The WRKY transcription factors (TFs) are one of the largest families TFs in plants and play multiple roles plant development stress response. In present study, GmWRKY16 encoding a factor soybean was functionally characterized Arabidopsis. is nuclear protein that contains highly conserved domain C2H2 zinc-finger structure, has characteristics transcriptional activation ability, presenting constitutive expression pattern with relative levels over fourfold old leaves, flowers, seeds roots...

Sugarcane-soybean intercropping has been widely used to control disease and improve nutrition in the field. However, response of soil microbial community diversity structure is not well understood. Since corresponds quality plant health, a pot experiment was conducted with sugarcane intercropped soybean. Rhizosphere collected 40 days after sowing, MiSeq sequencing utilized analyze composition. Soil columns were assess influence on activity (soil respiration carbon-use efficiency:...

Introduction Cadmium (Cd) stress is a significant threat to soybean production, and enhancing Cd tolerance in the focus of this study. The WRKY transcription factor family associated with abiotic response processes. In study, we aimed identify Cd-responsive GmWRKY172 from investigate its potential for soybean. Methods characterization involved analyzing expression pattern, subcellular localization, transcriptional activity. To assess impact , transgenic Arabidopsis plants were generated...

Soybean is a major crop that produces valuable seed oil and protein for global consumption. Seed are regulated by complex quantitative trait loci (QTLs) have undergone intensive selections during the domestication of soybean. It essential to identify genetic components understand their mechanism behind in We report MOTHER-OF-FT-AND-TFL1 (GmMFT) gene classical QTL has been reported regulate content many studies. Mutation MFT decreased seeds weight both Arabidopsis soybean, whereas increased...

Cadmium (Cd) is a widespread pollutant that toxic to living organisms. Previous studies have identified certain WRKY transcription factors, which confer Cd tolerance in different plant species. In the present study, we 29 Cd-responsive genes Soybean [Glycine max (L.) Merr.], and confirmed 26 of those GmWRKY were up-regulated, while 3 down-regulated. We also cloned novel, positively regulated GmWRKY142 gene from soybean investigated its regulatory mechanism tolerance. was highly expressed...

The seed weight of soybean [Glycine max (L.) Merr.] is one the major traits that determine yield and closely related to size. However, genetic basis synergistic regulation unclear.To understand molecular for formation traits, present study focused on QTLs mapping size in different environments target genes mining.A total 85 associated with were identified using a recombinant inbred line (RIL) population developed from Guizao1×B13 (GB13). We also detected 18 environmentally stable QTLs. Of...

Salt stress has a detrimental impact on crop yield, quality, and profitability. The tau-like glutathione transferases (GSTs) represent significant group of enzymes that play crucial role in plant responses, including salt stress. In this study, we identified transferase family gene from soybean named GmGSTU23. Expression pattern analysis revealed GmGSTU23 was predominantly expressed the roots flowers exhibited concentration-time-specific response to Transgenic lines were generated subjected...

Waterlogging in soil can limit the availability of nitrogen to plants by promoting denitrification and reducing fixation nitrification. The root-associated microorganisms that determine at root-soil interface be influenced plant genotype type, which potentially alters uptake capacity waterlogged soils. In a greenhouse experiment, two soybean genotypes with contrasting capacities resist waterlogging stress were grown Udic Argosol Haplic Alisol soils without waterlogging, respectively. Using...

The number of seeds per pod (NSPP) is one the critical components that affect soybean yield (Liu et al., 2020). Ln, which encodes GmJAGGED1 (GmJAG1), a major locus regulating NSPP and leaflet shape in (Fang 2013; Jeong 2012). A single base pair substitution Ln from guanine (G) to cytosine (C) led an amino acid change conserved EAR motif GmJAG1, resulting loss function GmJAG1 ln can significantly increase 2013) has been widely used breeding. By introducing into Kedou 1, was increased by...

The WRKY transcription factors (TFs) are one of the largest families TFs in plants and play multiple roles plant growth development stress response. In this study, GmWRKY21 encoding a factor was functionally characterized Arabidopsis soybean. protein containing highly conserved domain C2H2 zinc-finger structure is located nucleus has characteristics transcriptional activation ability. gene presented constitutive expression pattern rich roots, leaves, flowers soybean with over 6-fold relative...

Wangshuibai is a Chinese landrace wheat with high level of resistance to fusarium head blight (FHB) and deoxynivalenol (DON) accumulation. Using an F7 population recombinant inbred lines (RILs) derived from the cross between Annong 8455 for molecular mapping quantitative trait loci (QTL) FHB resistance, proportion scabbed spikelets (PSS) DON content were assessed under field conditions. Composite interval revealed that two three QTL significantly associated low PSS content, respectively,...

Deciphering the hereditary mechanism of seed iron (Fe) and zinc (Zn) content in soybean is important sustainable to address "hidden hunger" that presently affects approximately 2 billion people worldwide. Therefore, order detect genomic regions related Fe Zn content, a recombinant inbred line (RIL) population with 248 lines was assessed four environments Quantitative Trait Loci (QTLs) content.Wide variation found environments, genotype, environment, genotype × environment interactions had...

Aluminum (Al) toxicity is an essential factor that adversely limits soybean (Glycine max (L.) Merr.) growth in acid soils. WRKY transcription factors play important roles responses to abiotic stresses. Here, GmWRKY81 was screened from genes were differentially expressed under Al treatment Al-tolerant Baxi10 and Al-sensitive Bendi2. We found significantly induced by 20 μM AlCl3 upregulated for 2 h. In different tissues, the expression of induced. 0–1 cm root tips, highest level. The...

Although sugarcane-soybean intercropping has been widely used to control disease and improve productivity in the field, response of soil fungal communities not fully understood. In this study, rhizosphere sugarcane soybean under monoculture systems were investigated using Illumina MiSeq sequencing ITS gene. Intercropping decreased alpha-diversity changed community composition compared monocultures. Taxonomic analyses showed that dominant phyla Ascomycota, Zygomycota Basidiomycota. The...

Soybean is an important crop that grown worldwide. Flowering time a critical agricultural trait determining successful reproduction and yields. For plants, light temperature are environmental factors regulate flowering time. typical short-day (SD) plant, many studies have elucidated the fine-scale mechanisms of how soybean responds to photoperiod. Low can delay soybean, but little known about detailed mechanism affects flowering. In this study, we isolated GmFLC-like from which belongs...

Phytophthora root rot (PRR) caused by sojae (P. sojae) is one of the most serious limitations to soybean production worldwide. The identification resistance gene(s) and their incorporation into elite varieties an effective approach for breeding prevent from being harmed this disease. A valuable mapping population 228 F8:11 recombinant inbred lines (RILs) derived a cross resistant cultivar Guizao1 susceptible BRSMG68 high-density genetic linkage map with average distance 0.81 centimorgans...

This research aimed to identify stable quantitative trait loci (QTL) associated with oil and protein content in soybean. A population of 196 recombinant inbred lines (RILs) derived from Huachun 2 × Wayao was used evaluate these target traits. high-density genetic linkage map constructed by using high-throughput genome-wide sequencing technology, which contained 3413 recombination bin markers spanned 5400.4 cM an average distance 1.58 between markers. Eighteen QTLs controlling were detected....

Cadmium (Cd) is a widely distributed heavy metal that toxic to plants and humans. Although silicon (Si) has been reported reduce Cd accumulation toxicity in plants, evidence on the functions of Si its mechanisms possible alleviation soybean are limited. Therefore, controlled experiment was conducted investigate impacts retention soybean. Here, we determined growth index, distribution, antioxidant activity systems Si, as well expression levels differentially expressed genes (DEGs) under...

Ethylene response factor (ERF) transcription factors constitute a subfamily of the AP2/ERF superfamily in plants and play multiple roles plant growth development as well stress responses. In this study, GsERF1 gene from wild soybean BW69 line (an Al-resistant Glycine soja line) was rapidly induced to aluminum stress. Quantitative real-time PCR (qRT-PCR) analysis showed that maintained constitutive expression pattern stress, with increased amounts transcripts detected roots. The putative...

Pod shattering is an important domesticated trait which can cause great economic loss of crop yield in cultivated soybean. In this study, we utilized two recombinant inbred line populations (RILs, CY, Huachun 2×Wayao; GB, Guizao 1×B13) to identify quantitative loci (QTLs) associated with pod soybean across multiple environments. A total 14 QTLs for were identified the RIL populations, had LOD scores ranging from 2.64 44.33 phenotypic variance explanation (PVE) 1.33 50.85%. One QTL qPS16-1,...

Powdery mildew (PM), caused by the fungus Microsphaera diffusa, causes severe yield losses in soybean [Glycine max (L.) Merr.] under suitable environmental conditions. Identifying resistance genes and developing resistant cultivars may prevent PM damage. In this study, analysis of F1, F2, F8:11 recombinant inbred line (RIL) populations derived from cross between Zhonghuang 24 (ZH24) Huaxia 3 (HX3) indicated that adult-plant (APR) to powdery cultivar (cv.) ZH24 was controlled a single...