Cell sheets have immense potential for medical and pharmaceutical applications including tissue regeneration, drug testing, disease modelling. In this study, composite hydrogels were prepared from a mixture of phenolated hyaluronic acid (HA-Ph) gelatin (Gelatin-Ph), with controlled degree polymer crosslinking degradation, to fabricate muscle cell myoblasts. These obtained via hydrogen peroxide (H2O2)-mediated catalysed by horseradish peroxidase (HRP) peroxide-mediated cleavage the chains....

The cell cycle is known to be regulated by features such as the mechanical properties of surrounding environment and interaction cells with adhering substrates. Here, we investigated possibility regulating cell-cycle progression on gelatin/hyaluronic acid composite hydrogels obtained through hydrogen peroxide (H2O2)-mediated cross-linking degradation polymers varying exposure time H2O2 contained in air. stiffness hydrogel varied time. Human cervical cancer (HeLa) mouse mammary gland...

In vitro network formation by endothelial cells serves as a fundamental model for studies aimed at understanding angiogenesis. The morphogenesis of these to form is intricately regulated the mechanical and biochemical properties extracellular matrix. Here effects modulating in hydrogels derived from phenolated hyaluronic acid (HA-Ph) gelatin (Gelatin-Ph) are presented. Visible-light irradiation presence tris(2,2'-bipyridyl)ruthenium(II) chloride hexahydrate sodium persulfate induces...

Engineering skeletal muscle tissue in vitro is important to study the mechanism of myogenesis, which crucial for regenerating cells. The physicochemical properties cellular microenvironment are known govern various cell behaviours. Yet, most studies utilised synthetic materials model extracellular matrix that suffers from cytotoxicity We have previously reported property hydrogels obtained horseradish peroxidase (HRP)-catalysed cross-linking could be controlled by a simple adjustment...

Hydrogen peroxide (H2O2) is widely used for the gelation of aqueous solutions gelatin derivatives with phenolic hydroxyl groups (Gelatin-Ph) catalyzed by horseradish peroxidase (HRP). Apart from this, H2O2 known to cause degradation/depolymerization various polymers. Here, we prepared Gelatin-Ph hydrogels containing and HRP continuously supplying gas phase investigated mechanical properties resultant behaviors rat fibroblast human adipose-derived stem cells on them. Young's modulus hydrogel...

The development of hydrogels that allow vascular endothelial cells to form capillary-like networks is critical for advancing tissue engineering and drug discovery. In this study, we developed composed phenolated hyaluronic acid (HA-Ph) with an average molecular weight 490–159 kDa via sonication in aqueous solution. These were synthesized by the horseradish peroxidase-catalyzed crosslinking phenol moieties presence hydrogen peroxide gelatin. sonication-degraded HA-Ph (198 kDa) significantly...

Microencapsulation is a promising technology for stem cell therapy and tissue regeneration. Our previous work proposed the application of microencapsulation improving allogeneic hematopoietic transplantation. In this current work, we developed feasible method encapsulating human umbilical cord blood-derived cells. The cells were initially entrapped in ionic cross-linked alginate beads based on conventional method. It was noticed that easily dissolved phosphate-buffered saline with Ca2+/Mg2+,...

A liver organoid is an in vitro reconstruction of the that mimics vivo microstructure and performs functions. Liver organoids can be used for drug testing, as a model disease pathogenesis, bioartificial prototype material to develop promising alternative therapies failure. In this study, we reconstructed using primary rat hepatocytes, hepatic stellate cell line (LX2), human umbilical cord-mesenchymal stem cells (UC-MSCs), cord blood (UCB)-CD34+ hematopoietic stem/progenitor cells....

The study of the capillary-like network formation human umbilical vein endothelial cells (HUVECs) in vitro is important for understanding factors that promote or inhibit angiogenesis. Here, we report behavior HUVECs on composite hydrogels containing hyaluronic acid (HA) and gelatin with different degrees degradation, inducing physicochemical properties hydrogels. were obtained through horseradish peroxidase (HRP)-catalyzed hydrogelation consuming hydrogen peroxide (H2O2, 16 ppm) supplied...

As an agricultural-based village, wives in Mojorejo village located Ponorogo, East Jave have been supporting the family income from occassional production of bamboo handicraft, e.g. ‘besek’, ‘tumbu’, and ‘rinjing’ for generations. Despite its great potential, this handicraft is still produced individually with one only type product, resulting low economical impact village. This community service program aimed to establish a ‘bamboo center’ final goal increasing Village. uses participatory...

Horseradish peroxidase (HRP)-mediated extrusion bioprinting has a significant potential in tissue engineering and regenerative medicine. However, they often face challenges terms of printing fidelity structural integrity when using low-viscosity inks. To address this issue, method that alternately extrudes bioinks support material was developed study. The consisting cells, HRP, phenolated polymers, the contained hydrogen peroxide (H2O2). not only prevented collapse constructs but also...

Hematopoietic stem cells (HSCs) are the progenitor for all blood types including leukocytes, erythrocytes and platelets. Normally, human body is programmed to maintain sufficient level of hematopoietic or derivatives. However, in certain conditions, repeated chemotherapy, leukemic cancer, genetic mutation, bone marrow disorders; HSC transplantation becomes necessary. deals with two major obstacles: leukocyte antigen (HLA) matching high cell dose. Non-match activates immune reaction induces...

Suitable biomechanical properties with a degradation rate parallel to normal bone healing time are vital characteristics for biodegradable implant material in orthopaedics. Magnesium (Mg) is natural micronutrient as well metal close that of the human bone, while carbonate apatite (CO3Ap) biological good osteoconductivity which allows without forming fibrotic tissue. We fabricated Mg-CO3Ap composite various content ratios by powder metallurgy, milling times (3, 5, and 7 hours) at 200 RPM,...

Engineering the surfaces of biological organisms allows introduction novel functions and enhances their native functions. However, studies on surface engineering remained limited to unicellular organisms. Herein, nematode are engineered through in situ hydrogelation mediated by horseradish peroxidase (HRP) anchored cuticles. With this method, hydrogel sheaths approximately 10-μm thickness fabricated from a variety polysaccharides, proteins, synthetic polymers. Caenorhabditis elegans Anisakis...

Horseradish peroxidase (HRP)-mediated hydrogelation, caused by the cross-linking of phenolic groups in polymers presence hydrogen peroxide (H2O2), is an effective route for bioink solidification 3D bioprinting. Sugar beet pectin (SBP) naturally has cross-linkable phenols through enzymatic reaction. Therefore, chemical modifications are not required, unlike various that have been used system. In this study, we report application SBP extrusion-based bioprinting including HRP-mediated...

Recently, surface engineering of the cell membrane with biomaterials has attracted great attention for various biomedical applications. In this study, we investigated possibility modulating cycle progression using alginate and gelatin-based hydrogel sheaths a thickness ∼1 μm. The sheath was formed on surfaces through cross-linking catalyzed by horseradish peroxidase immobilized surface. did not decrease viability (>95% during 2 days culture) human cervical carcinoma line (HeLa) expressing...

Bone morphogenetic protein 2 (BMP-2) is a or growth factor that plays an important role in osteogenic differentiation. Recently, there increasing demand for BMP-2, as it the only osteoinductive approved by Food and Drug Administration (FDA) substitute bone grafts. However, recombinant BMP-2 production suffers from low yields changes biological function of due to efficiency translocation hosts. One key regulators influences signal peptide (SP). SP regulates process post-translational...

Protocols for isolation, characterization, and transplantation of hematopoietic stem cells (HSCs) have been well established. However, difficulty in finding human leucocyte antigens (HLA)-matched donors scarcity HSCs are still the major obstacles allogeneic transplantation. In this study, we developed a double-layered microcapsule to deliver paracrine factors from non-matched or low-matched other cells. The umbilical cord blood-derived progenitor cells, identified as CD34+ were entrapped...

Kelompok dan perilaku kelompok telah menjadi fokus utama dalam berbagai studi di bidang, termasuk psikologi, sosiologi, manajemen. Pembahasan tentang teori memiliki dampak yang signifikan memahami interaksi antar individu suatu kelompok. Studi ini bertujuan untuk mengkaji pembentukan kelompok, konteks serta faktor-faktor memengaruhi dinamika Selain itu, tujuan lainnya adalah menganalisis tahap perkembangan implikasinya situasi, baik organisasi maupun masyarakat. Metode penelitian digunakan...