A5025773918- Virus-based gene therapy research
- Glaucoma and retinal disorders
- Viral gastroenteritis research and epidemiology
- Intraocular Surgery and Lenses
- Gastric Cancer Management and Outcomes
- Genomics and Chromatin Dynamics
- Viral Infectious Diseases and Gene Expression in Insects
- Herpesvirus Infections and Treatments
- Retinal and Macular Surgery
- Ocular Surface and Contact Lens
- Esophageal and GI Pathology
- RNA Interference and Gene Delivery
- CRISPR and Genetic Engineering
- Retinal and Optic Conditions
- Epigenetics and DNA Methylation
- Corneal Surgery and Treatments
- Pluripotent Stem Cells Research
- Ophthalmology and Eye Disorders
- Colorectal Cancer Screening and Detection
- Gastrointestinal disorders and treatments
- Ocular Infections and Treatments
- Retinal Diseases and Treatments
- Esophageal Cancer Research and Treatment
- Vascular Malformations Diagnosis and Treatment
- Single-cell and spatial transcriptomics
AdventHealth East Orlando
2024-2025
Chitkara University
2024
AdventHealth Orlando
2022-2024
BC Cancer Agency
2023
Massachusetts Eye and Ear Infirmary
2010-2022
University of Toronto
2015-2022
Harvard University
2010-2022
Allama Iqbal Medical College
2022
Kansas City University
2022
MedStar Washington Hospital Center
2022
The recent emergence of highly virulent human adenoviruses (HAdVs) with new tissue tropisms underscores the need to determine their ontogeny. Here we report complete high quality genome sequences and analyses for all previously unsequenced HAdV serotypes (n = 20) within species D. Analysis nucleotide sequence variability these in conjunction another 40 prototypes, comprising seven species, confirmed uniquely hypervariable regions species. mutation rate among HAdV-Ds was low when compared...
Transcriptional enhancers are critical for maintaining cell-type–specific gene expression and driving cell fate changes during development. Highly transcribed genes often associated with a cluster of individual such as those found in locus control regions. Recently, these have been termed stretch or super-enhancers, which predicted to regulate identity genes. We employed CRISPR/Cas9-mediated deletion approach study the function several enhancer clusters (ECs) isolated mouse embryonic stem...
Transcriptional enhancers are critical for development and phenotype evolution often mutated in disease contexts; however, even well-studied cell types, the sequence code conferring enhancer activity remains unknown. To examine regulatory pluripotent stem cells, we identified genomic regions with conserved binding of multiple transcription factors mouse human embryonic cells (ESCs). Examination these revealed that they contain on average 12.6 factor site (TFBS) sequences. Enriched TFBSs a...
Genes within the E3 transcription unit of human adenoviruses modulate host immune responses to infection. A comprehensive genomics and bioinformatics analysis for 38 viruses adenovirus species D (HAdV-D) revealed distinct surprising patterns homologous recombination. Homologous recombination was identified in open reading frames CR1α, CR1β, CR1γ, similar that previously observed with genes encoding three major structural capsid proteins, penton base, hexon, fiber.
Human adenoviruses (HAdVs) are uniquely important "model organisms" as they have been used to elucidate fundamental biological processes, recognized complex pathogens, and remedies for human health. As HAdVs may effect asymptomatic or mild severe symptomatic disease upon their infection of respiratory, ocular, gastrointestinal, genitourinary systems. High-resolution genomic data enhanced the understanding HAdV epidemiology, with recombination an major pathway in molecular evolution genesis...
Glucagon-like peptide receptor agonists (GLP-1 RAs) are associated with delayed gastric emptying and may increase the risk of aspiration due to retained contents. There no guidelines on peri-endoscopic use GLP-1 RAs, real-world outcomes in an ambulatory setting remain unknown. This study reports data from center a large tertiary hospital.
The genome of human adenovirus (HAdV) D30 was sequenced in depth. Sequence assembly and analysis revealed two distinct viral sequences with identical hexon genes, which were the same as one previously reported for HAdV-D30. However, viruses found to be a recombinant HAdV-D29. Exclusive reliance on serum neutralization can lead mischaracterization adenoviruses miss coinfections. Whole-genome sequencing remains gold standard proper classification HAdVs.
ABSTRACT For DNA viruses, genetic recombination, addition, and deletion represent important evolutionary mechanisms. Since these alterations can lead to new, possibly severe pathogens, we applied a systems biology approach study the pathogenicity of novel human adenovirus with naturally occurring canonical penton base Arg-Gly-Asp (RGD) loop, thought be critical cellular entry by adenoviruses. Bioinformatic analysis revealed new highly recombinant species D (HAdV-D60). A synthesis in silico...
Introduction: Corneal infection is the most common cause of profound ocular morbidity leading to blindness worldwide. in children difficult diagnose and treat, as they are unwilling sometimes unable cooperate during active management. This study analyses prevalence, microbiology, demography, therapeutic visual outcome infectious microbial keratitis paediatric age group seen at a tertiary eye care hospital south India. Materials Methods: A retrospective review all cases presenting with...
Heat shock proteins (HSPs) play a critical role in many intracellular processes, including apoptosis and delivery of other to compartments. Small HSPs have been shown previously participate cellular functions, IL-8 induction. Human adenovirus infection activates signaling, involving particularly the c-Src mitogen-activated protein kinases [Natarajan, K., et al. (2003) J. Immunol. 170, 6234-6243]. HSP27 MK2 are also phosphorylated, c-Src, its downstream targets, p38, ERK1/2, c-Jun-terminal...
Abstract Background and study aims Alterations to interstitial cells of Cajal (ICC) collagen fibrosis have been implicated in the pathogenesis gastroparesis. We aimed evaluate feasibility safety pyloric muscle sampling during gastric peroral endoscopic myotomy (G-POEM) association between ICC density degree with clinical outcomes. Patients methods This was a single-center prospective gastroparetic patients who underwent G-POEM intraprocedural biopsies January 2022 April 2023. count estimated...
Abstract Crop varieties or genotypes of a given species are pivotal for agricultural production and ownership, management improvement their germplasm is great challenge. Its morphological identification requires time, cost descriptors often compromised statistically due to phenotypic plasticity. Development DNA based signature can overcome these limitations. There global need implement world trade organization (WTO) intellectual property rights (IPR) guidelines Plant Breeders Rights (PBR)...
Abstract Histone variants H2A.Z and H3.3 are epigenetic regulators of memory, but roles other not well characterized. macroH2A (mH2A) is a structurally unique histone that contains globular macrodomain connected to the region by an unstructured linker. Here we assessed if mH2A regulates memory this role varies for two mH2A-encoding genes, H2afy (mH2A1) H2afy2 (mH2A2). We show fear impaired in mH2A1, mH2A2-deficient mice, whereas both groups were non-aversive spatial task. However, impairment...
Forensic odontology necessarily involves the application of dentistry along with various other branches sciences which deals proper handling, examination, evaluation, and presentation dental evidences, that aids to investigate a crime deliver justice. Sex determination is part forensic an essential priority when traditional identification deceased becomes impossible.To determine by analysis Amelogenin gene using Polymerase Chain Reaction (PCR) method on Deoxyribose nucleic acid (DNA)...
In this case report, we describe the rare occurrence of bilateral conjunctival retention cysts in a child with Stevens-Johnson syndrome. The was managed conservatively as there were no functional or cosmetic problems.
Abstract Background The encoding of cell intrinsic drug resistance states in breast cancer reflects the contributions genomic and non-genomic variations requires accurate estimation clonal fitness from co-measurement transcriptomic data. Somatic copy number (CN) variation is dominant mutational mechanism leading to transcriptional notably contributes platinum chemotherapy states. Here, we deploy time series measurements triple negative (TNBC) single-cell transcriptomes, along with...
Continuous epidural infusion of Bupivacaine and Ropivacaine with or without the addition Fentanyl has been evaluated by various researchers for effective postoperative pain relief. Studies however, depict significant variability in their results regard to analgesic efficacy adverse effects like hypotension, motor blockade etc.To comparatively evaluate efficacy, sparing effect, haemodynamic variations total consumption first 24 hours.A randomised double blind study was conducted on 100 adult,...