs) can be triggered to differentiate toward chondrocytes and thus harbor great therapeutic potential for the repair of cartilage defects in osteoarthritis (OA) other articular diseases. However, molecular mechanisms underlying chondrogenesis process are still part unknown. In this work, we followed a double-stable isotope labeling by amino acids cell culture (SILAC) strategy evaluate quantitative modulation secretome isolated from bone marrow (hBMSCs) during...

The aim of this study was to determine the effects glucosamine on matrix metalloprotease (MMP) production, mitogen-activated protein kinase (MAPK) phosphorylation, and activator (AP)-1 transcription factor activation in human chondrocytes. immortalized cell line lbpva55 healthy chondrocytes (obtained from donors) were subjected challenge with 10 ng/ml IL-1beta after pretreatment 2.5 or mmol/l glucosamine. MMP mRNA expression levels evaluated using quantitative real-time PCR, production...

Chondrocytes are widely used as an in vitro model of cartilage diseases such osteoarthritis (OA). As the unique residents mature cartilage, they responsible synthesis and release proteins essential for a proper tissue turnover. In this work, stable isotope labeling with amino acids cell culture (SILAC) technique has been standardized primary human articular chondrocytes (HACs) quantitative proteomic analyses. Then, it employed to study those protein modifications caused by proinflammatory...

Osteoarthritis (OA) is a degenerative joint pathology characterized by articular cartilage degradation that lacks from efficient therapy. Since previous epidemiological data show high controversy regarding the role of smoking in OA, we aimed to evaluate effects nicotine (the most physiologically active compound tobacco) on joint.Secretome analyses, based metabolic labeling followed LC-MALDI-TOF/TOF analysis, were carried out using an vitro model inflammation (primary human chondrocytes...

Human mesenchymal stem cells (hMSCs), residing in bone marrow as well the synovial lining of joints, can be triggered to differentiate toward chondrocytes. Thus, hMSCs harbor great therapeutic potential for repair cartilage defects osteoarthritis (OA) and other articular diseases. However, molecular mechanisms underlying chondrogenesis process are still part unknown. In this work, we applied first time stable isotope labeling by amino acids cell culture (SILAC) technique quantitative...

Umbilical cord stroma mesenchymal stem cells were differentiated toward chondrocyte-like using a new in vitro model that consists of the random formation spheroids medium supplemented with fetal bovine serum on nonadherent surface. The was changed after 2 days to one specific for induction chondrocyte differentiation. We assessed this reverse transcriptase-polymerase chain reaction, flow cytometry, immunohistochemistry, and secretome analyses. purpose study determine which proteins...

Endometrial cancer (EC) is the most frequent gynecological cancer. Tumor dissemination affecting ∼20% of EC patients characterized at primary carcinoma by epithelial-to-mesenchymal transition (EMT) associated with myometrial infiltration. At distant sites, interaction circulating tumor cells (CTCs) microenvironment crucial for metastatic colonization, a participation extracellular vesicles (EVs). We comprehensively approached these and secondary sites to study impact EVs on efficiency CTCs...

Osteoarthritis (OA) is a pathology characterized by the loss of articular cartilage. In this study, we performed peptidomic strategy to identify endogenous peptides (neopeptides) that are released from human osteoarthritic tissue, which may serve as disease markers. With aim, secretomes and healthy cartilages obtained knee hip were analyzed shotgun peptidomics. This discovery step led identification 1175 different peptides, corresponding 101 proteins, products physiological or pathological...

In osteoarthritis (OA), impairment of cartilage regeneration can be related to a defective chondrogenic differentiation mesenchymal stromal cells (MSCs). Therefore, understanding the proteomic- and metabolomic-associated molecular events during chondrogenesis MSCs could provide alternative targets for therapeutic intervention. Here, SILAC-based proteomic analysis identified 43 proteins with metabolic pathways whose abundance was significantly altered OA human bone marrow (hBMSCs). Then,...